A plate diffusion method was used to detect hydrolytic enzymes (lecithinaes, lipases, amylases, proteinases, milk clotting enzymes, urease) in mycelial cultures of saprotrophic macromycetes (Basidiomycotina). The results are demonstrated on 21 selected species. These tests can be used for screening high-yield strains and in chemotaxonomic studies.
Klán J., Baudišová D. (1990): Enzyme activity of mycelial cultures of saprotrophic macromycetes (Basidiomycotina). I. Methods of hydrolases estimation. – Česká Mykologie 44(4): 203–211.