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NIGROSPORA":
17 articles found in Index.
JECHOVÁ V. (1963): New species of the genus Nigrospora causing rots of southern fruits - Nigrospora maydis (Garov.) Jechová and N. vietnamensis Jechová. Česká Mykologie 17(1): 12-20 (published: 14th January, 1963)
GRISHKAN I., TEMINA M. (2023): Diversity of microfungal communities inside saxicolous lichens from Nahal Oren, Mount Carmel, Israel. [endolichenic fungi, lichen thallus, melanised fungi, microclimatic contrast, rock surface.] Czech Mycology 75(1): 15-34 (published: 28th March, 2023) Electronic supplement
abstract
In this study, we examined the endolichenic fungal communities of saxicolous lichens covering the rocks in the Nahal Oren valley, northern Israel. A total of 60 fungal species belonging to 35 genera were isolated from six lichen species collected in the summer and winter on the south-facing slope (SFS) and north-facing slope (NFS) of the valley. We verified that rocks serve as a possible source for the formation of endolichenic communities because communities colonising lichen thalli and the rock surface shared 39% of species and clustered together on the SFS. On the NFS, with a comparatively favourable microclimate, lichen thalli abundantly harboured typical soil fungi such as Clonostachys rosea and Fusarium spp. in winter and summer, respectively. At the same time, more severe environmental conditions on the SFS facilitated the prevalence of melanised fungi with thick-walled and multicellular spores irrespective of season. The lowest species richness and isolate densities of endolichenic communities were registered in the thalli of Collema cristatum. This decrease, especially expressed in the summer, was probably associated with the antifungal effect of substances produced by its cyanobiont, as well as with the heavy dehydration of thalli during the dry season.
DEEPTHI A.S., RAY J.G. (2021): Ecological relevance of the endophytic fungal diversity in velamen roots of tropical epiphytic orchids. [epiphytes, Orchidaceae, root-associated endophytes, Trichoderma, Endomelanconiopsis, Lasiodiplodia.] Czech Mycology 73(1): 91-108 (published: 17th May, 2021)
abstract
The velamen roots in epiphytic orchids are rather complex, and their environmental role remains poorly understood. Fungal associates are known in the velamen roots of tropical orchids, but the magnitude of their diversity in particular species remains unknown. The primary aim of this work was to explore endophytic fungal species associated with the velamen roots of three tropical epiphytic orchids. Velamen roots were collected from 30 plants of Rhynchostylis retusa and 25 plants each of Epidendrum radicans and Oncidium sphacelatum. Endophytic fungi were isolated from 2160 segments. Twenty species of velamen-root-associated fungal endophytes were separated with a culture technique. The isolated strains were grouped into morphotypes, subsequently identified morphologically and by means of ITS DNA barcoding. Ascomycota were the dominant group with 18 species. Trichoderma cf. asperellum, Endomelanconiopsis endophytica, Trichoderma cf. atroviride and Lasiodiplodia theobromae were the most frequent taxa isolated. A majority of the identified fungi were common to more than one orchid. Colonisation rate, isolation rate, Shannon-Wiener diversity index, species richness and species evenness of the endophytic fungi from different orchids were studied and were tested for significance with the Kruskal-Wallis H test. The colonisation rate and isolation rate of fungal associates in the velamen roots were found to be distinctly the lowest in Rhynchostylis retusa.
