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Search for "FUSARIUM|solani": 45 articles found in Index.

FRAGNER P., MIŘEJOVSKÝ P. (1990): Key to histological identification of causative agents in systemic mycoses II.  Česká Mykologie 44(2): 65-76   (published: 22nd June, 1990)
  abstract   Based on authors’ experience and data from literature the key suggests identification of microscopical fungi according to morphology of mycotic elements found in histological sections from human and animal tissues. Part II: Concise information about species and varieties comprising remarks on occurrence and character of diseases in humans and animals, morphology of fungi in tissues and histopathological pictures.

FRAGNER P., MIŘEJOVSKÝ P. (1989): Key to histological identification of causative agents in systemic mycoses I.  Česká Mykologie 44(1): 1-13   (published: 22nd February, 1990)
  abstract   Based on authors' experience and data from literature the key suggests identification of microscopical fungi according to morphology of mycotic elements found in histological sections from human and animal tissues. Part I: Introduction. Extent and contents of the key. Diagnostic features. Survey of taxa. Key.

OTČENÁŠEK M., DVOŘÁK J. (1985): Fungi infecting man. Taxonomy of agents of human mycoses in an alphabetical survey.  Česká Mykologie 39(3): 155-164   (published: 1985)
  abstract   An orientational survey of taxonomy of fungi which have been described as agents of human mycoses is given. An alphabetical list of 163 agents contains data on synonyms of the individual species and organs afflicted by them. The literature citation of the earliest paper calling attention to pathogenicity is provided. Current nomenclature of anamorph and teleomorph stages and their mutual relation is discussed.

KOCKOVÁ A., KUTKOVÁ M., PETROVÁ M. (1958): Die Arten der Gattung Fusarium, welche die Herzfäulnis der Zuckerrübe im Jahre 1956 in der Slowakei verursachten.  Česká Mykologie 12(2): 83-94   (published: 20th April, 1958)

MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt.   [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS]  Czech Mycology 70(1): 1-32   (published: 26th January, 2018)
  abstract   The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).

ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt.   [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris]  Czech Mycology 68(2): 183-214   (published: 20th December, 2016)
  abstract   An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.

PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review.   [biological control, postharvest diseases, microbial pesticides, rosaceous fruits]  Czech Mycology 68(1): 41-66   (published: 1st February, 2016)
  abstract   This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.

ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri.   [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism]  Czech Mycology 67(1): 1-22   (published: 10th April, 2015)
  abstract   Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.

ISMAIL M.A., ABDEL-HAFEZ S.I.I., HUSSEIN N.A., ABDEL-HAMEED N.A. (2013): Contribution to physiological and biochemical diagnostics of Fusarium taxa commonly isolated in Egypt.   [Fusarium, taxonomy, physiological features, acetylmethylcarbinol, diagnostic]  Czech Mycology 65(1): 133-150   (published: 10th June, 2013)
  abstract   Fusarium isolates belonging to 24 species and 8 sections were subjected to 11 growth, physiological and biochemical tests (166 isolates). All species were able to grow on 25% Glycerol nitrate agar (G25N), Mannitol medium, Czapek iprodione dichloran agar (CZID) and Dichloran chloramphenicol peptone agar (DCPA). Growth rate, colony colour and reverse on G25N and CZID were promising diagnostic criteria for separating species within sections. The growth rate and reverse on Mannitol agar was diagnostic not only for separation of F. oxysporum from F. solani, but also for separation of many other Fusarium species treated. The ability to grow on Tannin-sucrose agar could distinguish some species of sections Liseola and Sporotrichiella. Acid production on Creatine-sucrose agar distinguished some species of section Liseola and F. trichothecioides of section Discolor. Acetylmethylcarbinol production revealed for the first time that all isolates of F. subglutinans can produce this substance, while most remaining isolates lacked this ability. Tests for peroxidase, urease and pyrocatechol oxidase proved to be very useful. The phosphatase enzyme seems to be common in Fusarium species and therefore it is only diagnostic for F. camptoceras (negative results). Some physiological features could be used as criteria to distinguish some sections or species within the same section.

