To standardize conditions for the study of antibiotic activity in submerged culture of Melanconis flavovirens, the role of type, age, volume and storage period of inoculum was followed. Cultures derived from a submerged inoculum possessed both antifungal and antibacterial activity, while cultures originating from slants predominantly showed antibacterial activity. The age of the submerged inoculum appeared to be more important for potential antibiotic production than the period of storage at 4°C. The minimum volume of inoculum necessary to obtain antibiotically active cultures on submerged fermentation was estimated.
Kotilová-Kubičková L. (1982): Occurrence of amyloid substance in the plasma in hyphae of basidiocarps of some Amanita species (Agaricaceae). – Česká Mykologie 36(2): 114–117.
