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MACROPHOMINA":
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ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt. [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris] Czech Mycology 68(2): 183-214 (published: 20th December, 2016)
abstract
An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.
PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review. [biological control, postharvest diseases, microbial pesticides, rosaceous fruits] Czech Mycology 68(1): 41-66 (published: 1st February, 2016)
abstract
This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.
HASHEM M. (2004): Biological control of two phytopathogenic fungal species isolated from the rhizoplane of soybean (Glycine max). [biological control, soybean, Macrophominaphaseolina, Rhizoctonia solani] Czech Mycology 56(3-4): 223-238 (published: 22nd December, 2004)
abstract
Two hundred isolates representing 31 fungal species (20 genera) were recovered from soybean roots. Samples were collected from 12 localities at 3 different growth stages of the crop. The most dominant species were Aspergillus flavus, Fusarium oxysporum, Fusarium solani (Nectria haematococca), Macrophomina phaseolina and Rhizoctonia solani. Pathogenicity tests have proved the ability of Macrophomina phaseolina and Rhizoctonia solani to infect soybean roots and produce the symptoms of damping-off and root-rot diseases.The efficacy of three antagonists (Trichodermaharzianum, Epicoccum nigrum and Paecilomyceslilacinus) as well as two organic compounds (Strom and F-760) was evaluated as to their control of pathogenic fungi . Biocontrol fungi significantly suppressed Macrophominaphaseolina and Rhizoctonia solani in vitro and in vivo. Epicoccum nigrum and Paecilomyces lilacinus suppressed the growth of the pathogens by producing an inhibition zone while Trichoderma harzianum suppressed them by overgrowing. Strom and F-760 showed lower reduction effect of diseases in comparison with the antagonists.
BABU S., NANDAKUMAR R., SRIRAM S., RAGUCHANDER T., BALASUBRAMANIAN P., SAMIYAPPAN R. (2003): Phytotoxin production and rice sheath blight development by Rhizoctonia solani mutants derived from gamma irradiation. [gamma rays, mutants, phytotoxin, Rhizoctonia solani, rice, sheath blight] Czech Mycology 55(3-4): 261-271 (published: 22nd December, 2003)
abstract
To evaluate the role of the RS-toxin, a phytotoxin produced by the fungus Rhizoctonia solani that causes sheath blight disease of rice, varying doses of gamma irradiation were used to generate mutants of the fungus. All the mutants showed reduced mycelial growth compared to the wild isolate RS7 in liquid culture. The mutants exhibited significant differences in virulence on detached leaf sheath and intact rice plants, the toxin produced and sclerotial formation in culture media and infected leafsheaths. The amount of toxin produced was positively correlated with disease development. SDS-PAGE analysis of mycelial proteins showed that the mutants and wild isolate produced many proteins of different molecular weights at different stages of mycelial growth. In the mutants resulting from higherdoses of gamma rays, the amount of toxin produced and their ability to induce disease symptoms were also greatly reduced.
BANDRE T.R., DAGINAWALA H.F. (1981): Mycoflora associated with various food commodities. Česká Mykologie 35(1): 47-49 (published: 1981)
abstract
Various fungal species were isolated and identified from 11 specimens of food commodities. Since many of the species belong among the potentially toxinogenic ones, the isolates will serve as the source material for the estimation of the real toxinogenity. These estimations are important in the field of hygienic toxicology of foods.
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