SAWMYA K., VASUDEVAN T.G., MURALI T.S. (2013): Fungal endophytes from two orchid species - pointer towards organ specificity.   [fungal endophytes, environment, orchids, Bulbophyllum neilgherrense, Pholidota pallida]  Czech Mycology 65(1): 89-101   (published: 10th June, 2013)
  abstract   Fungal endophytes may influence plant communities by altering the host’s fitness either positively or negatively. Little is known, however, about their host/organ specificity, life style and role in plantfungus symbiosis under varying environmental conditions. We compared the leaf and root endophyte assemblages of two orchids (Bulbophyllum neilgherrense and Pholidota pallida) from natural forests and greenhouse conditions. Xylariaceae species were consistently associated with leaf and root tissues, while Guignardia and Pestalotiopsis were found predominantly in the leaf tissues of both orchids. Correspondence analysis of the endophyte assemblages showed that the endophytes exhibited distinct organ but little host specificity. More endophytes were shared by the two different orchids growing in the same location when compared to endophyte assemblages of a single orchid from different locations. Considering the influence of endophytes in shaping the host’s community, diverse habitats must be screened vigorously to address questions regarding the role of endophytes in hostendophyte interactions.

TALIGOOLA H.K., ISMAIL M.A., CHEBON S.K. (2011): Mycobiota and aflatoxins associated with imported rice grains stored in Uganda.   [rice grain, xerophilic fungi, nephrotoxigenic penicillia, Fusarium, aflatoxins]  Czech Mycology 63(1): 93-107   (published: 10th August, 2011)
  abstract   Milled rice grains imported into Uganda from Pakistan were investigated for natural contamination by fungi and aflatoxins. The direct plating method using five isolation media was used to enumerate and isolate the fungi during a 270-day storage period. Fungi were isolated and identified to species level and the percentage contamination levels were calculated. A total of 35 species belonging to 16 genera were recorded. The broadest species spectrum were found in the genera Aspergillus, Penicillium, Eurotium and Fusarium, which were represented by 11, 7, 4, and 3 species, respectively. Throughout the storage period, xerophilic fungi including Aspergillus candidus, Eurotium amstelodami and E. chevalieri were predominantly isolated. Species of the genus Penicillium (particularly P. pinophilum) and its teleomorph Talaromyces ranked second in predominance, while Aspergillus flavus, Fusarium spp. and other field fungi occurred only sporadically. Aflatoxins were recorded in rice samples during most storage periods with one sample recording 20–50 ppb. The moisture content increased in rice grains attaining values of over 14 % from the 180th day of storage onwards. A positive correlation was observed between moisture content and incidence of xerophiles, including A. candidus and E. amstelodami.

ABDULLAH S.K., MONFORT E., ASENSIO L., SALINAS J., LOPEZ LLORCA L.V., JANSSON H.B. (2010): Soil mycobiota of date palm plantations in Elche, SE Spain.   [soil saprotrophic fungi, phytopathogenic fungi, Phoenix dactylifera, isolation methods]  Czech Mycology 61(2): 149-162   (published: 10th August, 2010)
  abstract   The mycobiota of soil from date palm (Phoenix dactylifera) plantations in Elche, SE Spain was examined using 23 soil samples and five isolation methods. One hundred and nineteen species assigned to 67 genera were isolated. The most frequent species were in decreasing order: Aspergillus fumigatus, A. niger, Neosartorya spinosa, Thielaviopsis punctulata, Chaetomium bostrychodes, Gilmaniella macrospora, Aspergillus candidus, Fusarium oxysporum, Rhizopus microsporus, Sordaria fimicola, Aspergillus terreus, Chaetomium murorum, Fusarium solani, Mucor racemosus, Penicillium citrinum and Thielaviopsis paradoxa. The thermotolerant and thermophilic species of Malbranchea cinnamomea, Myriococcum thermophilum, Rhizomucor miehei, Scytalidium thermophilum, Talaromyces emersonii, Thermoascus aurantiacus and Thermomyces lanuginosus were detected in various frequencies of occurrence. Our findings are compared with those from a similar survey of soil from date palm plantations in Iraq. Our study indicates that there is no characteristic mycobiota for soil in date palm plantations except for the more frequent isolation of some species well known as pathogens on date palm.

ISMAIL M.A., TALIGOOLA H.K., NAKAMYA R. (2008): Mycobiota associated with baby food products imported into Uganda with special reference to aflatoxigenic aspergilli and aflatoxins.   [mycobiota, aflatoxigenic aspergilli, aflatoxins, imported baby foods]  Czech Mycology 60(1): 75-89   (published: 4th July, 2008)
  abstract   Five baby food products imported into Uganda were collected from different shops and investigated for contamination by fungi and aflatoxins. Forty-two species belonging to 21 genera in addition to some unidentified fungi were recovered on dichloran rose bengal chloramphenicol agar. Cornflakes followed by Cerelac were the most heavily contaminated products while Heinz mixed cereal was the least. Cladosporium sphaerospermum, Fusarium tricinctum and Penicillium oxalicum were the most predominant fungi. On the other hand, using Aspergillus flavus/parasiticus agar, 34 % of the samples were positive for aflatoxigenic aspergilli. Aflatoxigenic aspergilli constituted 78.8 % of all aspergilli and 4.9 % of all contaminating fungi. Samples from all products were contaminated with aflatoxigenic aspergilli, but samples of Cerelac were the most, while those of Porridge oats were the least. Aflatoxin analysis of food samples revealed that 9 out of 13 analysed samples were contaminated with aflatoxins in the range of 1–10 ppb (6 samples) and 11–20 ppb (3 samples). Only samples of Cerelac were aflatoxin–free, although aflatoxigenic aspergilli were detected in some samples. It was noted that samples of Cerelac had the least moisture content compared to the other products. Contaminated foods constitute a health hazard to human consumption. These foods, especially those for babies, must therefore be examined at regular intervals in order to assess their hygienic quality.

