A method of fluorescence staining of hyphal nuclei in 32 species of fungi is described. It also permits observation of nuclei in conidia and septa. The latter can be safely distinguished by combining fluorescence under incident UV light with phase contrast under penetrating light. This method is applicable to quantitative evaluation of nuclear ratios in mycelium of Phycomycetes, Ascomycetes, Basidiomycetes, and Deuteromycetes.
Hejtmánek M., Hejtmánková N. (1976): Fluorescence microscopy of hyphal nuclei. – Česká Mykologie 30(1): 20–23.
