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Search for "TRICHODERMA|atroviride": 8 articles found in Index.

AL-TOUBI A.S.S., AL-SADI A.M., AL-MAHMOOLI I.H., AL-HARRASI M.M.A., AL-SABAHI J.N., VELAZHAHAN R. (2022): Volatile organic compounds emitted by mycoparasitic fungi Hypomyces perniciosus and Cladobotryum mycophilum suppress the growth of Agaricus bisporus.   [antifungal, cobweb, ethanol, wet bubble, white button mushroom.]  Czech Mycology 74(2): 141-152   (published: 14th November, 2022)
  abstract   Hypomyces perniciosus and Cladobotryum mycophilum are mycoparasitic fungi infecting Agaricus bisporus and causing wet bubble and cobweb diseases, respectively. In this work, the role of volatile organic compounds (VOCs) emitted by these fungal mycoparasites in the suppression of A. bisporus was investigated. The VOCs of H. perniciosus and C. mycophilum effectively reduced the mycelial growth of A. bisporus by 60% and 73% after 5 days of incubation, respectively, compared to that of the control as assessed by the two-sealed-base-plates assay. Further, the VOCs of H. perniciosus and C. mycophilum were collected in a headspace solid-phase microextraction procedure, and their components analysed by means of gas chromatography-mass spectrometry. Ethanol was identified as the major volatile component in H. perniciosus and C. mycophilum. Ethanol vapour significantly retarded the growth of A. bisporus in an in vitro assay. The results of this study suggest that ethanol produced by H. perniciosus and C. mycophilum could be involved in the suppression of A. bisporus.

DEEPTHI A.S., RAY J.G. (2021): Ecological relevance of the endophytic fungal diversity in velamen roots of tropical epiphytic orchids.   [epiphytes, Orchidaceae, root-associated endophytes, Trichoderma, Endomelanconiopsis, Lasiodiplodia.]  Czech Mycology 73(1): 91-108   (published: 17th May, 2021)
  abstract   The velamen roots in epiphytic orchids are rather complex, and their environmental role remains poorly understood. Fungal associates are known in the velamen roots of tropical orchids, but the magnitude of their diversity in particular species remains unknown. The primary aim of this work was to explore endophytic fungal species associated with the velamen roots of three tropical epiphytic orchids. Velamen roots were collected from 30 plants of Rhynchostylis retusa and 25 plants each of Epidendrum radicans and Oncidium sphacelatum. Endophytic fungi were isolated from 2160 segments. Twenty species of velamen-root-associated fungal endophytes were separated with a culture technique. The isolated strains were grouped into morphotypes, subsequently identified morphologically and by means of ITS DNA barcoding. Ascomycota were the dominant group with 18 species. Trichoderma cf. asperellum, Endomelanconiopsis endophytica, Trichoderma cf. atroviride and Lasiodiplodia theobromae were the most frequent taxa isolated. A majority of the identified fungi were common to more than one orchid. Colonisation rate, isolation rate, Shannon-Wiener diversity index, species richness and species evenness of the endophytic fungi from different orchids were studied and were tested for significance with the Kruskal-Wallis H test. The colonisation rate and isolation rate of fungal associates in the velamen roots were found to be distinctly the lowest in Rhynchostylis retusa.

MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt.   [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS]  Czech Mycology 70(1): 1-32   (published: 26th January, 2018)
  abstract   The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).

ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt.   [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris]  Czech Mycology 68(2): 183-214   (published: 20th December, 2016)
  abstract   An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.

PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review.   [biological control, postharvest diseases, microbial pesticides, rosaceous fruits]  Czech Mycology 68(1): 41-66   (published: 1st February, 2016)
  abstract   This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.

NOVÁKOVÁ A., PIŽL V. (2003): Mycoflora in the intestine of Eisenia andrei (Oligochaeta, Lumbricidae) and in vermiculture substrates.   [cattle manure, saprotrophic and cellulolytic microfungi, earthworms]  Czech Mycology 55(1-2): 83-102   (published: 23rd July, 2003)
  abstract   Mycoflora of three commercial vermiculture systems based on cattle manure derived sub­ strates and Eisenia andrei earthworms was studied using several isolation methods. A total of 172 taxa of saprotrophic micromycetes were isolated (19 taxa of Zygomycetes, 9 taxa of Ascomycetes and 144 taxa of mitosporic fungi). Aspergillus fumigatus was the most frequent microfungal species in the intestine of Eisenia andrei. In vermicuture substrates, Aspergillus flavus and Aspergillus fumigatus were among species isolated very frequently by the soil dilution method, while Rhizopus stolonifer was estimated as frequent species using the soil washing isolation technique.

NOVOTNÝ D. (2003): A comparison of two methods for the study of microscopic fungi associated with oak roots.   [Quercus robur, oakdecline, Czech Republic, mycobiota, ophiostomatoid fungi]  Czech Mycology 55(1-2): 73-82   (published: 23rd July, 2003)
  abstract   Roots of four trees with symptoms of oak decline and roots of one healthy tree of Quercus robur were examined for the presence of fungi by using two methods (moist chambe rmethod and strong surface sterilisation method). Forty-five species were isolated in this project. Significant differences in composition of mycobiota based on the used method were detected. Fusarium solani, F. proliferatum, Sphaerostilbella aureonitens, Cylindrocarpon destructans, Pénicillium simplicissimum, P. purpurogenumvar. rubrisclerotium, Trichodermaviride, Ophiostomapiceae s.l. and Pénicillium glandicola were the most frequent fungi isolated by the moist chamber method. Cryptosporiopsis radicicola, dark sterile mycelium sp. 1, Cylindrocarpon destructans, ChaetomiuM globosum, Cylindrocarpon didymum, Pénicillium simplicissimum and Trichoderma koningii were dominant species observed by the method of strong surface sterilisation.

NOVOTNÝ D. (2002): Contribution to the knowledge of the mycoflora in roots of oaks with and without tracheomycotic symptoms.   [Quercus robur, Q. petraea, Q. rubra, oak decline, ophiostomatoid fungi, Penicillium, Czech Republic, Moravia]  Czech Mycology 53(3): 211-222   (published: 10th January, 2002)
  abstract   The mycoflora of roots of three species of oak (Quercus robur, Q. petraea, Q. rubra) from two localities near Moravské Budějovice (southwest Moravia, Czech Republic) with and without tracheomycotic symptoms was studied. Fifty-seven species of fungi were identified from the samples. The most frequently isolated were Fusarium solani, Penicillium gl and icola, P. glabrum, P. simplicissimum and Acremonium curvulum. In the roots of trees with tracheomycotic symptoms two species of ophiostomatoid fungi were recorded. Abiotic condiontions (insufieciency of precipitation and higher average temperature) are considered to be primary reasons of oak decline.

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