LYSKOVÁ P. (2007): Saprotrophic microscopic fungi and dermatophytes accompanying infections of the skin and nails of patients in the Moravian-Silesian Region (Czech Republic).   [saprotrophic microscopic fungi, dermatophytes, superficial mycose, Scopulariopsis brevicaulis]  Czech Mycology 59(1): 125-137   (published: 28th June, 2007)
  abstract   Over a 19-month period, the spectrum of saprotrophic microscopic fungi isolated from 245 patients in the Moravian-Silesian Region (Czech Republic) was analysed. Saprotrophic microscopic fungi were isolated from nails (90 %) and skin (10 %). None was isolated from hair. The material was the most frequently positive for the presence of Scopulariopsis brevicaulis (32.6 %) followed by Cladosporium sphaerospermum (5.3 %), Aspergillus versicolor (4.0 %), Geomyces pannorum (4.0 %) and others.Dermatophytes and saprotrophic microscopic fungi were both studied within one year and represented 1110 isolates. Dermatophytes were isolated in most of the cases and represented 943 isolations (85 %). The saprotrophic microscopic fungus Scopulariopsis brevicaulis is a known causative agent of onychomycosis. In the evaluation including dermatophytes it ended in the 3rd position with 5.2 % of isolations behind Trichophyton rubrum (80 %) and T. mentagrophytes (8 %).

LABUDA R. (2005): Newly recorded Acremonium species from Slovakia: Acremonium atrogriseum, A. roseogriseum, A. spinosum, and Acremonium sp. (anamorph of Neocosmospora vasinfecta var. africana).   [fungi, soil, turkeylitter, Slovakia]  Czech Mycology 57(3-4): 239-248   (published: 10th February, 2006)
  abstract   Four species of the genusAcremonium (Ascomycota, Hypocreales), namely A. atrogriseum, A. roseogriseum, A. spinosum, and Acremonium sp. (teleomoiph Neocosmospora vasinfecta var. africana) hitherto not reported from Slovakia, are described and illustrated here.The former one was isolated from turkey litter, while the latter three were recovered from a soil sample. Representative strains of the fungi are deposited in the Microbiology Department Collection, SUA in Nitra.

HASHEM M. (2004): Biological control of two phytopathogenic fungal species isolated from the rhizoplane of soybean (Glycine max).   [biological control, soybean, Macrophominaphaseolina, Rhizoctonia solani]  Czech Mycology 56(3-4): 223-238   (published: 22nd December, 2004)
  abstract   Two hundred isolates representing 31 fungal species (20 genera) were recovered from soybean roots. Samples were collected from 12 localities at 3 different growth stages of the crop. The most dominant species were Aspergillus flavus, Fusarium oxysporum, Fusarium solani (Nectria haematococca), Macrophomina phaseolina and Rhizoctonia solani. Pathogenicity tests have proved the ability of Macrophomina phaseolina and Rhizoctonia solani to infect soybean roots and produce the symptoms of damping-off and root-rot diseases.The efficacy of three antagonists (Trichodermaharzianum, Epicoccum nigrum and Paecilomyceslilacinus) as well as two organic compounds (Strom and F-760) was evaluated as to their control of pathogenic fungi . Biocontrol fungi significantly suppressed Macrophominaphaseolina and Rhizoctonia solani in vitro and in vivo. Epicoccum nigrum and Paecilomyces lilacinus suppressed the growth of the pathogens by producing an inhibition zone while Trichoderma harzianum suppressed them by overgrowing. Strom and F-760 showed lower reduction effect of diseases in comparison with the antagonists.

