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GIBBERELLA|zeae":
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Colloquium „Fungi as Model Organisms in Research and Biotechnology - III“ Olomouc, Czech Republic, 2 September 2005. Czech Mycology 57(3-4): 307-323 (published: 10th February, 2006)
abstract
The colloquium was a continuation of the previous scientific meetings that took place in Olomouc in 1999 and 2002 (Czech Mycology 52: 139-178, 2000 and 55: 103-149, 2003). It was organised by the Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology together with the Institute of Biology, Faculty of Medicine of Palacký University, Olomouc. The aim of the colloquium was to provide a platform for a broad discussion on experimental mycology in all branches of basic and applied research. Besides two plenary lectures, 8 short communications and 8 posters were presented. In total 32 researchers from the Czech and Slovak Republics took part in the colloquium and discussed various topics important for the further development of experimental mycology. Abstracts of the contributions are given here.
BABU S., NANDAKUMAR R., SRIRAM S., RAGUCHANDER T., BALASUBRAMANIAN P., SAMIYAPPAN R. (2003): Phytotoxin production and rice sheath blight development by Rhizoctonia solani mutants derived from gamma irradiation. [gamma rays, mutants, phytotoxin, Rhizoctonia solani, rice, sheath blight] Czech Mycology 55(3-4): 261-271 (published: 22nd December, 2003)
abstract
To evaluate the role of the RS-toxin, a phytotoxin produced by the fungus Rhizoctonia solani that causes sheath blight disease of rice, varying doses of gamma irradiation were used to generate mutants of the fungus. All the mutants showed reduced mycelial growth compared to the wild isolate RS7 in liquid culture. The mutants exhibited significant differences in virulence on detached leaf sheath and intact rice plants, the toxin produced and sclerotial formation in culture media and infected leafsheaths. The amount of toxin produced was positively correlated with disease development. SDS-PAGE analysis of mycelial proteins showed that the mutants and wild isolate produced many proteins of different molecular weights at different stages of mycelial growth. In the mutants resulting from higherdoses of gamma rays, the amount of toxin produced and their ability to induce disease symptoms were also greatly reduced.
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