EVALLO E., TAGUIAM J.D., BENGOA J., MAGHIRANG R., BALENDRES M.A. (2021): First report of Colletotrichum fructicola, causing anthracnose of Hylocereus plants, in the Philippines. [Hylocereus undatus, Hylocereus monacanthus, Hylocereus megalanthus, cactus disease, pathogenicity test.] Czech Mycology 73(1): 79-90 (published: 14th April, 2021)
abstract
Cultivation of dragon fruit plants (Hylocereus spp.) in the Philippines has increased over the past years, and its diseases remain one of the major production challenges. In this study, a fungal pathogen, so far unknown in the country, was isolated from H. undatus from anthracnose-like lesion. Isolate MBDF0037C-1 was identified as Colletotrichum fructicola, based on combined morphological and molecular characterisation. This is the first record of C. fructicola causing anthracnose of dragon fruit plants in the Philippines. The isolate caused anthracnose to H. undatus and H. monacanthus, but not to H. megalanthus, in repeated in-vitro and in-vivo pathogenicity tests. Therefore, this study also highlights H. megalanthus as a potential source of resistance to anthracnose in dragon fruit breeding programmes.
VAHEDI-DARMIYAN M.E., JAHANI M., MIRZAEE M.R., ASGARI B. (2017): A noteworthy record of endophytic Quambalaria cyanescens from Punica granatum in Iran. [endophyte, flower, pomegranate, Quambalariaceae.] Czech Mycology 69(2): 113-123 (published: 26th July, 2017)
abstract
During an investigation into endophytic fungi associated with pomegranate in the South Khorasan Province of Iran, 2015–2016, five isolates were recovered with the morphological and molecular characteristics of Quambalaria cyanescens. The present study is the first fully documented report of Q. cyanescens from Iran, providing insight into its geographic distribution and host range. Our study is also the first report of occurrence of Q. cyanescens as an endophyte in a member of the Lythraceae family.
ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt. [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris] Czech Mycology 68(2): 183-214 (published: 20th December, 2016)
abstract
An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.
ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri. [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism] Czech Mycology 67(1): 1-22 (published: 10th April, 2015)
abstract
Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.
ABDULLAH S.K., MONFORT E., ASENSIO L., SALINAS J., LOPEZ LLORCA L.V., JANSSON H.B. (2010): Soil mycobiota of date palm plantations in Elche, SE Spain. [soil saprotrophic fungi, phytopathogenic fungi, Phoenix dactylifera, isolation methods] Czech Mycology 61(2): 149-162 (published: 10th August, 2010)
abstract
The mycobiota of soil from date palm (Phoenix dactylifera) plantations in Elche, SE Spain was examined using 23 soil samples and five isolation methods. One hundred and nineteen species assigned to 67 genera were isolated. The most frequent species were in decreasing order: Aspergillus fumigatus, A. niger, Neosartorya spinosa, Thielaviopsis punctulata, Chaetomium bostrychodes, Gilmaniella macrospora, Aspergillus candidus, Fusarium oxysporum, Rhizopus microsporus, Sordaria fimicola, Aspergillus terreus, Chaetomium murorum, Fusarium solani, Mucor racemosus, Penicillium citrinum and Thielaviopsis paradoxa. The thermotolerant and thermophilic species of Malbranchea cinnamomea, Myriococcum thermophilum, Rhizomucor miehei, Scytalidium thermophilum, Talaromyces emersonii, Thermoascus aurantiacus and Thermomyces lanuginosus were detected in various frequencies of occurrence. Our findings are compared with those from a similar survey of soil from date palm plantations in Iraq. Our study indicates that there is no characteristic mycobiota for soil in date palm plantations except for the more frequent isolation of some species well known as pathogens on date palm.