NOVÁKOVÁ A., PIŽL V. (2003): Mycoflora in the intestine of Eisenia andrei (Oligochaeta, Lumbricidae) and in vermiculture substrates.   [cattle manure, saprotrophic and cellulolytic microfungi, earthworms]  Czech Mycology 55(1-2): 83-102   (published: 23rd July, 2003)
  abstract   Mycoflora of three commercial vermiculture systems based on cattle manure derived sub­ strates and Eisenia andrei earthworms was studied using several isolation methods. A total of 172 taxa of saprotrophic micromycetes were isolated (19 taxa of Zygomycetes, 9 taxa of Ascomycetes and 144 taxa of mitosporic fungi). Aspergillus fumigatus was the most frequent microfungal species in the intestine of Eisenia andrei. In vermicuture substrates, Aspergillus flavus and Aspergillus fumigatus were among species isolated very frequently by the soil dilution method, while Rhizopus stolonifer was estimated as frequent species using the soil washing isolation technique.

NOVOTNÝ D. (2003): A comparison of two methods for the study of microscopic fungi associated with oak roots.   [Quercus robur, oakdecline, Czech Republic, mycobiota, ophiostomatoid fungi]  Czech Mycology 55(1-2): 73-82   (published: 23rd July, 2003)
  abstract   Roots of four trees with symptoms of oak decline and roots of one healthy tree of Quercus robur were examined for the presence of fungi by using two methods (moist chambe rmethod and strong surface sterilisation method). Forty-five species were isolated in this project. Significant differences in composition of mycobiota based on the used method were detected. Fusarium solani, F. proliferatum, Sphaerostilbella aureonitens, Cylindrocarpon destructans, Pénicillium simplicissimum, P. purpurogenumvar. rubrisclerotium, Trichodermaviride, Ophiostomapiceae s.l. and Pénicillium glandicola were the most frequent fungi isolated by the moist chamber method. Cryptosporiopsis radicicola, dark sterile mycelium sp. 1, Cylindrocarpon destructans, ChaetomiuM globosum, Cylindrocarpon didymum, Pénicillium simplicissimum and Trichoderma koningii were dominant species observed by the method of strong surface sterilisation.

NOVOTNÝ D. (2002): Contribution to the knowledge of the mycoflora in roots of oaks with and without tracheomycotic symptoms.   [Quercus robur, Q. petraea, Q. rubra, oak decline, ophiostomatoid fungi, Penicillium, Czech Republic, Moravia]  Czech Mycology 53(3): 211-222   (published: 10th January, 2002)
  abstract   The mycoflora of roots of three species of oak (Quercus robur, Q. petraea, Q. rubra) from two localities near Moravské Budějovice (southwest Moravia, Czech Republic) with and without tracheomycotic symptoms was studied. Fifty-seven species of fungi were identified from the samples. The most frequently isolated were Fusarium solani, Penicillium gl and icola, P. glabrum, P. simplicissimum and Acremonium curvulum. In the roots of trees with tracheomycotic symptoms two species of ophiostomatoid fungi were recorded. Abiotic condiontions (insufieciency of precipitation and higher average temperature) are considered to be primary reasons of oak decline.

KUNERT J., NOVOTNÝ R. (2002): Degradation of human hair by three soil fungi. An electron microscopic study.   [keratinophilic fungi, keratinolysis, human hair, electron microscopy]  Czech Mycology 53(3): 189-201   (published: 10th January, 2002)
  abstract   Degradation of hair keratin has been studied in three soil fungi differing in keratinolytic ability, viz. Keratinophyton terreum, Dictyoarthrinopsis kelleyi and Fusarium moniliforme. All fungi attacked the hair cuticle forming specialised mycelial organs, fronds, under the scale-like cuticular cells. The cortex was attacked by very thin “boring hyphae”. Their growth was intracellular and perpendicular to the hair axis. In Keratinophyton terreum older boring hyphae branched into complex formations, displayingclearlytic action on keratin. In Dictyoarthrinopsis kelleyi branching was rare and lysis of keratin weaker. In Fusarium moniliforme, a fungus not regarded askeratinophilic, the growth of boring hyphaeceasedearly and the lyticaction remained minimal. All fungi digested the less keratinised parts of the hairs (endocuticle, intercellular substance, interfibrillar matrix) prior to the lysis of hard keratin fibrils.

MOSTAFA M.E., ZOHRI A.A., KOTBY R.S. (2001): Lipid, sterol and ergosterol accumulation in isolates of dematiaceous Hyphomycetes.   [Lipid, sterol, ergosterol, dematiaceous hyphomycetes]  Czech Mycology 53(2): 149-159   (published: 20th February, 2002)
  abstract   Mycelial dry weight, lipid and sterol contents of fungi tested varied with fungalgenus, species and even with isolate of one species. Their dry mass fluctuated between lll.6±l0.7-457.0±41.5 mg/50 ml medium. Lipids, sterols and ergosterol accumulated by the isolates tested ranged from 4.52±0.5-29.04±2.76%, 1.23±0.16-10.63±l.24% and 0.43±0.057-7.13±0.695% of their dry mass, respectively. Cochliobolus spicifer isolate No. 35 was the highest lipid-producer while Ulocladium atrum No. 90 proved to be superior in the production of sterols and ergosterol. TLC technique and chemical analysis of lipid classes produced by U. atrium No. 90 revealed that the lipid fractions are composed of free sterols, free fatty acids, sterol esters, glycolipids, phospholipids and squalene.

