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TRICHODERMA":
97 articles found in Index.
AL-KHARUSI A.I., AL-SADI A.M., AL-SHUAIBI B., AL-MAHMOOLI I.H., AL-KINDI M.A., VELAZHAHAN R. (2025): Mass production of Trichoderma asperellum SQU7 using Phragmites australis as a substrate carrier for managing damping-off disease in cucumber. [biofungicide, biological control, common reed, Cucumis sativus, disease management, soilborne pathogen.] Czech Mycology 77(2): 109-120 (published: 25th July, 2025)
abstract
Trichoderma species are commonly used as biocontrol agents to manage various soilborne fungal and oomycete pathogens affecting crop plants. Various organic and inorganic carriers for mass production of Trichoderma intended for field application have been documented in the literature. In this study, Trichoderma asperellum strain SQU7, isolated from Omani soil, was screened for antagonistic activity against Pythium aphanidermatum, the causal agent of damping-off disease in cucumber. In addition, the possibility of using common reed (Phragmites australis) for mass production of T. asperellum was explored and its efficacy in controlling P. aphanidermatum-induced damping-off in cucumber was evaluated under controlled glasshouse conditions. The findings showed that T. asperellum provided a strong inhibitory effect on the growth of P. aphanidermatum in the confrontation assay and caused morphological abnormalities in the pathogen’s hyphae as determined by means of scanning electron microscopy analysis. Trichoderma asperellum proliferated well on common reed, and significantly suppressed P. aphanidermatum damping-off incidence in cucumber seedlings grown in pots. This study presents an efficient and affordable approach for the large scale production of T. asperellum, which can be utilised to effectively control damping-off of cucumber in an environmentally sustainable way.
WEI Y.-H., LIOU G.-Y., TZEAN S.-S., CHEN C.-C., HSIEH S.-Y. (2023): Trichoderma longicollum (Hypocreales), a new species from Taiwan. [coastal lagoons, mangrove soil, Trichoderma, phylogenetic analyses.] Czech Mycology 75(2): 153-158 (published: 21st November, 2023)
abstract
Coastal lagoons encompass various precious natural resources and are among the most productive ecosystems on Earth. A new Trichoderma species was identified during an investigation of fungi in mangrove soil in Qigu Lagoon, Taiwan. In this study, we provide the description, illustration, and phylogenetic analyses of this new species, Trichoderma longicollum.
NOVÁKOVÁ A., KUBÁTOVÁ A., VALINOVÁ Š., HUBKA V., KOLAŘÍK M. (2015): Trichoderma fassatiae, a new species from the section Pachybasium isolated from soil in the Czech Republic. [Ascomycota, Hypocreales, Trichoderma, morphology, DNA sequence data] Czech Mycology 67(2): 227-231 (published: 30th October, 2015)
abstract
Trichoderma fassatiae sp. nov. isolated from forest soil in the Czech Republic is described based on phenotype and DNA sequence data (ITS rDNA, RPB2, and TEF1α). It belongs to the Semiorbis clade in the section Pachybasium. Characters distinguishing it from similar species are provided.
KUBÁTOVÁ A., ČERNÝ M., NOVÁKOVÁ A. (2002): New records of micromycetes from the Czech Republic. IV. Acrodontium salmoneum, Chaunopycnis alba and Cylindrocarpostylus gregarius, and notes on Dactylaria lanosa and Trichoderma saturnisporum. [conidial fungi, saprotrophs, biologically active metabolites, Bohemia] Czech Mycology 53(3): 237-255 (published: 10th January, 2002)
abstract
Five saprotrophic mitotic microfungi, Acrodontium salmoneum, Chaunopycnis alba, Cylindrocarpostylus gregarius, Dactylaria lanosa, and Trichoderma saturnisporum, were found during studies of micromycete diversity in the Šumava Mts., Krkonoše Mts., Krušné hory Mts., Prague and Jevany near Prague in the Czech Republic. The microfungi were isolated from soil, except Cylindrocarpostylus gregarius, which was found on bark, and Acrodontium salmoneum isolated from air, seeds and mites. Three of them had not yet been reported from the Czech Republic and are considered to be the first records for this country. Descriptions and illustrations are given. All the fungi are maintained in the Culture Collection of Fungi (CCF), Department of Botany, Faculty of Science, Charles University, Prague.
MINTER D.W. (1981): Microfungi on needles, twigs and cones of pines in Czechoslovakia. Česká Mykologie 35(2): 90-101 (published: 1981)
abstract
One hundred and forty-seven species of microfungi are listed from rotting needles, twigs and cones of pines in Czechoslovakia (mostly from Bohemia). Thirty-seven of these are reported as new from this region. The Czechoslovak pine mycoflora is compared briefly with those of other countries and is found more varied than any previously recorded.
HOLUBOVÁ-JECHOVÁ V. (1970): The internal rots of Brazil nuts. Česká Mykologie 24(4): 207-214 (published: 29th October, 1970)
abstract
The author isolated and identified fungi causing the internal decay of Brazil nuts (Bertholletia excelsa). The fungi included Absidia blakesleeana, A. cylindrospora, Circinella muscae, Cunninghamella elegans, Rhizopus arrhizus, R. nigricans, R. oryzae, Aspergillus flavus, A. tamarii, A. wentii, Fusarium javanicum, Penicillium frequentans, P. cf. phialosporum, P. wortmanni, Trichoderma viride and Verticillium sp. These fungi were found in nuts with normal appearance but internal spoilage, and were responsible for rancidity and bitterness of the kernels.
KRUPODOROVA T., BARSHTEYN V., KIZITSKA T., RATUSHNYAK V., BLUME Y. (2023): Antagonistic activity of selected macromycetes against two harmful micromycetes. [ascomycetes, Aspergillus niger, basidiomycetes, dual-culture, Penicillium polonicum.] Czech Mycology 75(1): 85-100 (published: 23rd June, 2023)
abstract
Competition between 31 macromycete species and two harmful micromycetes Aspergillus niger and Penicillium polonicum was evaluated using dual-culture plate assay. All investigated fungi, except for Inonotus obliquus and Lepista luscina, possessed different levels of antagonistic activity against the tested micromycetes. Hypsizygus marmoreus and Lyophyllum shimeji were inactive against A. niger. Morchella esculenta and Oxyporus obducens were passive in the case of co-growth with P. polonicum. The study of fungal interactions showed variability in types of reactions and level of their visualisation. Co-cultivation of the studied species resulted in the following reactions: deadlock after mycelial contact and at a distance, partial or complete replacement after initial deadlock on contact. In general, the studied macromycetes showed moderate activity against the two micromycetes, as 11 species showed active and 7 species moderate antagonism. Results revealed higher antagonistic activity of macromycetes against P. polonicum than in A. niger co-cultures. Wood-decaying species Ganoderma lucidum and Trametes versicolor were the most active fungi against the two tested micromycetes with a maximal antagonism index. These findings provide valuable insights which can be further explored by means of in vivo assays to find a suitable agent for the biocontrol of diseases or spoilage caused by A. niger and P. polonicum.
GRISHKAN I., TEMINA M. (2023): Diversity of microfungal communities inside saxicolous lichens from Nahal Oren, Mount Carmel, Israel. [endolichenic fungi, lichen thallus, melanised fungi, microclimatic contrast, rock surface.] Czech Mycology 75(1): 15-34 (published: 28th March, 2023) Electronic supplement
abstract
In this study, we examined the endolichenic fungal communities of saxicolous lichens covering the rocks in the Nahal Oren valley, northern Israel. A total of 60 fungal species belonging to 35 genera were isolated from six lichen species collected in the summer and winter on the south-facing slope (SFS) and north-facing slope (NFS) of the valley. We verified that rocks serve as a possible source for the formation of endolichenic communities because communities colonising lichen thalli and the rock surface shared 39% of species and clustered together on the SFS. On the NFS, with a comparatively favourable microclimate, lichen thalli abundantly harboured typical soil fungi such as Clonostachys rosea and Fusarium spp. in winter and summer, respectively. At the same time, more severe environmental conditions on the SFS facilitated the prevalence of melanised fungi with thick-walled and multicellular spores irrespective of season. The lowest species richness and isolate densities of endolichenic communities were registered in the thalli of Collema cristatum. This decrease, especially expressed in the summer, was probably associated with the antifungal effect of substances produced by its cyanobiont, as well as with the heavy dehydration of thalli during the dry season.
KOUKOL O., MAGDALINOU E., PÁNKOVÁ H., BOROVIČKA J., MÜNZBERGOVÁ Z. (2022): Do microclimatic conditions in two forest types on serpentine bedrock affect culturable microfungi in pine litter needles? [fungal diversity, ITS rDNA, temperature and humidity, Pinus sylvestris.] Czech Mycology 74(2): 181-194 (published: 24th November, 2022)
abstract
Microfungi colonising coniferous needles in litter were intensively studied in previous decades, but forest stands on serpentine soils have been overlooked. Also, the effects of microclimatic conditions on fungal communities in coniferous litter are unknown. In our study, we aimed to characterise communities of culturable microfungi colonising pine litter needles collected from two types of Scots pine forest growing on serpentine bedrock, i.e. dense forest with relatively stable microclimatic conditions and open-canopy forest on exposed rock with highly variable conditions. The composition of their fungal communities was analysed in respect to microclimatic conditions at the collection sites. Using a combination of phenotypic and molecular data (sequences of ITS rDNA), 35 taxa were distinguished in 1078 fungal colonies recorded, out of which 25 were identified to the species level. Fungal communities were most affected by needle type (litter vs. fermentation layer) followed by maximum temperature during the previous five months. Interestingly, a higher number and abundance of species were recorded at the warmer site, in the open-canopy forest. Dominant fungi recorded in this study (Desmazierella acicola, Phacidium lacerum and Scleroconidioma sphagnicola) were mostly identical to those recorded in previous studies and the occurrence of less abundant taxa previously not recorded in pine litter suggests that the uppermost litter layer represents an important reservoir of fungal diversity.