RAVIKUMAR M., SRIDHAR K.R., SIVAKUMAR T., KARAMCHAND K.S., SIVAKUMAR N., VELLAIYAN R. (2009): Diversity of filamentous fungi on coastal woody debris after tsunami on the southeast coast of India. [tsunami, Bay of Bengal, southeast coast of India, woody litter, fungal diversity] Czech Mycology 61(1): 107-115 (published: 29th September, 2009)
abstract
Five coastal locations on the southeast coast of India severely disturbed after the tsunami on December 26, 2004 were surveyed for the occurrence of filamentous fungi on woody debris by means of short-term (1 month) and long-term (12 months) damp incubation. Short-term incubation revealed 26 mitosporic fungi (8 genera) ranging from 14 to 17 taxa per location with a total frequency of occurrence between 0.4 and 5.6 %. Aspergillus taxa were dominant and six of them were common to all locations.Long-term incubation yielded 35 fungi (25 genera) (22 ascomycetes, 1 basidiomycete and 12 mitosporic fungi). The total fungal taxa per location ranged from 25 to 28 with a total frequency of occurrence of 0.8 to 46.8 %. Corollospora gracilis was the most dominant ascomycete (46.8 %), while Cirrenalia tropicalis and Dictyosporium pelagicum dominated among mitosporic fungi (18 %). The total frequency of occurrence of 11 ascomycetes and four mitosporic fungi was above 10 %. Twelve ascomycetes and two mitosporic fungi were common to all locations. The richness of fungi was higher after long-term than short-term incubation. The occurrence of 61 fungal taxa in this study suggests that the tsunami-dumped woody litter on the southeast coast of India might have at least partially originated from the ocean, thus representing a mosaic of fungi existing in seawater and sediments.
GIRIVASAN K.P., SURYANARAYANAN T.S. (2004): Intact leaves as substrate for fungi: distribution of endophytes and phylloplane fungi in rattan palms. [phylloplane fungi, endophytes, Calamus, India] Czech Mycology 56(1-2): 33-43 (published: 12th August, 2004)
abstract
Leaves of twelve species of Calamus from southern India were screened simultaneously for the presence of phylloplane and endophytic fungi. Sampling of 2400 leaf segments yielded 824 endophyte isolates belonging to 34 species. Thirty species of phylloplane fungi were recorded. Several fungal species were found to be shared as endophytes by different hosts. However, the overlap between endophyte assemblage and phylloplane fungi of each host was low, suggesting that these two distinct groups of fungi occupy different niches, there by avoiding competition.
MARIA G.L., SRIDHAR K.R. (2003): Endophytic fungal assemblage of two halophytes from west coast mangrove habitats, India. [mangroves, halophytes, endophytes, fungi, India] Czech Mycology 55(3-4): 241-251 (published: 22nd December, 2003)
abstract
Twenty-five endophytic fungi comprising three ascomycetes, 20 mitosporic fungi and two sterile fungi were recovered from two halophytes (Acanthus ilicifolius and Acrostichum aureum) of a west coast mangrove habitat in India. Overall colonisation of tissue segments by endophytes ranged between 74.5 % (Acanthus ilicifolius) and 77.5 % (Acrostichum aureum). Analysis using the Jaccard’s similarity coefficient revealed 16-25 % similarity in endophyte assemblage among different tissues, and 24.5 % between the two hosts. Out of four tissues screened, species richness and diversity were high in stems of Acanthus ilicifolius and roots of Acrostichum aureum. The most dominant endophyte was Colletotrichum sp. in prop roots of Acanthus ilicifolius, and Yeast sp. 1 in rhizomes of Acrostichum aureum. Among the dominant endophytes (colonisation frequency >5 %), Acremonium and Yeast sp. 1 were common to both hosts. Acanthus ilicifolius showed dominance of a single species, (Colletotrichum sp.), while in Acrostichum aureum multiple species dominance was seen (Acremonium sp., Pénicillium sp. and Yeast sp. 1). Only one typical marine mitosporic fungus (Cumulospora marina) was recovered from the roots of Acanthus ilicifolius.