GHERBAWY Y.A.M.H., PRILLINGER H. (2000): Root mycoflora of pepper (Capsicum annuum) antagonistic to Verticillium dahliae.   [Frequency of root fungi, Verticillium dahliae, Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, Trichoderma harzianum, pepper seed germination]  Czech Mycology 52(3): 219-226   (published: 7th December, 2000)
  abstract   Thirty-two species belonging to 19 genera of fungi were collected from 30 soil samples from the rhizosphere of pepper plants. The fungal colonies were characterised using classical morphological methods following identification keys. The most frequently isolated fungi were Chaetomiumglobosum, Fusarium oxysporum, Gliocladium roseum, Mucor racemosus, Myrothecium verrucaria, Penicillium aurantiogriseum, P. expansum and Trichoderma harzianum. The crude culture filtrate of Verticillium dahliae at 100% concentration caused sharp decrease in pepper seed germination. Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, and Trichoderma harzianum produced a metabolite that retarded radial growth of Verticillium dahliae.

Colloquium "Fungi as Model Organisms in Research and Biotechnology", Olomouc, September 14th, 1999.  Czech Mycology 52(2): 139-178   (published: 20th April, 2000)
  abstract   The colloquium was organized by the joint Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology. The purpose of the colloquium was to provide a platform for a broad discussion on the use of fungi as model organisms in both basic and applied research. The programme of the colloquium was divided into four parts dealing with the following topics: phytopathology, biotechnology and ecology, physiology and biochemistry, and medical mycology. Each topic was opened with a plenary lecture (30 min.), followed by short communications (10 min.) and accompanied by poster presentations. Besides four plenary lectures, 15 short communications and 21 posters were presented. In total 38 researchers took part in the colloquium, at who discussed various questions of importance for experimental mycology until late in the evening. Abstracts of the contributions are given here.

GHERBAWY Y.A.M.H., ABDELZAHER H.M.A. (1999): Isolation of fungi from tomato rhizosphere and evaluation of the effect of some fungicides and biological agents on the production of cellulase enzymes by Nectria haematococca and Pythium ultimum var. ultimum.   [Biologicalcontrol, root-rot, fungicides Nectria haematococca, Pythium ultimum var. ultimum]  Czech Mycology 51(2-3): 157-170   (published: 25th May, 1999)
  abstract   Forty-five species and two species varieties belonging to twenty-six genera of fungi were isolated from 30 soil samples from the rhizosphere of tomato plants. The fungi most frequently isolated were Aspergillus flavus, A.fumigatus, A. niger, A. terreus, Gibberella fujikuroi, Nectria haematococca and Rhizopus stolonifer. Ridomil and Vitavax-captan (10, 50 and 100ppm) had no significant effects on the activity of Cl and Cₓ enzymes of Nectria haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but slightly decreased at 100ppm. Vitavax-captan (10, 50 and 100ppm) significantly decreased Cl enzyme activities of N. haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but at 100 ppm it showed a slightly inhibitory effect. Ridomil caused a slight increase in the activity of Cₓ and Cl enzymes by Pythium ultimum var. ultimum at low and moderate doses but the highest dose of Ridomil caused a slight reduction. Vitavax-captan slightly increased the activity of Cₓ and Cl enzymes in P. ultimum var. ultimum. Normal and sterilised filtrates of Myrothecium verrucaria, Penicillium oxalicum and Trichoderma harzianum induced a small decrease in Cl enzyme activity of Nectria haematococca. The sterilised filtrates of the three fungi tested caused greater inhibition compared to the normal filtrate. The production of Cₓ enzyme was slightly increased with normal and sterilised filtrates of Penicillium oxalicum and Trichoderma harzianum, but was significantly increased by both types of filtrates of Myrothecium verrucaria. The two types of filtrate of all fungi tested did not significantly affect the activity of Cl and Cₓ enzymes by lithium ultimum var. ultimum. Production of extracellular protein by Nectria haematococca was not significantly affected by any dose of the tested fungi cides. It was slightly increased by the two types of filtrate of the three tested fungi but significantly increased by the normal filtrate of Myrothecium verrucaria. The normal filtrate of all the fungi tested enhanced extracellular protein production to a greater extent than the sterilised filtrate. Extracellular proteins of Pythium ultimum var. ultimum were slightly increased by all doses of Vitavax-captan and low doses only of Ridomil, also two types of filtrate of all tested fungi caused a slightly increasing effect.