AL-TOUBI A.S.S., AL-SADI A.M., AL-MAHMOOLI I.H., AL-HARRASI M.M.A., AL-SABAHI J.N., VELAZHAHAN R. (2022): Volatile organic compounds emitted by mycoparasitic fungi Hypomyces perniciosus and Cladobotryum mycophilum suppress the growth of Agaricus bisporus. [antifungal, cobweb, ethanol, wet bubble, white button mushroom.] Czech Mycology 74(2): 141-152 (published: 14th November, 2022)
abstract
Hypomyces perniciosus and Cladobotryum mycophilum are mycoparasitic fungi infecting Agaricus bisporus and causing wet bubble and cobweb diseases, respectively. In this work, the role of volatile organic compounds (VOCs) emitted by these fungal mycoparasites in the suppression of A. bisporus was investigated. The VOCs of H. perniciosus and C. mycophilum effectively reduced the mycelial growth of A. bisporus by 60% and 73% after 5 days of incubation, respectively, compared to that of the control as assessed by the two-sealed-base-plates assay. Further, the VOCs of H. perniciosus and C. mycophilum were collected in a headspace solid-phase microextraction procedure, and their components analysed by means of gas chromatography-mass spectrometry. Ethanol was identified as the major volatile component in H. perniciosus and C. mycophilum. Ethanol vapour significantly retarded the growth of A. bisporus in an in vitro assay. The results of this study suggest that ethanol produced by H. perniciosus and C. mycophilum could be involved in the suppression of A. bisporus.
NOVOTNÝ D. (2022): Contribution to the endophytic mycobiota of aerial parts of oaks. [endophytes, Czech Republic, Quercus robur, Quercus petraea, acorns, seeds, twigs.] Czech Mycology 74(2): 111-121 (published: 1st July, 2022)
abstract
The endophytic mycobiota inhabiting branches of Quercus petraea from two localities in the Křivoklát area, and branches, twigs and acorns of Quercus robur from one locality near the town of Semily were studied (all sites in the Czech Republic). Seventeen fungal taxa were isolated from branches of Q. petraea. Dominant fungi were found to be Colpoma quercinum and Phoma sp. as well as sterile dark mycelium and a black yeast-like fungus close to Sarcinomyces crustaceus. Eighteen fungal taxa were found in samples of aerial parts in Quercus robur. The most frequently isolated taxa were Pezicula cinnamomea, Colpoma quercinum, Alternaria alternata agg. and Acremonium sp.
DEEPTHI A.S., RAY J.G. (2021): Ecological relevance of the endophytic fungal diversity in velamen roots of tropical epiphytic orchids. [epiphytes, Orchidaceae, root-associated endophytes, Trichoderma, Endomelanconiopsis, Lasiodiplodia.] Czech Mycology 73(1): 91-108 (published: 17th May, 2021)
abstract
The velamen roots in epiphytic orchids are rather complex, and their environmental role remains poorly understood. Fungal associates are known in the velamen roots of tropical orchids, but the magnitude of their diversity in particular species remains unknown. The primary aim of this work was to explore endophytic fungal species associated with the velamen roots of three tropical epiphytic orchids. Velamen roots were collected from 30 plants of Rhynchostylis retusa and 25 plants each of Epidendrum radicans and Oncidium sphacelatum. Endophytic fungi were isolated from 2160 segments. Twenty species of velamen-root-associated fungal endophytes were separated with a culture technique. The isolated strains were grouped into morphotypes, subsequently identified morphologically and by means of ITS DNA barcoding. Ascomycota were the dominant group with 18 species. Trichoderma cf. asperellum, Endomelanconiopsis endophytica, Trichoderma cf. atroviride and Lasiodiplodia theobromae were the most frequent taxa isolated. A majority of the identified fungi were common to more than one orchid. Colonisation rate, isolation rate, Shannon-Wiener diversity index, species richness and species evenness of the endophytic fungi from different orchids were studied and were tested for significance with the Kruskal-Wallis H test. The colonisation rate and isolation rate of fungal associates in the velamen roots were found to be distinctly the lowest in Rhynchostylis retusa.
RAMSHAJ Q., RUSEVSKA K., TOFILOVSKA S., KARADELEV M. (2021): Checklist of macrofungi from oak forests in the Republic of Kosovo. [fungi, taxa, Balkan Peninsula, diversity, ecology, rare species.] Czech Mycology 73(1): 21-42 (published: 12th February, 2021) Electronic supplement
abstract
In the period from 2017 to 2019 a survey of the diversity of fungi in oak forests in the Republic of Kosovo was conducted. The survey included 31 localities, mainly in Quercetum frainetto-cerris and Querco-Carpinetum orientalis communities. As a result of the fieldwork performed in various seasons, a first checklist of fungi from oak forests in the country is provided. A total of 220 taxa (219 species and one forma) were identified. The majority of the identified taxa belong to Basidiomycota (206) and only 14 species to Ascomycota. The paper provides a list of all recorded species with data on locality, altitude, time of collection, forest association and type of substrate. Distribution and ecology of selected rare or threatened species are briefly discussed.
Book review - THOMAS LÆSSØE & JENS H. PETERSEN (2019): Fungi of Temperate Europe Czech Mycology 71(2): 230-232 (published: 20th December, 2019)
NOVOTNÝ D., LUKÁŠ J., BROŽOVÁ J., RŮŽIČKOVÁ P. (2019): Comparison of the occurrence of fungi causing postharvest diseases of apples grown in organic and integrated production systems in orchards in the Czech Republic. [Malus domestica, organic production, integrated production, Neofabraea, Penicillium.] Czech Mycology 71(1): 99-121 (published: 28th June, 2019)
abstract
During the years 2013–2015, the occurrence of fungi causing postharvest diseases of apples was evaluated in three apple orchards with integrated and organic production systems in the Czech Republic. The postharvest disease fungi were more frequently recorded in fruits from organically cultivated apple trees (average 29.97%) than those from integrated production (17.67%). This difference is statistically significant. Neofabraea, Penicillium, Alternaria and a sterile grey mycelium were the most frequently recorded taxa in apples grown in organic and integrated production systems. There were also significant differences in the frequency of occurrence of the most frequent fungal taxa between years. In 2013 and 2014, Neofabraea was the most frequently isolated genus in both cultivation systems, whereas in 2015, Penicillium was the most frequently recorded genus also in both cultivation systems, which was likely caused by the low precipitation in the 2015 growing season.
NOVÁKOVÁ A., KUBÁTOVÁ A., SKLENÁŘ F., HUBKA V. (2018): Microscopic fungi on cadavers and skeletons from cave and mine environments [European caves, abandoned mines, dead bodies, bones, mammals, frogs, spiders, isopods, micromycetes] Czech Mycology 70(2): 101-121 (published: 19th August, 2018)
abstract
During long-term studies of microscopic fungi in 80 European caves and mine environments many cadavers and skeletons of animals inhabiting these environments and various animal visitors were found, some of them with visible microfungal growth. Direct isolation, the dilution plate method and various types of isolation media were used. The resulting spectrum of isolated fungi is presented and compared with records about their previous isolation. Compared to former studies focused mainly on bat mycobiota, this paper contributes to a wider knowledge of fungal assemblages colonising various animal bodies in underground environments.The most interesting findings include ascocarps of Acaulium caviariforme found abundant on mammals cadavers, while Botryosporium longibrachiatum isolated from frogs, Chaetocladium jonesiae from bats and Penicillium vulpinum from spiders represent the first records of these species from cadavers or skeletons.
MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt. [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS] Czech Mycology 70(1): 1-32 (published: 26th January, 2018)
abstract
The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).
ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt. [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris] Czech Mycology 68(2): 183-214 (published: 20th December, 2016)
abstract
An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.
PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review. [biological control, postharvest diseases, microbial pesticides, rosaceous fruits] Czech Mycology 68(1): 41-66 (published: 1st February, 2016)
abstract
This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.
ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri. [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism] Czech Mycology 67(1): 1-22 (published: 10th April, 2015)
abstract
Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.
ONDRÁČKOVÁ E., ONDŘEJ M., PROKINOVÁ E., NESRSTA M. (2013): Mycoparasitic fungi reducing the incidence and virulence of Bipolaris sorokiniana. [mycoparasitic activity, mycoparasitic fungi, Clonostachys, Trichoderma, Bipolaris sorokiniana] Czech Mycology 65(1): 103-112 (published: 10th June, 2013)
abstract
The mycoparasitic efficiency of 28 strains/isolates of Clonostachys rosea f. rosea and Clonostachys rosea f. catenulata against the pathogenic fungus Bipolaris sorokiniana was determined in dual cultures on Czapek-Dox nutrient agar. Strains with low and medium efficiency were antagonistically inhibited by B. sorokiniana, and inhibitory zones were formed between the colonies of both fungi. The mycelium of Clonostachys strains with high efficiency overgrew and degraded B. sorokiniana colonies without formation of an inhibitory zone. In dual cultures, Trichoderma sp. and the most effective Clonostachys strains degraded B. sorokiniana colonies within 3 to 5 and 12 to 15 days, respectively.When rye seeds were treated with a mixture of C. rosea f. rosea, Trichoderma sp. and B. sorokiniana, development of B. sorokiniana on both seeds and seedlings was reduced when compared with a B. sorokiniana treatment. The treatment of rye seeds with Clonostachys and Trichoderma had a positive effect on seed germination and seedling length.
