A diagnostic system for the determination of yeasts occurring in human material on our territory is presented. It summarizes our experiences gained with more than 15,000 cultures during the last 20 years. According to our procedures it is possible to fairly exactly determine approximately 98% of yeasts within 48 hours or earlier, presuming that pure cultures are at hand. The key is based primarily on assimilation of glucose, galactose, sucrose, maltose and lactose, potassium nitrate assimilation, and macroscopic and microscopic appearance of the cultures. Some groups also use auxanograms of additional carbon sources and zymograms.
Fragner P. (1978): Yeasts in human material in our country and their differentiation. Part I. – Česká Mykologie 32(1): 32–42.