HUŇKOVÁ Z., KUBÁTOVÁ A., WEIGNEROVÁ L., KŘEN V. (1999): Induction of extracellular glycosidases in filamentous fungi and their potential use in chemotaxonomy.   [glycosidases, induction, Aspergillus, Penicillium, Fusarium]  Czech Mycology 51(1): 71-87   (published: 29th January, 1999)
  abstract   Data on the occurrence and inducibility of extracellular β-N-acetylhexosaminidase, α-galactosidase, α- and β-mannosidase and α-L-fucosidase, including inductors, are given for selected Aspergillus, Penicillium and Fusarium strains. These data represent additional information on the strains in the Culture Collection of Fungi, Department of Botany, Charles University, Prague, and in the Culture Collection of the Institute of Microbiology, Prague, Czech Republic, thus extending their usability in biochemistry and biotechnology. With respect to these biochemical data a taxonomic evaluation of the examined strains is presented. Several strains were re­ identified after biochemical and morphological comparisons with the type strains. The strains of A. niveus CCF 544, A. terreus CCF 76, CCF 869, and CCIM USA were re-identified as A. flavipes, the strain A. oryzae CCF 1301 as A. wentii.

OSTRÝ V., RUPRICH J., KOŽÍŠEK J. (1998): Determination of toxigenic Fusarium spp. in the domestic wheat - using the ICFM methodological recommendation.   [Fusarium spp., wheat, isolation, identification, mycotoxins, food mycology]  Czech Mycology 50(4): 313-323   (published: 12th July, 1998)
  abstract   Fifty one food wheat samples from three production regions in the Czech Republic have been mycologically examined in this study. Fusarium species were isolated by them ethod of grain rinse with sterile 0.1 % pepton in water and by the method of direct plating of grains after their surface sterilization. Czapek Dox Iprodione Dichlorane Agar (CZID) was used for cultivation. The methodological procedures used issued from there commendation of the International Commission of Food Mycology (ICFM). The identification of the isolated strains has been done accordingto Nelson et al. (1983) and by comparingwith collection strains of the genus Fusarium (Czech Collection of Microorganisms in Brno - CCM). Standardization of the above-mentioned mycological methods in food mycology is necessary for making collaborative studies and also for possibilities of comparison of results obtained in different time. Fusarium spp. isolated from food wheat samples of showed the greatest frequency in the following order: Fusarium graminearum, F. avenaceum, F. sporotrichioides, F. reticulatum and F. solani. The methodological procedure is recommended for determination of toxigenic Fusariumspp. in wheat and other cereals after a harvest and for comparison of results obtained both between individual production regions and in individual years.

ABDEL-MALLEK A.Y., MAZEN M.B., ALLAM A.D., HASHEM M. (1997): Specific responses of some phytopathogenic fungi to fungicides.   [fungicides, phytopathogenic species, Egypt]  Czech Mycology 50(1): 35-44   (published: 29th September, 1997)
  abstract   Laboratory experiments were carried out to examine the effect of four fungicides on spore germinating potentialities, radial growth and survival of viable propagules in soil of five phytopathogenic fungal species. The test organisms were achieved from infected roots of wheat plants cultivated in the Assiut area, Egypt. These were: Altemaria alternata, Cochliobolus sativus, Drechslera halodes, Fusarium moniliforme and F. oxysporum. The fungicides reduced germ tube production and radial growth of all fungi, and the reduction increased with increase in concentration. The maximal reduction was recorded at 50 μg/ml. At this concentration, Homai prevented spore germination of all test species. Neither F. moniliforme nor F. oxysporum can grow on agar medium supplemented with 50 μg/ml of either Benlate or Homai. The suppressive effect of fungicides on spore survival in soil cultures was also noticed but seemed to be lower than in agar application. In certain treatments, the numbers of viable propagules of tested fungi were not significantly affected in autoclaved nor non-autoclaved soil.

FRAGNER P. (1991): Identification of Aspergilli isolated from human and animal diseases I.  Česká Mykologie 45(3): 113-122   (published: 31st October, 1991)
  abstract   A suggestion of simple keys to determination of aspergilli found in human and animal diseases exploits the author’s experience and data from literature. Part I comprises: Introduction, Teleomorphs, Diagnostic possibilities of microscopical findings in clinical specimens, Cultivation, Remark on variability, List of species, Great key, Small key, and Description of species.