SAWMYA K., VASUDEVAN T.G., MURALI T.S. (2013): Fungal endophytes from two orchid species - pointer towards organ specificity. [fungal endophytes, environment, orchids, Bulbophyllum neilgherrense, Pholidota pallida] Czech Mycology 65(1): 89-101 (published: 10th June, 2013)
abstract
Fungal endophytes may influence plant communities by altering the host’s fitness either positively or negatively. Little is known, however, about their host/organ specificity, life style and role in plantfungus symbiosis under varying environmental conditions. We compared the leaf and root endophyte assemblages of two orchids (Bulbophyllum neilgherrense and Pholidota pallida) from natural forests and greenhouse conditions. Xylariaceae species were consistently associated with leaf and root tissues, while Guignardia and Pestalotiopsis were found predominantly in the leaf tissues of both orchids. Correspondence analysis of the endophyte assemblages showed that the endophytes exhibited distinct organ but little host specificity. More endophytes were shared by the two different orchids growing in the same location when compared to endophyte assemblages of a single orchid from different locations. Considering the influence of endophytes in shaping the host’s community, diverse habitats must be screened vigorously to address questions regarding the role of endophytes in hostendophyte interactions.
KOUKOL O. (2011): Early spring mycobiota of pine litter needles. [fungal diversity, fungal communities, microfungi, Pinus sylvestris] Czech Mycology 63(2): 153-161 (published: 10th August, 2011)
abstract
Rise in air temperature accompanied by snow-melting in early spring may affect fungi colonising litter needles. This study dealt with the diversity of communities of culturable microfungi colonising Scots pine litter at localities still covered by snow and after snowmelt. Surface-sterilised needles were cultivated on malt agar under two temperature regimes (8/9 and 15/16 °C) and outgrowing fungi were isolated into pure cultures and identified based on morphology and analysis of ITS and partial 28S rDNA regions. Thirty-one fungal species were isolated in total. Dominant colonisers with a colonisation frequency of over 10 % included Verticicladium trifidum, Ceuthospora pinastri, Sympodiella acicola and an unidentified member of Helotiales. Two basidiomycetes, Marasmius androsaceus and Mycena galopus, were also isolated relatively frequently from needles with snow cover, but under different temperature regimes. This preference for different temperatures reflects different niches that these species occupy in coniferous litter. Species richness was significantly higher on needles cultivated at lower temperatures. Widespread use of room temperature for cultivation of samples even from cold seasons may underestimate fungal diversity.
ABDULLAH S.K., MONFORT E., ASENSIO L., SALINAS J., LOPEZ LLORCA L.V., JANSSON H.B. (2010): Soil mycobiota of date palm plantations in Elche, SE Spain. [soil saprotrophic fungi, phytopathogenic fungi, Phoenix dactylifera, isolation methods] Czech Mycology 61(2): 149-162 (published: 10th August, 2010)
abstract
The mycobiota of soil from date palm (Phoenix dactylifera) plantations in Elche, SE Spain was examined using 23 soil samples and five isolation methods. One hundred and nineteen species assigned to 67 genera were isolated. The most frequent species were in decreasing order: Aspergillus fumigatus, A. niger, Neosartorya spinosa, Thielaviopsis punctulata, Chaetomium bostrychodes, Gilmaniella macrospora, Aspergillus candidus, Fusarium oxysporum, Rhizopus microsporus, Sordaria fimicola, Aspergillus terreus, Chaetomium murorum, Fusarium solani, Mucor racemosus, Penicillium citrinum and Thielaviopsis paradoxa. The thermotolerant and thermophilic species of Malbranchea cinnamomea, Myriococcum thermophilum, Rhizomucor miehei, Scytalidium thermophilum, Talaromyces emersonii, Thermoascus aurantiacus and Thermomyces lanuginosus were detected in various frequencies of occurrence. Our findings are compared with those from a similar survey of soil from date palm plantations in Iraq. Our study indicates that there is no characteristic mycobiota for soil in date palm plantations except for the more frequent isolation of some species well known as pathogens on date palm.
KOUKOL O. (2009): Geographical distribution of Scleroconidioma sphagnicola in coniferous forests in Europe and Canada. [Ascomycota, Larix decidua, Pinus spp., Picea spp., litter needles] Czech Mycology 61(1): 117-124 (published: 29th September, 2009)
abstract
The distribution of the dematiaceous microfungus Scleroconidioma sphagnicola in coniferous litter in Europe and Canada was assessed using a culture-dependent approach. Needles of various coniferous species were sampled between May and November of 2008. Twenty needles from each sample were cultivated on 2° malt agar after surface sterilisation with 30 % hydrogen peroxide. The formed mycelia were identified based on their morphology. The identifications were further confirmed by analyses of ITS rDNA. S. sphagnicola was recorded at 8 of the 53 sites. The highest abundance of S. sphagnicola was recorded in mountain areas of the Czech Republic. Further records of S. sphagnicola were acquired from the literature and the GenBank database. S. sphagnicola does not seem to be a ubiquitous coloniser of coniferous litter. However, this study showed that this fungus is found in various regions and the data obtained may serve to make an evaluation of the potential spread of S. sphagnicola.
JANKOWIAK R., ROSSA R., MIŚTA K. (2007): Survey of fungal species vectored by Ips cembrae to European larch trees in Raciborskie forests (Poland). [ophiostomatoid fungi, Ips cembrae, Larix decidua, fungi associated with bark beetles] Czech Mycology 59(2): 227-239 (published: 28th December, 2007)
abstract
The species composition of fungi associated with Ips cembrae was studied in the Raciborskie forests, Poland. The fungi were isolated from overwintered adults, larvae, new adults and from galleries at various stages of development. The results showed that there was a great diversity of fungi associated with this insect. We isolated 2877 cultures, including 61 species. The most important group of fungi were ophiostomatoid species. From these, Ceratocystis laricicola, Ophiostoma brunneo-ciliatum and Graphium laricis were commonly detected, whereas eight other species occurred less frequently or sporadically. Ophiostoma ips is documented here as a new associate of I. cembrae. Ceratocystis laricicola was shown to be the primary invader occurring most frequently at early stages of brood development, particularly in the sapwood. Ophiostoma brunneo-ciliatum and G. laricis were secondary invaders following C. laricicola. In the later stages of brood development other ophiostomatoid fungi appeared.
LYSKOVÁ P. (2007): Saprotrophic microscopic fungi and dermatophytes accompanying infections of the skin and nails of patients in the Moravian-Silesian Region (Czech Republic). [saprotrophic microscopic fungi, dermatophytes, superficial mycose, Scopulariopsis brevicaulis] Czech Mycology 59(1): 125-137 (published: 28th June, 2007)
abstract
Over a 19-month period, the spectrum of saprotrophic microscopic fungi isolated from 245 patients in the Moravian-Silesian Region (Czech Republic) was analysed. Saprotrophic microscopic fungi were isolated from nails (90 %) and skin (10 %). None was isolated from hair. The material was the most frequently positive for the presence of Scopulariopsis brevicaulis (32.6 %) followed by Cladosporium sphaerospermum (5.3 %), Aspergillus versicolor (4.0 %), Geomyces pannorum (4.0 %) and others.Dermatophytes and saprotrophic microscopic fungi were both studied within one year and represented 1110 isolates. Dermatophytes were isolated in most of the cases and represented 943 isolations (85 %). The saprotrophic microscopic fungus Scopulariopsis brevicaulis is a known causative agent of onychomycosis. In the evaluation including dermatophytes it ended in the 3rd position with 5.2 % of isolations behind Trichophyton rubrum (80 %) and T. mentagrophytes (8 %).
HASHEM M. (2004): Biological control of two phytopathogenic fungal species isolated from the rhizoplane of soybean (Glycine max). [biological control, soybean, Macrophominaphaseolina, Rhizoctonia solani] Czech Mycology 56(3-4): 223-238 (published: 22nd December, 2004)
abstract
Two hundred isolates representing 31 fungal species (20 genera) were recovered from soybean roots. Samples were collected from 12 localities at 3 different growth stages of the crop. The most dominant species were Aspergillus flavus, Fusarium oxysporum, Fusarium solani (Nectria haematococca), Macrophomina phaseolina and Rhizoctonia solani. Pathogenicity tests have proved the ability of Macrophomina phaseolina and Rhizoctonia solani to infect soybean roots and produce the symptoms of damping-off and root-rot diseases.The efficacy of three antagonists (Trichodermaharzianum, Epicoccum nigrum and Paecilomyceslilacinus) as well as two organic compounds (Strom and F-760) was evaluated as to their control of pathogenic fungi . Biocontrol fungi significantly suppressed Macrophominaphaseolina and Rhizoctonia solani in vitro and in vivo. Epicoccum nigrum and Paecilomyces lilacinus suppressed the growth of the pathogens by producing an inhibition zone while Trichoderma harzianum suppressed them by overgrowing. Strom and F-760 showed lower reduction effect of diseases in comparison with the antagonists.
FARGHALY R.M., GHERBAWY Y.A.M.H., YOSEF M.S. (2004): Contamination of meat stored in home refrigerators in Qena (Egypt). [microscopic fungi, stored meat, refrigerators, Aspergillus, RAPD-PCR] Czech Mycology 56(1-2): 53-62 (published: 12th August, 2004)
abstract
Eighty samples were collected from different parts of home-refrigerators and meat stored herein, in the province of Qena, Egypt. Quantitative and qualitative estimations of moulds were carried out by conventional methods and the identified Aspergillus spp. were confirmed by the RAPD-PCR technique in the Institute of Applied Microbiology (IAM), University of Agricultural Sciences, Vienna, Austria. The obtained results revealed that the highest mould count was 3.9 X 104 CFU/cm2 in the chest of there frigerators, followed by 3.2 X 10^4, 2.6 X 10^3 and 2.5 X 10^3 CFU/cm2 in samples of air and freezer of refrigerators and stored meat, respectively. Elevenmould genera could beidentified, the most common of which were Aspergillus, Penicillium and Cladosporium. The counts and relative frequencies for these genera were 31 (25.4 %), 17 (13.9 %) and 16 (13.1 %), respectively. Five Aspergillus species were identified; mainly A. flavus 13 (42.0 %), A. niger 5 (16.1 %) and A. nidulans 5 (16.1 %). The isolated Aspergillus species were subjected to further identification by r and om amplified polymorphic DNA (RAPD) by using type strains from IAM. RAPD-analysis indicated that the Aspergillus strains isolated during this study were completely identical with the corresponding type strains from IAM. Public health hazard and significance of mould contamination in home-refrigerators, as well as hygienic measures and recommendations are fully discussed to prevent or minimise such contamination.
