Microscopic fungi survive in sterile, distilled water at laboratory temperature for various lengths of time, from less than 11 months (Kloeckera apiculata) to more than 11 years (Sporothrix schenckii). The survival times of some species as well as their spontaneous variability are given. Castellani’s “water cultures” are only an emergency solution for maintaining live collections. Some yeast species which are morphologically or biochemically similar have also similar survival times in water.
Fragner P. (1981): Further experience with Castellani’s „Water cultures“. – Česká Mykologie 35(3): 161–164.