URBAN Z. (1991): Abstracts of papers delivered at the 8th conference of Czechoslovak mycologists held in Brno (August 28-September 1. 1989).  Česká Mykologie 45(1-2): 33-53   (published: 15th May, 1991)
  abstract   Abstracts of papers delivered at the 8th conference of Czechoslovak mycologists held in Brno August 28 — September 1,1989)

ŘEPOVÁ A. (1989): Soil micromycetes from Czechoslovakia - a list of isolated species with bibliography. II.  Česká Mykologie 43(4): 235-243   (published: 17th November, 1989)
  abstract   A list of micromycetes (saprophytic, keratinophilic, rhizosphere, nematophagous, ovicidal, dermatophytes, and cellular slime moulds) isolated from various Czechoslovak soils is presented. The paper includes distribution data and literature citations for each species. It is the second installment of the series and documents a wide variety of fungi, including Chaetomium species and other frequently encountered genera.

NOVOTNÁ J. (1989): Mikroskopische pilze auf Zysten Globodera rostochiensis Wollenw.  Česká Mykologie 43(2): 96-107   (published: 2nd May, 1989)
  abstract   The mycoflora of the potato cyst nematode Globodera rostochiensis Wollenweber 1925 was studied from four selected sites in Bohemia. A total of 36 fungal species were isolated. Opportunistic parasites included: Rhinocladiella anceps, Fusarium solani, Fusarium oxysporum, Cylindrocarpon sp., Humicola grisea, Gliocladium roseum and Phialophora fastigiata. Some fungi occurred widely across all sites, others were site-specific.

KOVÁČIKOVÁ E. (1988): Naučno-koordinacionálnaja konferencija SEV i simpozium „Issledovanije principov ustojčivosti rastěnij k bolezňjam i vreditěljam“.  Česká Mykologie 42(4): 227-232   (published: 16th December, 1988)

BRASLAVSKÁ O. (1984): Species of the genus Fusarium Link ex Fr. on potatoes in Slovakia.  Česká Mykologie 38(4): 243-245   (published: 1984)
  abstract   On infected potato tubers in Slovakia (1979–1981), seven Fusarium species were identified. F. sambucinum and F. solani were the most frequent.

STANĚK M. (1984): Microorganisms in the hyphosphere of fungi. I. Introduction.  Česká Mykologie 38(1): 1-10   (published: 10th February, 1984)
  abstract   The surface and immediate surroundings of fungal hyphae affected by their activity is termed the „hyphosphere“. Differences between microorganisms colonizing the hyphosphere and those colonizing a substrate is termed „hyphosphere effect“. The more extensive area of fungal effect on substrate, on the surface and surroundings of fruit-bodies, i.e. the environment affected by the activity of fungi is termed „mycosphere“. The nomenclature proposed is in agreement with the classification of the phytosphere of higher plants. The brief literary survey presents data on incidence of microorganisms in hyphosphere and on their importance, taking into account the results obtained in the Czechoslovak research institutions.

Abstracts of papers delivered at the 7th Conference of Czechoslovak mycologists held at České Budějovice, 13-18 September, 1982.  Česká Mykologie 37(2): 108-128   (published: 1983)
  abstract   Abstracts of papers delivered at the 7th Conference of Czechoslovak Mycologists, held at České Budějovice, 13–18 September 1982 - 1st Section: Taxonomy, Ecology and Mycofloristics

ČAČA Z. (1982): Present trends in the world phytopathological research.  Česká Mykologie 36(2): 65-71   (published: 15th May, 1982)
  abstract   Vararia granulosa (Fr.) Laurila is a name which cannot be applied to the fungus for which it is now commonly used. Fries (1838, 1874) considered the element representing our Vararia only a marginal part of his broad concept of Grandinia granulosa (Pers. ex Fr.) Fr. As there is no legitimate name for the fungus in question, Vararia borealis Pouz. spec. nov. is proposed for it here.

VINDUŠKA L. (1979): Fungi in Heterodera schachtii cysts.  Česká Mykologie 33(3): 170-175   (published: 1979)
  abstract   In 1976 and 1977 the occurrence of fungi in Heterodera schachtii Schmidt was determined. From 23.6% of cysts individual fungal species were isolated and determined. In 31.8% of cysts fungi did not occur, from 32.2% cysts bacterial colonies grew and in 12.4% of cysts mixtures of fungi and bacteria were found. From the parasitation point of view, the finding of Verticillium falcatum Petch, Cylindrocarpon destructans (Zinssmeister) Schölten, Humicola grisea Traaen, Fusarium oxysporum Schlecht, emend. Snyder et Hansen and Fusarium solani (Mart.) Appel et Wollenw. is important.