GIRIVASAN K.P., SURYANARAYANAN T.S. (2004): Intact leaves as substrate for fungi: distribution of endophytes and phylloplane fungi in rattan palms. [phylloplane fungi, endophytes, Calamus, India] Czech Mycology 56(1-2): 33-43 (published: 12th August, 2004)
abstract
Leaves of twelve species of Calamus from southern India were screened simultaneously for the presence of phylloplane and endophytic fungi. Sampling of 2400 leaf segments yielded 824 endophyte isolates belonging to 34 species. Thirty species of phylloplane fungi were recorded. Several fungal species were found to be shared as endophytes by different hosts. However, the overlap between endophyte assemblage and phylloplane fungi of each host was low, suggesting that these two distinct groups of fungi occupy different niches, there by avoiding competition.
KUBÁTOVÁ A., KOLAŘÍK M., PRÁŠIL K., NOVOTNÝ D. (2004): Bark beetles and their galleries: well-known niches for little known fungi on the example of Geosmithia. [microfungi, Geosmithia, Scolytidae, ophiostomatoid fungi, yeasts] Czech Mycology 56(1-2): 1-18 (published: 12th August, 2004)
abstract
The oak bark beetle (Scolytus intricatus, Scolytidae, Coleoptera) was studied during the years 1997-2003 with respect to the occurrence of microscopic fungi on the surface of its body. Samples were collected in eight localities in the Czech and Slovak Republics. The investigation was focused on all different stages of the beetle’s life cycle: eggs, larvae, adults be for e emergence, adults in generation and maturation feeding (nearly 600 samples), and also on galleries (400 samples). The most frequent fungi associated with S. intricatus were yeasts, Geosmithia spp. and Penicillium spp. Ophiostomatoid fungi were isolated, too. Great attention was paid to the occurrence of Geosmithia spp., which were so far recorded rarely. They were frequently found in all stages of the life cycle of Scolytus intricatus, except for males in maturation feeding. The ecology of Geosmithia spp. in feedings of phloem inhabiting insects is discussed for their negative cellulase production and the ecology of associated insect species. Trees infested with Scolytus intricatus represent a major and still little explored niche of Geosmithia spp.
MARIA G.L., SRIDHAR K.R. (2003): Endophytic fungal assemblage of two halophytes from west coast mangrove habitats, India. [mangroves, halophytes, endophytes, fungi, India] Czech Mycology 55(3-4): 241-251 (published: 22nd December, 2003)
abstract
Twenty-five endophytic fungi comprising three ascomycetes, 20 mitosporic fungi and two sterile fungi were recovered from two halophytes (Acanthus ilicifolius and Acrostichum aureum) of a west coast mangrove habitat in India. Overall colonisation of tissue segments by endophytes ranged between 74.5 % (Acanthus ilicifolius) and 77.5 % (Acrostichum aureum). Analysis using the Jaccard’s similarity coefficient revealed 16-25 % similarity in endophyte assemblage among different tissues, and 24.5 % between the two hosts. Out of four tissues screened, species richness and diversity were high in stems of Acanthus ilicifolius and roots of Acrostichum aureum. The most dominant endophyte was Colletotrichum sp. in prop roots of Acanthus ilicifolius, and Yeast sp. 1 in rhizomes of Acrostichum aureum. Among the dominant endophytes (colonisation frequency >5 %), Acremonium and Yeast sp. 1 were common to both hosts. Acanthus ilicifolius showed dominance of a single species, (Colletotrichum sp.), while in Acrostichum aureum multiple species dominance was seen (Acremonium sp., Pénicillium sp. and Yeast sp. 1). Only one typical marine mitosporic fungus (Cumulospora marina) was recovered from the roots of Acanthus ilicifolius.
Colloquium "Fungi as Model Organisms in Research and Biotechnology - II", Olomouc, Czech Republic, September 5th–6th, 2002. Czech Mycology 55(1-2): 103-149 (published: 23rd July, 2003)
abstract
The colloquium was a continuation of a previous scientific meeting that took place in Olomouc in 1999 (Czech Mycology 52: 139-178, 2000). It was organised by the joint Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology together with the Institute of Biology, Faculty of Medicine of Palacký University, Olomouc. The purpose of the colloquium was to provide a platform for a broad discussion on the use of fungi as model organisms in both basic and applied research. The programme of the colloquium was divided into four parts dealing with the following topics: biochemistry, biotechnology and genetics of fungi; phytopathogenic fungi; fungi pathogenic to humans and animals; and mycology of food and mycotoxins. Each topic was opened with a plenary lecture (30 min.), followed by short communications (10 min.) and accompanied by poster presentations. Besides five plenary lectures, 20 short communications and 24 posters were presented. In total 42 researchers took part in the colloquium and discussed various topics important for the further direction of experimental mycology. Abstracts of the contributions are given here.
NOVÁKOVÁ A., PIŽL V. (2003): Mycoflora in the intestine of Eisenia andrei (Oligochaeta, Lumbricidae) and in vermiculture substrates. [cattle manure, saprotrophic and cellulolytic microfungi, earthworms] Czech Mycology 55(1-2): 83-102 (published: 23rd July, 2003)
abstract
Mycoflora of three commercial vermiculture systems based on cattle manure derived sub strates and Eisenia andrei earthworms was studied using several isolation methods. A total of 172 taxa of saprotrophic micromycetes were isolated (19 taxa of Zygomycetes, 9 taxa of Ascomycetes and 144 taxa of mitosporic fungi). Aspergillus fumigatus was the most frequent microfungal species in the intestine of Eisenia andrei. In vermicuture substrates, Aspergillus flavus and Aspergillus fumigatus were among species isolated very frequently by the soil dilution method, while Rhizopus stolonifer was estimated as frequent species using the soil washing isolation technique.
NOVOTNÝ D. (2003): A comparison of two methods for the study of microscopic fungi associated with oak roots. [Quercus robur, oakdecline, Czech Republic, mycobiota, ophiostomatoid fungi] Czech Mycology 55(1-2): 73-82 (published: 23rd July, 2003)
abstract
Roots of four trees with symptoms of oak decline and roots of one healthy tree of Quercus robur were examined for the presence of fungi by using two methods (moist chambe rmethod and strong surface sterilisation method). Forty-five species were isolated in this project. Significant differences in composition of mycobiota based on the used method were detected. Fusarium solani, F. proliferatum, Sphaerostilbella aureonitens, Cylindrocarpon destructans, Pénicillium simplicissimum, P. purpurogenumvar. rubrisclerotium, Trichodermaviride, Ophiostomapiceae s.l. and Pénicillium glandicola were the most frequent fungi isolated by the moist chamber method. Cryptosporiopsis radicicola, dark sterile mycelium sp. 1, Cylindrocarpon destructans, ChaetomiuM globosum, Cylindrocarpon didymum, Pénicillium simplicissimum and Trichoderma koningii were dominant species observed by the method of strong surface sterilisation.
NOVOTNÝ D. (2002): Contribution to the knowledge of the mycoflora in roots of oaks with and without tracheomycotic symptoms. [Quercus robur, Q. petraea, Q. rubra, oak decline, ophiostomatoid fungi, Penicillium, Czech Republic, Moravia] Czech Mycology 53(3): 211-222 (published: 10th January, 2002)
abstract
The mycoflora of roots of three species of oak (Quercus robur, Q. petraea, Q. rubra) from two localities near Moravské Budějovice (southwest Moravia, Czech Republic) with and without tracheomycotic symptoms was studied. Fifty-seven species of fungi were identified from the samples. The most frequently isolated were Fusarium solani, Penicillium gl and icola, P. glabrum, P. simplicissimum and Acremonium curvulum. In the roots of trees with tracheomycotic symptoms two species of ophiostomatoid fungi were recorded. Abiotic condiontions (insufieciency of precipitation and higher average temperature) are considered to be primary reasons of oak decline.
HÝSEK J., BROŽOVÁ J. (2001): The role of some saprophytic micromycetes and fungus Micromucor ramannianus var. ramannianus in forest soil. [humic horizon, soil fungi, saprophytic micromycetes, Micromucorramannianusvar. ramannianus, biological soil functions, respiration, ammonification, nitrification] Czech Mycology 53(2): 161-171 (published: 20th February, 2002)
abstract
Different saprophytic micromycetes were isolated from the humic horizon (H-A 02) of different types of forest soils (barren l and of reforested waste dumps, cambisol of spruce, birch, European mountain ash, and blue spruce forests) in several areas (at Most in the Krušné hory (Ore Mts.), Jizerské hory (Izera Mts.)). Besides the spectrum of common species of soil micromycetes (Penicillium spp., Humicola spp., Trichoderma spp., Paecilomyces spp., Scopulariopsis spp., Aureobasidium spp., Mucor spp., Absidia spp.), the fungus Micromucor ramannianus (Möller) Arx var. ramannianus (Mortierella ramanniana (Möller) Linneman, Mucor ramannianus Möller) was regularly isolated from all types of soils, except barren soils of was te dumps. The biological quality of forest soils in connection with other biological characteristics was evaluated in relation to the presence and quantity of this fungus in forest soils. Basic biological processes (basal and potential respiration, ammonification, nitrification) show an increased intensity in forest soils in which the proportion of Micromucorramannianus v. ramannianus was not present in the soil of the worst biological quality (lower values of biological soil parameters), e.g. in of was te dumps. It is a topic for discussion whe the r this fungus can also be an indicator of environmental pollution.
