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MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt. [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS] Czech Mycology 70(1): 1-32 (published: 26th January, 2018)
abstract
The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).
ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt. [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris] Czech Mycology 68(2): 183-214 (published: 20th December, 2016)
abstract
An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.
SLÁVIKOVÁ E., VADKERTIOVÁ R. (1993): Ecological studies on yeasts in fish-ponds. Česká Mykologie 46(3-4): 272-281 (published: 25th August, 1993)
abstract
Investigations of yeasts isolated from 126 water samples show the occurrence of 16 different species. Two small fish-ponds were sampled twice, in summer and autumn, respectively. The most prevalent species were Aureobasidium pullulans, Sporobolomyces roseus, Hansenula anomala, Hyphopichia burtonii, Leucosporidium scottii, Bullera alba and Candida sp., mainly Candida krusei. The comparison is noted between the yeast population of autumn and summer samples. The total yeast count was 2.5 times higher in autumn than in summer. Isolated yeast strains are characterized by some physiological features.
SLÁVIKOVÁ E., KOVAČOVSKÁ R., KOCKOVÁ-KRATOCHVÍLOVÁ A. (1991): The incidence of yeast organisms in the water of the artificial lake in Jakubov (Slovakia). Česká Mykologie 45(3): 103-111 (published: 31st October, 1991)
abstract
The present paper reports the results of qualitative and quantitative investigation of yeast populations in freshwater of the lake in Jakubov, located in the middle of the Lowland of Záhorie. One hundred and twenty-eight samples offered the possibility to isolate seventy-three yeast strains belonging to twelve genera. Representatives of the genera Candida, Hansenula, Aureobasidium and Rhodotorula occurred most frequently. The occurrence of yeast species at the beginning of the season was twice as heterogeneous as after it. Isolated yeast strains are characterized by some physiological features.
SLÁVIKOVÁ E., KOVAČOVSKÁ R., KOCKOVÁ-KRATOCHVÍLOVÁ A. (1990): On the occurrence of yeasts in fresh-water of the artificial lake in Plavecký Štvrtok. Česká Mykologie 44(3): 152-161 (published: 22nd October, 1990)
abstract
The occurrence of yeasts and yeast-like organisms in the artificial lake at Plavecký Štvrtok (Záhorie Lowland) was studied. Fourteen species were identified from 94 water samples. Most frequent genera included Candida, Hansenula, Aureobasidium, and Rhodotorula. Yeast counts were 3.5 times higher before the tourist season than after. Strains were physiologically characterized.
FRAGNER P. (1985): Keys for identification of yeasts from human material according to new points of view. Česká Mykologie 39(4): 234-242 (published: 8th November, 1985)
abstract
Identification keys were presented for 46 yeasts from human material in this country. Based on the author's own cultures and experience, they conform with the modern monograph by Kreger-van Rij et al. Some new trends and issues in yeast diagnostics are also addressed.
KOCKOVÁ-KRATOCHVÍLOVÁ A., RONALDO CONTRERAS O. (1985): Yeast-like organisms isolated form plant material on Cuba. Česká Mykologie 39(1): 44-50 (published: 1985)
abstract
In 1981, yeast-like organisms were for the first time studied from plant material in Cuba. From 80 samples, 62 isolates were obtained and categorized into six groups: smut-like organisms, apiculate yeasts, Candida and Torulopsis, Rhodotorula, Aureobasidium, and various others. All isolates grew at 42°C and produced a broad spectrum of hydrolytic enzymes.
Varia. Česká Mykologie 38(1): 55-57 (published: 1984)
FRAGNER P. (1981): Further experience with Castellani’s „Water cultures“. Česká Mykologie 35(3): 161-164 (published: 1981)
abstract
Microscopic fungi survive in sterile, distilled water at laboratory temperature for various lengths of time, from less than 11 months (Kloeckera apiculata) to more than 11 years (Sporothrix schenckii). The survival times of some species as well as their spontaneous variability are given. Castellani’s “water cultures” are only an emergency solution for maintaining live collections. Some yeast species which are morphologically or biochemically similar have also similar survival times in water.
FRAGNER P. (1978): Yeasts in human material in our country and their differentiation. Part III. Česká Mykologie 32(3): 144-156 (published: 1978)
abstract
A diagnostic system for the determination of yeasts occurring in human material in our territory is presented. The first part (I) (Ces. Mykol. 32 (1): 32–42, 1978) contained the following paragraphs: Introduction, Material and Methods, Laboratory Studies and Nutrient Media, Survey of the Species, Incidence and Distribution, Key. The second part (II) (Ces. Mykol. 32 (3): 129–143, 1978) contained a detailed characteristics of several species of the genus Candida Berkhout. This part, the third of the series (Part III), contains a characteristics of several species of the genera Cryptococcus Kutzing emend. Vuillemin, Geotrichum Link ex Pers., Kloeckera Janke, Kluyveromyces van der Walt, Rhodotorula Harrison, Saccharomyces (Meyen) Reess and Sporobolomyces Kluyver et van Niel. The descriptions are based on the culture forms occurring in our country. Macroscopic and microscopic appearance, auxanograms, zymograms, growth at 37 °C, data on the pathogenicity for humans and animals as well as these on the origin of the cultures, etc. are given.
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