Colloquium "Fungi as Model Organisms in Research and Biotechnology - II", Olomouc, Czech Republic, September 5th–6th, 2002. Czech Mycology 55(1-2): 103-149 (published: 23rd July, 2003)
abstract
The colloquium was a continuation of a previous scientific meeting that took place in Olomouc in 1999 (Czech Mycology 52: 139-178, 2000). It was organised by the joint Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology together with the Institute of Biology, Faculty of Medicine of Palacký University, Olomouc. The purpose of the colloquium was to provide a platform for a broad discussion on the use of fungi as model organisms in both basic and applied research. The programme of the colloquium was divided into four parts dealing with the following topics: biochemistry, biotechnology and genetics of fungi; phytopathogenic fungi; fungi pathogenic to humans and animals; and mycology of food and mycotoxins. Each topic was opened with a plenary lecture (30 min.), followed by short communications (10 min.) and accompanied by poster presentations. Besides five plenary lectures, 20 short communications and 24 posters were presented. In total 42 researchers took part in the colloquium and discussed various topics important for the further direction of experimental mycology. Abstracts of the contributions are given here.
GHERBAWY Y.A.M.H., PRILLINGER H. (2000): Root mycoflora of pepper (Capsicum annuum) antagonistic to Verticillium dahliae. [Frequency of root fungi, Verticillium dahliae, Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, Trichoderma harzianum, pepper seed germination] Czech Mycology 52(3): 219-226 (published: 7th December, 2000)
abstract
Thirty-two species belonging to 19 genera of fungi were collected from 30 soil samples from the rhizosphere of pepper plants. The fungal colonies were characterised using classical morphological methods following identification keys. The most frequently isolated fungi were Chaetomiumglobosum, Fusarium oxysporum, Gliocladium roseum, Mucor racemosus, Myrothecium verrucaria, Penicillium aurantiogriseum, P. expansum and Trichoderma harzianum. The crude culture filtrate of Verticillium dahliae at 100% concentration caused sharp decrease in pepper seed germination. Chaetomium globosum, Gliocladium roseum, Myrothecium verrucaria, and Trichoderma harzianum produced a metabolite that retarded radial growth of Verticillium dahliae.
PIECKOVÁ E., JESENSKÁ Z. (1994): The effect of the heat-stable and chloroform-extractable secondary metabolites of filamentous fungi on the respiratory tract cilia movement of one-day-old chickens in vitro. [Fungi, cilia, trachea, chickens, metabolites, bronchitis] Czech Mycology 47(3): 215-221 (published: 17th June, 1994)
abstract
The ciliostatic activity of the heat-stable (100°C/10 min.) and chloroform-extractable metabolites of 63 strains of filamentous fungi - growing on the liquid medium - on tracheal cilia of one-day-old chickens in vitro was evaluated. Twenty two (34.9%) from the investigated strains produced ciliostatic metabolites, 4.7%, 7.9%, 3.1%, resp. 19.0% of the strains stopped the movement of cilia after 24, 48, 72, resp. 144 hours. The results are discussed in connection with chronic bronchitis of people working with moulded materials or living in moulded dwellings.
ŘEPOVÁ A. (1989): Soil micromycetes from Czechoslovakia-a list of isolated species with bibliography. III. Česká Mykologie 44(1): 35-50 (published: 22nd February, 1990)
abstract
A list of micromycetes (saprophytic, keratinophilic, rhizosphere, nematophagous, ovicidal, dermatophytes, and cellular slime moulds) isolated from various Czechoslovak soils is presented. Records about species distribution in Czechoslovakia and bibliography are included for each micromycete species.
SARWAR M. (1974): A new anthracnose disease of Salvia sclarea L. Česká Mykologie 28(3): 156-158 (published: 1974)
abstract
A new anthracnose disease of Salvia sclarea L. was observed in Bangalore, India. Among seven fungi isolated, Colletotrichum dematium was identified as the causal agent. The article details the cultivation of a high-yielding French strain of the plant and describes disease symptoms and environmental conditions under which the disease developed.
FASSATIOVÁ O. (1964): Notes on Humicola Traaen. Česká Mykologie 18(2): 102-108 (published: 16th April, 1964)
abstract
The taxonomic concept of the genus Humicola Traaen is discussed. These soil fungi are highly cellulolytic. Problems related to species differentiation and the genus’s relationship to Monotospora toruloides Corda are mentioned. Traaen's species await thorough revision.
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