MOSTAFA M.E., ZOHRI A.A., KOTBY R.S. (2001): Lipid, sterol and ergosterol accumulation in isolates of dematiaceous Hyphomycetes. [Lipid, sterol, ergosterol, dematiaceous hyphomycetes] Czech Mycology 53(2): 149-159 (published: 20th February, 2002)
abstract
Mycelial dry weight, lipid and sterol contents of fungi tested varied with fungalgenus, species and even with isolate of one species. Their dry mass fluctuated between lll.6±l0.7-457.0±41.5 mg/50 ml medium. Lipids, sterols and ergosterol accumulated by the isolates tested ranged from 4.52±0.5-29.04±2.76%, 1.23±0.16-10.63±l.24% and 0.43±0.057-7.13±0.695% of their dry mass, respectively. Cochliobolus spicifer isolate No. 35 was the highest lipid-producer while Ulocladium atrum No. 90 proved to be superior in the production of sterols and ergosterol. TLC technique and chemical analysis of lipid classes produced by U. atrium No. 90 revealed that the lipid fractions are composed of free sterols, free fatty acids, sterol esters, glycolipids, phospholipids and squalene.
GHERBAWY Y.A.M.H., PRILLINGER H. (2000): Root mycoflora of pepper (Capsicum annuum) antagonistic to Verticillium dahliae. [Frequency of root fungi, Verticillium dahliae, Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, Trichoderma harzianum, pepper seed germination] Czech Mycology 52(3): 219-226 (published: 7th December, 2000)
abstract
Thirty-two species belonging to 19 genera of fungi were collected from 30 soil samples from the rhizosphere of pepper plants. The fungal colonies were characterised using classical morphological methods following identification keys. The most frequently isolated fungi were Chaetomiumglobosum, Fusarium oxysporum, Gliocladium roseum, Mucor racemosus, Myrothecium verrucaria, Penicillium aurantiogriseum, P. expansum and Trichoderma harzianum. The crude culture filtrate of Verticillium dahliae at 100% concentration caused sharp decrease in pepper seed germination. Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, and Trichoderma harzianum produced a metabolite that retarded radial growth of Verticillium dahliae.
Colloquium "Fungi as Model Organisms in Research and Biotechnology", Olomouc, September 14th, 1999. Czech Mycology 52(2): 139-178 (published: 20th April, 2000)
abstract
The colloquium was organized by the joint Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology. The purpose of the colloquium was to provide a platform for a broad discussion on the use of fungi as model organisms in both basic and applied research. The programme of the colloquium was divided into four parts dealing with the following topics: phytopathology, biotechnology and ecology, physiology and biochemistry, and medical mycology. Each topic was opened with a plenary lecture (30 min.), followed by short communications (10 min.) and accompanied by poster presentations. Besides four plenary lectures, 15 short communications and 21 posters were presented. In total 38 researchers took part in the colloquium, at who discussed various questions of importance for experimental mycology until late in the evening. Abstracts of the contributions are given here.
GHERBAWY Y.A.M.H., ABDELZAHER H.M.A. (1999): Isolation of fungi from tomato rhizosphere and evaluation of the effect of some fungicides and biological agents on the production of cellulase enzymes by Nectria haematococca and Pythium ultimum var. ultimum. [Biologicalcontrol, root-rot, fungicides Nectria haematococca, Pythium ultimum var. ultimum] Czech Mycology 51(2-3): 157-170 (published: 25th May, 1999)
abstract
Forty-five species and two species varieties belonging to twenty-six genera of fungi were isolated from 30 soil samples from the rhizosphere of tomato plants. The fungi most frequently isolated were Aspergillus flavus, A.fumigatus, A. niger, A. terreus, Gibberella fujikuroi, Nectria haematococca and Rhizopus stolonifer. Ridomil and Vitavax-captan (10, 50 and 100ppm) had no significant effects on the activity of Cl and Cₓ enzymes of Nectria haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but slightly decreased at 100ppm. Vitavax-captan (10, 50 and 100ppm) significantly decreased Cl enzyme activities of N. haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but at 100 ppm it showed a slightly inhibitory effect. Ridomil caused a slight increase in the activity of Cₓ and Cl enzymes by Pythium ultimum var. ultimum at low and moderate doses but the highest dose of Ridomil caused a slight reduction. Vitavax-captan slightly increased the activity of Cₓ and Cl enzymes in P. ultimum var. ultimum. Normal and sterilised filtrates of Myrothecium verrucaria, Penicillium oxalicum and Trichoderma harzianum induced a small decrease in Cl enzyme activity of Nectria haematococca. The sterilised filtrates of the three fungi tested caused greater inhibition compared to the normal filtrate. The production of Cₓ enzyme was slightly increased with normal and sterilised filtrates of Penicillium oxalicum and Trichoderma harzianum, but was significantly increased by both types of filtrates of Myrothecium verrucaria. The two types of filtrate of all fungi tested did not significantly affect the activity of Cl and Cₓ enzymes by lithium ultimum var. ultimum. Production of extracellular protein by Nectria haematococca was not significantly affected by any dose of the tested fungi cides. It was slightly increased by the two types of filtrate of the three tested fungi but significantly increased by the normal filtrate of Myrothecium verrucaria. The normal filtrate of all the fungi tested enhanced extracellular protein production to a greater extent than the sterilised filtrate. Extracellular proteins of Pythium ultimum var. ultimum were slightly increased by all doses of Vitavax-captan and low doses only of Ridomil, also two types of filtrate of all tested fungi caused a slightly increasing effect.
HUŇKOVÁ Z., KUBÁTOVÁ A., WEIGNEROVÁ L., KŘEN V. (1999): Induction of extracellular glycosidases in filamentous fungi and their potential use in chemotaxonomy. [glycosidases, induction, Aspergillus, Penicillium, Fusarium] Czech Mycology 51(1): 71-87 (published: 29th January, 1999)
abstract
Data on the occurrence and inducibility of extracellular β-N-acetylhexosaminidase, α-galactosidase, α- and β-mannosidase and α-L-fucosidase, including inductors, are given for selected Aspergillus, Penicillium and Fusarium strains. These data represent additional information on the strains in the Culture Collection of Fungi, Department of Botany, Charles University, Prague, and in the Culture Collection of the Institute of Microbiology, Prague, Czech Republic, thus extending their usability in biochemistry and biotechnology. With respect to these biochemical data a taxonomic evaluation of the examined strains is presented. Several strains were re identified after biochemical and morphological comparisons with the type strains. The strains of A. niveus CCF 544, A. terreus CCF 76, CCF 869, and CCIM USA were re-identified as A. flavipes, the strain A. oryzae CCF 1301 as A. wentii.
REINPRECHT L. (1998): Loss of antifungal activity of selected fungicides in treated wood due to natural ageing. Part 1: Activity against moulds. [2-thiocyanomethylthiobenzothiazole, organotin fungicides, beechwood, natural ageing, moulds] Czech Mycology 50(4): 249-258 (published: 12th July, 1998)
abstract
The activity of 2-thiocyanomethylthiobenzothiazole (TCMTB) and selected organotin com pounds (OTC = TBTO, TBTS, TBTCA and TBT-DEDTK) against moulds was evaluated by means of mycologicaltestsin which treated and subsequently naturally aged beechwood samples were exposed to the effect of a mixture of six moulds under laboratory conditions. Natural ageing of the treated samples took place under an angle of 45’, and during periods of 0, 2 or 4 months. TCMTB was characterized as the relatively most stable fungicide with antimould activity.
ABDEL-MALLEK A.Y., MAZEN M.B., ALLAM A.D., HASHEM M. (1997): Specific responses of some phytopathogenic fungi to fungicides. [fungicides, phytopathogenic species, Egypt] Czech Mycology 50(1): 35-44 (published: 29th September, 1997)
abstract
Laboratory experiments were carried out to examine the effect of four fungicides on spore germinating potentialities, radial growth and survival of viable propagules in soil of five phytopathogenic fungal species. The test organisms were achieved from infected roots of wheat plants cultivated in the Assiut area, Egypt. These were: Altemaria alternata, Cochliobolus sativus, Drechslera halodes, Fusarium moniliforme and F. oxysporum. The fungicides reduced germ tube production and radial growth of all fungi, and the reduction increased with increase in concentration. The maximal reduction was recorded at 50 μg/ml. At this concentration, Homai prevented spore germination of all test species. Neither F. moniliforme nor F. oxysporum can grow on agar medium supplemented with 50 μg/ml of either Benlate or Homai. The suppressive effect of fungicides on spore survival in soil cultures was also noticed but seemed to be lower than in agar application. In certain treatments, the numbers of viable propagules of tested fungi were not significantly affected in autoclaved nor non-autoclaved soil.
FASSATIOVÁ O. (1995): Micromycetes in archives and book depositories in the Czech Republic. [Micromycetes, archives, allergies, Czech Republic] Czech Mycology 48(1): 77-86 (published: 16th May, 1995)
abstract
Species representation of micromycetes and their frequency were studied in the period 1981-1988 in four archives in Prague and near Prague and in one depository in West Bohemia. Representants of genera Penicillium, Cladosporium, Alternaria, Aspergillus, Mucor and Rhizopus isolated by sediment plate method and from the surfaces of the archive depots were the most frequent in all observed spaces. The higher number of the most frequent species was always found in older and for the archive purposes less convenient buildings, while inside the new building the number of the most frequent species was very low. In suitable conditions only a limited number of species for which the given specific conditions are convenient, act as destructive. They are mostly Penicillium which form coherent growing covers on the backs of the books and cartons. Spores of these fungi released by their growth contaminate the atmosphere of these spaces and can cause allergies in sensitive persons.
