Microscopically (KOH with Parker ink) and semiquantitatively culturally ("ZOT" medium), 750 samples of skin scales from different areas of the body surface of 665 subjects (with or without dermatoses) were investigated for Malassezia furfur. This lipophilic yeast was isolated mostly from the capillitium, face and trunk (72–90% of samples). A total of 81.4% of samples were positive for M. furfur (65.6% microscopically and 73.5% culturally). In 57.7% of cases the yeast was observed by both methods. The average cultural detection was higher in samples with more microscopically visible blastoconidia. The results of M. furfur cultivation were influenced by several factors (e.g., subject preparation, timing of sample inoculation, cultivation conditions).
Volleková A. (1992): Microscopic and cultural proof of Malassezia furfur in skin scales. – Česká Mykologie 45(4): 164–171.