FASSATIOVÁ O. (1978): Soil micromycetes in abandoned fields in Bohemian Karst.  Česká Mykologie 32(4): 226-234   (published: 1978)
  abstract   An analysis of soil micromycetes in two abandoned fields in the Bohemian Karst during 1976–1977 revealed 64 species in the younger field (7 years) and 50 in the older (50 years). Dominant species in both were: Penicillium albidum, Fusarium solani, Humicola grisea, Absidia cylindrospora, and Mortierella sp. (section Polycephala). The best isolation method used nylon gauze buried in soil.

JANEČKOVÁ V., FASSATIOVÁ O., DANIEL M., KŘIVANEC K. (1977): Findings of soil microscopic fungi in the Himalaya Mountains (Nepal).  Česká Mykologie 31(4): 206-213   (published: 1977)
  abstract   Within the framework of the 1973 Czechoslovak expedition in the Makalu Mountain region in the Himalayas (East Nepal) a total of 43 soil samples were collected for mycologic examination. Fourteen genera with 37 species of soil fungi were isolated from the samples. Though the spectrum of the isolated microscopic fungi was not wide, it contained 2 genera (Acremonium and Tolypocladium) and 15 species described for the first time amongst the Himalayan mycoflora. Some of the species were collected in the area for the second time. Among the keratinophilic microscopic fungi, Chrysosporium lucknowense was isolated. The investigation and the collection of soil samples took place at the altitudes of 1000–4900 m in the sites where the presence of terrestrial mammals was established, or direct from lair openings. The aim was to detect potentially pathogenic or keratinophilic microscopic fungi. Various species of microscopic fungi were isolated for the first time from the extreme climatic conditions of the high mountains characterized by an increased occurrence of UV radiation, great temperature fluctuations, strong air flow, a decrease in the air pressure and oxygen content and extreme abiotic and biotic factors influencing the specific existence of the organisms.

NOVÁKOVÁ J. (1977): Wechselseitige Beziehungen zwischen den Virus- und Pilz- krankheiten bei Kulturpflanzen.  Česká Mykologie 31(2): 60-68   (published: 1977)
  abstract   The importance of mixed infections of cultivated plants by viruses and pathogenic fungi is analyzed both from theoretical and practical points of view. The course of root rots caused by pathogenic fungi in various virus-infected hosts is described as an example to demonstrate these relationships. The causes of the altered disease syndrome in the root system are discussed.

HOLUBOVÁ-JECHOVÁ V. (1970): The internal rots of Brazil nuts.  Česká Mykologie 24(4): 207-214   (published: 29th October, 1970)
  abstract   The author isolated and identified fungi causing the internal decay of Brazil nuts (Bertholletia excelsa). The fungi included Absidia blakesleeana, A. cylindrospora, Circinella muscae, Cunninghamella elegans, Rhizopus arrhizus, R. nigricans, R. oryzae, Aspergillus flavus, A. tamarii, A. wentii, Fusarium javanicum, Penicillium frequentans, P. cf. phialosporum, P. wortmanni, Trichoderma viride and Verticillium sp. These fungi were found in nuts with normal appearance but internal spoilage, and were responsible for rancidity and bitterness of the kernels.

FASSATIOVÁ O. (1970): Micromycetes unhabiting the mines of Příbram (Czechoslovakia).  Česká Mykologie 24(3): 162-165   (published: 20th July, 1970)
  abstract   Micromycetes isolated from uranium mines in Příbram include frequent pathogenic species like Aspergillus fumigatus and A. flavus and rare saprophytes such as Scopulariopsis acremonium, Geotrichum microsporum, Humicola brunnea var. africana and Tritirachium sp. nov. Isolations were made from various substrates and miners’ laryngeal swabs.

BENADA J. (1963): Einige Eigenschaften des orangeroten Pigmentes des Schneeschimmels - Fusarium nivale (Fr.) Ces. - und sein diagnostischer Wert.  Česká Mykologie 17(2): 98-101   (published: 9th April, 1963)

KOCKOVÁ-KRATOCHVÍLOVÁ A., VALOŠKOVÁ-KOTULOVÁ A. (1960): Das Vorkommen einer aktiven Giberella`s auf der Slowakei.  Česká Mykologie 14(3): 185-187   (published: 20th July, 1960)

FASSATIOVÁ O. (1958): Über die Variabilität der Gattungsmerkmale bei den Nebengattungen der imperfekten Pilze Fusarium Link und Cephalosporium Corda.  Česká Mykologie 12(1): 15-22   (published: 20th January, 1958)

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