MOLITORIS H.P. (1995): Fungi in biotechnology. Past, present, future. [Fungi, biotechnology, past, present, future] Czech Mycology 48(1): 53-65 (published: 16th May, 1995)
abstract
From multiple uses of fungi in early historic times, “early biotechnological processes” developed. Fungi were used here already e.g. for production of cheese, bread, wine, beer and other foodstuff. Based on these processes, fungi today have become one of the most important group of organisms in modern technology, where food, fodder and various metabolites such as antibiotics, enzymes, steroids etc. are produced on an industrial scale. An equally important role of fungi in biotechnology is also to be expected for the future where - in addition to existing uses - fungi will be increasingly used employing modern methods such as genetic engineering and will also new applications as in pollution control, biological control of pests, microbial leaching and even biotechnology.
PIECKOVÁ E., JESENSKÁ Z. (1994): The effect of the heat-stable and chloroform-extractable secondary metabolites of filamentous fungi on the respiratory tract cilia movement of one-day-old chickens in vitro. [Fungi, cilia, trachea, chickens, metabolites, bronchitis] Czech Mycology 47(3): 215-221 (published: 17th June, 1994)
abstract
The ciliostatic activity of the heat-stable (100°C/10 min.) and chloroform-extractable metabolites of 63 strains of filamentous fungi - growing on the liquid medium - on tracheal cilia of one-day-old chickens in vitro was evaluated. Twenty two (34.9%) from the investigated strains produced ciliostatic metabolites, 4.7%, 7.9%, 3.1%, resp. 19.0% of the strains stopped the movement of cilia after 24, 48, 72, resp. 144 hours. The results are discussed in connection with chronic bronchitis of people working with moulded materials or living in moulded dwellings.
KUBÁTOVÁ A. (1994): New records of micromycetes from Czech and Slovak Republics. III. Acremonium furcatum, Gonatobotryum parasiticum, Stachybotrys bisbyi, and Wardomyces inflatus. [Filamentous microfungi, Deuteromycotina, new records, Czech Republic, Slovak Republic] Czech Mycology 47(2): 151-158 (published: 15th March, 1994)
abstract
Four species of the lesser known filamentous microfungi (Deuteromycotina) are reported from Czech and Slovak Republic, which appear to be the first published records of these fungi for this area. Acremonium furcatum, Stachybotrys bisbyi and Wardomyces inflatus were isolated from soil, Gonatobotryum parasiticum was found on Trichoderma sp. on dead wood. Description and illustrations are given. The strains of the three former fungi are maintained in the Culture Collection of Fungi (CCF), Prague.
NOVÁKOVÁ-ŘEPOVÁ A., CHALUPSKÝ J. (1993): Microscopic fungi isolated from Fridericia galba (Oligochaeta, Enchytraeidae). Česká Mykologie 46(3-4): 251-255 (published: 25th August, 1993)
abstract
Microscopic fungi were isolated from the enchytraeid Fridericia galba (Hoffmeister, 1843), and from surrounding soil. Isolation was done from aseptically squashed enchytraeid bodies and from enchytraeid excrements prepared by defaecation into sterile conditions. The cultivation media were soil extract agar, cherry decoction agar and beer wort agar. A total of 39 fungal species was found, 29 in the excrements and 19 in the soil. In most cases, fungi isolated from the soil differed from those isolated from the excrements. Feeding preference and seasonal variation were not demonstrated. Spores of fungi were viable after passage through the enchytraeid gut. The biochemical activity of the isolated fungi indicates preferential feeding on plant remains where the degradation of cellulose, pectin and xylose predominates.
HOLEC J. (1993): Ecology of macrofungi in the beech woods of the Šumava mountains and Šumava foothills. Česká Mykologie 46(3-4): 163-202 (published: 25th August, 1993)
abstract
The fungi of the order Agaricales s. l. and several families of ungilled fungi and gasteromycetes were studied in the beech woods of the southeastern part of the Sumava mountains and Sumava foothills (Czechoslovakia). Altogether, 230 species were recorded on 8 permanent plots (50 x 50 m) during the years 1988 - 1990. The terrestrial fungi were closely associated with a particular layer of the surface humus and substrate, and the lignicolous fungi were associated with wood in various stages of decay. The occurrence of mycorrhizal fungi was influenced above all by the mycorrhizal partner, altitude, and climate. The species composition, number of mycorrhizal and terrestrial species on the individual plots, and their share were determined by the humus type, microrelief, and the thickness of the detritus layer. The occurrence of lignicolous fungi was in close relation to the degree of naturalness of the wood, substrate diversity of the plot and the mesoclimate. The results are summarized in the mycosociological tables and compared by the use of cluster analysis and diagrams.
ŠIMONOVIČOVÁ A. (1992): Microfungal community structure in forest soils of North Slovakia. II. Similarity of mykocenosis (Krížna, Koprová and Furkotská Vallies). Česká Mykologie 46(1-2): 106-113 (published: 1st April, 1993)
abstract
On five localities in vallies Krížna, Koprová and Furkotská (High Tatra Mts.) we investigated the species structure of soil microscopic fungi and the similarity of mycocenosis. The arenic podzol and podzolic ranker soils were studied. The soils had low to very low acid reaction and very low content of organic matter. In the global number of isolated soil microscopic fungi species (48), the cosmopolitan genus Penicillium of the family Moniliaceae was dominantly represented. The species of the family Mucoraceae were suppressed as the result of the soil quality decreasing, which reflects the degradation of the quality of the whole environment in High Tatra Mts. The highest index of similarity – S = 0.6 – was proved between the localities in the neighboring vallies.
ŠIMONOVIČOVÁ A. (1992): Microfungal community structure in forest soil of North Slovakia. I. Similarity of mykocenosis (The Valley Tichá). Česká Mykologie 46(1-2): 99-105 (published: 1st April, 1993)
abstract
Microfungal community structure in four forest localities in the Valley Tichá in the High Tatra Mountains was studied. Podzolic and ranker soils are poor in organic matter, physically and chemically unfavourable. The pH values are lower – by 1.0 to 1.5 – than values in 1962, which means that the quality of the soils has degraded. These differences correspond with the quality of mycocenosis, which has also worsened. 41 species of soil micromycetes were isolated. The occurrence of the representatives of the genus Penicillium (fam. Moniliaceae) was the highest. The occurrence of the representatives of the family Mucoraceae was suppressed. The similarity of the neighbouring mycocenoses was higher than that of those more distant from each other.
NOVÁKOVÁ-ŘEPOVÁ A. (1992): The growth of soil micromycetes in the media containing herbicides Basagran, Labuctril 25 and Oxytril CM. Česká Mykologie 46(1-2): 86-92 (published: 1st April, 1993)
abstract
The changes of fungal growth in laboratory experiments (estimation of mycelial biomass and colony diameter) were studied by cultivation of 10 species of soil fungi on media with different concentrations of the herbicides Basagran, Labuctril 25 and Oxytril CM. The subject fungi responded to increased concentrations of herbicide in different ways. Cladosporium herbarum (colony diameter) and Penicillium janthinellum (mycelial biomass) were the only investigations to show a similar response to all the herbicides. Penicillium janthinellum (mycelial biomass) was the only example of a significant stimulation of fungal growth. Changes in colony pigmentation and sporulation were also recorded.
KUTHAN J. (1992): Mycoflora of large-scale greenhouse plantation of salad cucumbers. Česká Mykologie 46(1-2): 1-32 (published: 1st April, 1993)
abstract
In the course of the years 1988–1991 the author used to visit large-scale greenhouses at Paskov near Ostrava (NE Moravia, Czechoslovakia) and surveyed not only the abundant growth of particularly higher fungi there, but in 1989 he tried to summarize the weight of biomass of the most frequent species. The results of the observation of biomass, frequency, abundance and phenology are presented in the tables. Altogether 95 macrofungi taxons, 1 species of Hyphomycetes and 1 species of Myxomycetes were recorded and they are given in the systematic summary, potentially with comments.
JANČAŘÍK V. (1991): Ing. Hana Červinková, CSc. sexagenaria. Česká Mykologie 45(1-2): 59-62 (published: 15th May, 1991)
URBAN Z. (1991): Abstracts of papers delivered at the 8th conference of Czechoslovak mycologists held in Brno (August 28-September 1. 1989). Česká Mykologie 45(1-2): 33-53 (published: 15th May, 1991)
abstract
Abstracts of papers delivered at the 8th conference of Czechoslovak mycologists held in Brno August 28 — September 1,1989)
VONDROVÁ S. (1991): Echinostelium minutum and other Myxomycetes developed in moist chamber culture. Česká Mykologie 45(1-2): 27-32 (published: 15th May, 1991)
abstract
Using the method of moist chamber culture some species of Myxomycetes on plant material were developed: Arcyria cinerea, Badhamia sp., Comatricha jimbriata, Didymium squamulosum, Echinostelium minutum and Enerthenema papillatum.
ŘEPOVÁ A. (1990): Soil micromycetes from from Czechoslovakia - a list of isolated species with bibliography. IV. Česká Mykologie 44(3): 170-178 (published: 22nd October, 1990)
abstract
A list of micromycetes (including saprophytic, keratinophilic, rhizosphere, nematophagous, ovicidal, dermatophytic fungi, and cellular slime moulds) isolated from Czechoslovak soils is presented with references and data on their distribution. Listed species include Talaromyces flavus, T. luteus, T. purpureus, T. trachyspermus, T. wortmanii, Tetracoccosporium paxianum, Thamnidium elegans, Th. verticillatum, Thielavia basicola, Th. terricola, Thumenella sp.
KUBÁTOVÁ A. (1989): Eladia saccula (Dale) G. Smith, a new micromycete for Czechoslovakia. Česká Mykologie 44(1): 20-25 (published: 22nd February, 1990)
abstract
Two strains of the rather rare micromycete Eladia saccula (Dale) G. Smith were isolated from forest soil in Czechoslovakia and Poland. They are maintained in the Culture Collection of Fungi, Department of Botany, Charles University, Prague (CCF 1978 and CCF 2391). Eladia saccula is reported from Czechoslovakia for the first time. A description is provided on several nutrient media (CZ, CYA, MEA, G25N, and beer wort agar) at 25 °C, 5 °C and 37 °C. The ability to grow at 37 °C is remarkable. Growth is reduced on medium with limited water availability (G25N). Based on typical conidiophore and phialide structures, placement in the genus Eladia is supported over Penicillium.
FASSATIOVÁ O., KUBÁTOVÁ A., PRÁŠIL K., VÁŇOVÁ M. (1987): Microscopical fungi in archive environment. Česká Mykologie 41(1): 8-15 (published: 16th February, 1987)
abstract
One new genus, Amicodisca (typified by Dasyscypha brdensis Vel.) and six new species are described: Albotricha lupini, Ciliolarina corcontica, Cystopezizella cupulincola, Hamatocanthoscypha acericola, Hyaloscypha cupularum, and Orbilia ebuli. One new combination (Phaeohelotium terrestre) is proposed.
RYPÁČEK V. (1986): Some interesting ways of wood infection by fungi. Česká Mykologie 40(4): 193-202 (published: 7th November, 1986)
abstract
Infections caused by wood-decay fungi proceed in various ways depending not only on the fungal species but also on the substrate's chemical and physical properties. Spores released from hymenia may reach hundreds of thousands per hour per square decimeter, attacking both living and dead wood. Successful colonization is influenced by microbial antagonists like Trichoderma viride or Bacillus asterosporus. Spores may lose viability within days if they fail to germinate. In trees, wounds allow entry, and anatomical structure affects spore migration.
Seminar Fungi in Biotechnology, Brno 1985 (Šašek V.). Česká Mykologie 40(2): 110-115 (published: 10th May, 1986)
BETINA V. (1986): Fungi in biotechnology-history, present times, perspectives. Česká Mykologie 40(2): 65-73 (published: 10th May, 1986)
abstract
A review of applications of fungi in biotechnology from the old ages to the present times is presented. Origins of vine and beer fermentation as well as leavened bread making are lost in the mist of antiquity and records of the production of Roquefort cheese go back about a thousand years. On the other hand, commercial production of yeast began in the last century and the production of single-cell proteins (SCP) was born in our century. Nowadays, fungi are further used in commercial production of enzymes, organic acids, vitamins, antibiotics and plant growth regulators, in biotransformations of steroids and antibiotics, in the ephedrin synthesis and in preparation of other pharmacologically useful substances. Great achievements were obtained by applying classical genetic methods. Possibilities of the use of protoplast fusion and recombination, gene amplification or recombinant DNA technology using restriction enzymes are discussed.
Seminar on „Morphogenesis of Fungi“, Olomouc, May 31, 1984. Česká Mykologie 40(1): 52-58 (published: 7th February, 1986)
ŘEPOVÁ A. (1986): The occurrence of microscopic fungi in air of the building of the Czechoslovak Acadeny of Sciences in České Budějovice. Česká Mykologie 40(1): 19-29 (published: 7th February, 1986)
abstract
68 species of microscopic fungi were isolated from the air in the building of the Czechoslovak Academy of Sciences in České Budějovice. 16 species were opportunistic, 12 allergenic, and 13 mycotoxin producers. 8 species were reported as air-borne fungi in literature.
ROD J. (1984): Antagonistic effects of some fungi on fungal pathogens causing storage rots of onion (Allium cepa L.). Česká Mykologie 38(4): 235-239 (published: 1984)
abstract
14 species of soil micromycetes belonging to Fungi imperfecti isolated from soil of spruce and mixed forests, apple orchard, uncultivated balk and Salix–Populus community are presented as new finds in Czechoslovakia.
ONDŘEJ M. (1984): Funde von parasitischen imperfekten Pilzen Cercospora Fres. Aus der Tschechoslowakei (Teil III.). Česká Mykologie 38(4): 230-234 (published: 1984)
abstract
The fungi Gliocladium roseum, Trichothecium roseum, Trichoderma harzianum, Trichoderma viride, Penicillium cyclopium and Acremoniella atra were isolated from onion bulbs infected with parasitic fungi Botrytis allii, Botrytis cinerea, Botrytis squamosa, Sclerotium cepivorum and Fusarium oxysporum. Some of them exhibited antagonistic effects on the pathogens causing storage rots under in vitro and in vivo conditions.
STANĚK M. (1984): Microorganisms in the hyphosphere of fungi. I. Introduction. Česká Mykologie 38(1): 1-10 (published: 10th February, 1984)
abstract
The surface and immediate surroundings of fungal hyphae affected by their activity is termed the „hyphosphere“. Differences between microorganisms colonizing the hyphosphere and those colonizing a substrate is termed „hyphosphere effect“. The more extensive area of fungal effect on substrate, on the surface and surroundings of fruit-bodies, i.e. the environment affected by the activity of fungi is termed „mycosphere“. The nomenclature proposed is in agreement with the classification of the phytosphere of higher plants. The brief literary survey presents data on incidence of microorganisms in hyphosphere and on their importance, taking into account the results obtained in the Czechoslovak research institutions.
Abstracts of papers delivered at the 7th Conference of Czechoslovak mycologists held at České Budějovice, 13-18 September, 1982. Česká Mykologie 37(2): 108-128 (published: 1983)
abstract
Abstracts of papers delivered at the 7th Conference of Czechoslovak Mycologists, held at České Budějovice, 13–18 September 1982 - 1st Section: Taxonomy, Ecology and Mycofloristics
ŘEPOVÁ A. (1983): Soil micromycetes of forest reserve „Voděradské bučiny“ in Central Bohemia. Česká Mykologie 37(1): 19-34 (published: 1983)
abstract
In three forest sites of the Nature reserve “Voděradské bučiny” soil microfungi were studied in the period from September 1978 to July 1979. A total of 66 species was identified, 9 of which belonged to Mucoraceae and 57 to Moniliales. The most common species were Penicillium albidum Sopp emend. Fass. (all sites) and Trichoderma viride Pers. ex S. F. Gray (beech forest, beech forest with Dicranum), Absidia cylindrospora Hagem (beech forest), P. spinulosum Thom (beech forest with Dicranum, spruce forest), P. chrysogenum Samson, Hadlok et Stolk (beech forest, spruce forest). The greatest number of species was found in the spruce forest. The highest biomass values of mycelium and of spores were ascertained in stony soil of the beech forest with Dicranum scoparium.
ČAČA Z. (1982): Present trends in the world phytopathological research. Česká Mykologie 36(2): 65-71 (published: 15th May, 1982)
abstract
Vararia granulosa (Fr.) Laurila is a name which cannot be applied to the fungus for which it is now commonly used. Fries (1838, 1874) considered the element representing our Vararia only a marginal part of his broad concept of Grandinia granulosa (Pers. ex Fr.) Fr. As there is no legitimate name for the fungus in question, Vararia borealis Pouz. spec. nov. is proposed for it here.
ZAJÍCOVÁ S., JABLONSKÝ I., JAŠA B. (1981): The influence of substratum composition and cultivating method on fruit-body yield in Flammulina velutipes (Curt. Ex Fr.) Sing. Česká Mykologie 35(3): 152-160 (published: 1981)
abstract
In studies of the influence of optimal substratum composition on mycelia growth and fruit-body formation in Flammulina velutipes it was found out: the highest lineal daily mycelia growth rate was achieved on cultivating media from wheat straw (0.51 mm) and shredded corn cobs (0.50 mm). Cultivated on unsupplemented media the best yields were achieved on corn cobs (20.97%) and wheat straw (19.13%). The best yields were obtained from F. velutipes cultivated on corn cobs supplemented by a mixture of 10% bone meal and 12% wheat corn meal (28.4%). Evaluating the influence of different methods of cultivation on yield the following was found out: the optimal duration of media incubation by mycelia in supplemented corn cobs was 21 days, whereas in unsupplemented mixture sawdust:straw:corn cobs (ratio 1:1:1) the best yield was achieved after 35 days of incubation. By the reduction of the yielding surface by 2/3 a substantial increase in the average weight of fruit-bodies was obtained with a slight drop of yield as compared with plots with unlimited yielding surface. Sterilized media gave a yield which was higher by 1% than pasteurized ones.
HOLUBOVÁ-JECHOVÁ V. (1979): Lignicolous and some other saprophytic Hyphomycetes from Hungary. Česká Mykologie 33(3): 138-149 (published: 1979)
abstract
Fifty nine species or varieties of lignicolous and other saprophytic Hyphomycetes belonging to 41 genera are recorded from Hungary. The majority of them are common species, occurring in Europe, however, previously unreported from Hungary. Some of rare and infrequent lignicolous Hyphomycetes were also collected.
HUBÁLEK Z., ROSICKÝ B., OTČENÁŠEK M. (1979): Fungi on the hair of small wild mammals in Czechoslovakia and Yugoslavia. Česká Mykologie 33(2): 81-93 (published: 1979)
abstract
A new record of dry rot fungus Serpula lacrimans (Wulf. ex Fr.) Schroet. on the trunk bases of two living trees of Norway Spruce Picea abies (L.) Karst. in the Botanical Garden in Průhonice near Prague is described. Differences between Serpula lacrimans and Serpula himantioides (Fr. ex Fr.) Karst. and the phytopathological significance of the new record are discussed.
Varia. Česká Mykologie 33(1): 60-61 (published: 1979)
FASSATIOVÁ O. (1978): Soil micromycetes in abandoned fields in Bohemian Karst. Česká Mykologie 32(4): 226-234 (published: 1978)
abstract
An analysis of soil micromycetes in two abandoned fields in the Bohemian Karst during 1976–1977 revealed 64 species in the younger field (7 years) and 50 in the older (50 years). Dominant species in both were: Penicillium albidum, Fusarium solani, Humicola grisea, Absidia cylindrospora, and Mortierella sp. (section Polycephala). The best isolation method used nylon gauze buried in soil.
Abstracts of papers delivered at the 6th Conference of Czechoslovak mycologists held at Pezinok, 19.-23. September 1977. Česká Mykologie 32(2): 99-122 (published: 1978)
abstract
Tradice mezinárodních mykologických kongresů není stará. První byl v roce 1971 v anglickém Exeteru. K vrcholným vědeckým setkáním tohoto typu nelze přiřazovat již delší dobu pořádané Sjezdy evropských mykologů. Druhý mezinárodní mykologický kongres se konal ve dnech 27. VIII. až 3. IX. 1977 v prostorách University of South Florida v Tampě. Zúčastnilo se ho více než 1200 specialistů ze 43 zemí.
JANEČKOVÁ V., FASSATIOVÁ O., DANIEL M., KŘIVANEC K. (1977): Findings of soil microscopic fungi in the Himalaya Mountains (Nepal). Česká Mykologie 31(4): 206-213 (published: 1977)
abstract
Within the framework of the 1973 Czechoslovak expedition in the Makalu Mountain region in the Himalayas (East Nepal) a total of 43 soil samples were collected for mycologic examination. Fourteen genera with 37 species of soil fungi were isolated from the samples. Though the spectrum of the isolated microscopic fungi was not wide, it contained 2 genera (Acremonium and Tolypocladium) and 15 species described for the first time amongst the Himalayan mycoflora. Some of the species were collected in the area for the second time. Among the keratinophilic microscopic fungi, Chrysosporium lucknowense was isolated. The investigation and the collection of soil samples took place at the altitudes of 1000–4900 m in the sites where the presence of terrestrial mammals was established, or direct from lair openings. The aim was to detect potentially pathogenic or keratinophilic microscopic fungi. Various species of microscopic fungi were isolated for the first time from the extreme climatic conditions of the high mountains characterized by an increased occurrence of UV radiation, great temperature fluctuations, strong air flow, a decrease in the air pressure and oxygen content and extreme abiotic and biotic factors influencing the specific existence of the organisms.
VESELÝ D. (1977): Das Vorkommen von Pythium oligandrum Drechsler auf der Wurzeloberfläche auflaufender Zuckerrüben in Böhmen und einige Erkenntnisse zur Biologie des Pilzes. Česká Mykologie 31(1): 41-51 (published: 1977)
abstract
During investigations of colonization of healthy emerged sugar beet roots by microorganisms, the fungus Pythium oligandrum Drechsler was found on the root surface of sugar beet seedlings which already had true leaves, in Semčice (Central Bohemia) in 1972. Infection tests proved a low degree of pathogenicity of this fungus towards sugar beet seedlings. In laboratory conditions, Pythium oligandrum showed strong parasitism against Pythium ultimum Trow and Pythium debaryanum (Hesse) De By., which are significant root rot pathogens of sugar beet. In dual cultures with these fungi, P. oligandrum usually dominated the substrate within 24 hours after inoculation and completely destroyed or strongly suppressed the other fungus growing with it on agar medium. Because Pythium oligandrum exhibits only very weak pathogenicity towards sugar beet but is a strong parasite of P. ultimum and P. debaryanum, which it shares the rhizosphere with, its potential use for biological control of sugar beet root rot is discussed, using biopreparations applied to sugar beet seed.
SAMŠIŇÁKOVÁ A. (1975): List of strains of entomogenic fungi. Česká Mykologie 29(4): 211-212 (published: 1975)
abstract
A list of strains of entomogenic fungi is provided, mostly Deuteromycetes, from the mycological collection of the Institute of Entomology, Czechoslovak Academy of Sciences. Most were isolated in Czechoslovakia, some from Cuba and Poland.
ČAPEK A., HANČ O. (1974): Biotransformation of steroids-chemotaxonomic characteric of lower fungi. Česká Mykologie 28(3): 169-172 (published: 1974)
abstract
The mycoflora of southernmost Slovakia, between Nové Zámky, Komárno, and Štúrovo, differs significantly from the rest of the country and resembles Pannonian regions. The author reports 54 herbarium-archived species, including many rare taxa. The area's rich diversity of gilled fungi and Geastrum species is emphasized.
FASSATIOVÁ O., MÁCA B., SVÁTA V., URBAN Z. (1974): Armillaria mellea (Vahl. ex Fr.) Kumm. in coal mines of Kladno (Bohemia). Česká Mykologie 28(1): 35-43 (published: 1974)
abstract
Extensive growths of Armillaria mellea rhizomorphs were observed in timber supports in a coal mine shaft in Kladno, Bohemia. Laboratory and in situ experiments on wood protection were conducted. The fungicides Lastanox Q and Universal proved effective. Imperfect fungi such as Trichoderma viride, Aspergillus niger, and Cladosporium avellaneum also showed inhibitory effects on rhizomorph development.
FASSATIOVÁ O. (1970): Micromycetes unhabiting the mines of Příbram (Czechoslovakia). Česká Mykologie 24(3): 162-165 (published: 20th July, 1970)
abstract
Micromycetes isolated from uranium mines in Příbram include frequent pathogenic species like Aspergillus fumigatus and A. flavus and rare saprophytes such as Scopulariopsis acremonium, Geotrichum microsporum, Humicola brunnea var. africana and Tritirachium sp. nov. Isolations were made from various substrates and miners’ laryngeal swabs.
FASSATIOVÁ O. (1969): Bodenmikromyceten im Gebirge Ždánický les (Steinitzer Wald) und in der Steppe bei Pouzdřany (Pausram). Česká Mykologie 23(4): 243-252 (published: 15th October, 1969)
abstract
A total of 122 species of soil micromycetes were identified in 1963 in Ždánický les and the Pouzdřany steppe (South Moravia), including 25 species of Mucorales and 57 of Moniliales. Species diversity was proportional to soil temperature but not to humidity. Steppe was dominated by Aspergillus and Fusarium; forests by Chrysosporium pannorum.
KOTÝNKOVÁ-SYCHROVÁ E. (1966): The mycoflora of bark-beetle galleries in Czechoslovakia. Česká Mykologie 20(1): 45-53 (published: 1966)
abstract
The author investigated fungi found in galleries of bark beetles in 1961–1962. Several important species were isolated, including Leptographium lundbergii, Ceratocystis minor, C. pilifera, and Graphium pycnocephalum. Most had not been recorded in Czechoslovakia previously.
PŘÍHODA A. (1965): Essai expérimental d’une protection biologique du bois dans les mines. Česká Mykologie 19(3): 175-179 (published: 1965)
abstract
Experimental infections were conducted in the Kladno coal mines using several strains of Trichoderma viride to control wood-decay fungi. Only one strain, isolated from a poplar branch parasitizing Chondroplea populea and Cytospora chrysosperma, was effective against Serpula lacrymans, Heterobasidion annosus, and Fomitopsis pinicola. This early biological wood protection attempt, though discontinued, offers potential for renewed research.
STANĚK M. (1959): The germination of the basidiospores of cultivated mushroom - Agaricus hortensis (Cooke) Pilát. II. The volatile stimulant of germination, produced by mycelium of A. hortensis. Česká Mykologie 13(4): 241-251 (published: 20th October, 1959)
KOCKOVÁ A., KUTKOVÁ M., PETROVÁ M. (1958): Die Arten der Gattung Fusarium, welche die Herzfäulnis der Zuckerrübe im Jahre 1956 in der Slowakei verursachten. Česká Mykologie 12(2): 83-94 (published: 20th April, 1958)
PŘÍHODA A. (1957): Commutationes ligni vi fungorum excitatae earumque classificatio et declaratio. Česká Mykologie 11(3): 183-190 (published: 20th August, 1957)
KOCKOVÁ A., GEBAUEROVÁ A., HRDINOVÁ M. (1956): Tvoření těkavých sloučenin arsenu houbami. Česká Mykologie 10(2): 77-87 (published: 30th June, 1956)
ŠEBEK S. (1954): Poznámky k taxonomii r. Montagnea Fr. Česká Mykologie 8(3): 143-144 (published: 27th August, 1954)
FASSATIOVÁ O. (1954): Houby v chodbách kůrovců. Česká Mykologie 8(3): 138-143 (published: 27th August, 1954)
UHROVÁ-HEJTMÁNKOVÁ N. (1954): O antibiotických účincích metabolických produktů některých našich dřevokazných hub. Česká Mykologie 8(2): 87-92 (published: 28th May, 1954)
PILÁT A. (1952): Rudočechratka tmavá - Rhodopaxillus obscurus Pilát je snad totožná s africkou rudočechratkou černající - Rhodopaxillus nigreescens Maire 1945. Česká Mykologie 6(6-7): 94-97 (published: 15th August, 1952)
FRÁGNER P. (1949): O proměnách plísně Aspergillus chevalieri var. intermedius. Česká Mykologie 3(1-2): 11-13 (published: 15th February, 1949)
CEJP K. (1949): Houbová antibiotika II. Česká Mykologie 3(1-2): 6-11 (published: 15th February, 1949)
CEJP K. (1947): Houbová antibiotika I. Česká Mykologie 1(4): 105-110 (published: 15th December, 1947)
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