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ŠIMONOVIČOVÁ A. (2001): Supplement to the Checklist of non-vascular and vascular plants of Slovakia. The species of microscopic fungi of the order Eurotiales. [new records of microscopic fungi (Eurotiales), Checklist of non-vascular and vascular plants of Slovakia] Czech Mycology 53(2): 173-188 (published: 20th February, 2002)
abstract
Submited are 50 species of microscopic fungi of the order Eurotiales, which are not listed in the Checklist of non-vascular and vascular plants of Slovakia, part Fungi (Lizoň and Bacigálová 1998). The first group is presented by those microscopic fungi which were isolated only once so far. So we consider them to be scarce or rare. From among 30 species of microscopic fungi more than a halfbelongs to the genus Penicillium (16 species ) or genus Aspergillus (6 species ). The genera Eupenicillium, Eurotium and Paecilomyces have two new species, Emericella and Merimbla only one species. The second group is presented by more frequently isolated species of microscopic fungi. From among 20 species the genus Penicillium dominates with 8 species, followed by the genus Aspergillus with 4 species. Other genera(Byssochlamys, Dichotomomyces, Eupenicillium, Eurotium, Fennellia, Paecilomyces and Talarornyces) are presented with one or two species. From the total number of 50 species of microscopic fungi the prevailing part was isolated from different soils (73.3-75.0 %), from different food stuffs (3.3-30.0 %) and from other sources (23.3-45.0 %), including drinking water, dwellings and different materials in depositories and archives.
OTČENÁŠEK M., DVOŘÁK J. (1985): Fungi infecting man. Taxonomy of agents of human mycoses in an alphabetical survey. Česká Mykologie 39(3): 155-164 (published: 1985)
abstract
An orientational survey of taxonomy of fungi which have been described as agents of human mycoses is given. An alphabetical list of 163 agents contains data on synonyms of the individual species and organs afflicted by them. The literature citation of the earliest paper calling attention to pathogenicity is provided. Current nomenclature of anamorph and teleomorph stages and their mutual relation is discussed.
BHAVANA N.S., PRAKASH H.S., NALINI M.S. (2019): Antioxidative and L-asparaginase potentials of fungal endophytes from Rauvolfia densiflora (Apocynaceae), an ethnomedicinal species of Western Ghats. [India, endophytic fungi, Penicillium chrysogenum, antioxidant, L-asparaginase, phenolic content.] Czech Mycology 71(2): 187-203 (published: 19th December, 2019)
abstract
This study focuses on the isolation and molecular characterisation of fungal endophytes from an ethnomedicinal plant, Rauvolfia densiflora (Apocynaceae), and an evaluation of antioxidative and L-asparaginase potentials. Fungal endophytes were isolated from leaf and stem parts of R. densiflora collected from the site of Talacauvery, Western Ghats, India. The highest colonisation frequency was found in leaves (95.5%), followed by stem parts (63.5%). A total of 19 fungal morphotypes belonging to 11 genera were identified by analysing the ITS sequences of the endophytes. The strains were screened for asparaginase production by qualitative plate assay and quantification by Nesslerisation. Maximum activity was recorded in the Penicillium chrysogenum culture (3.77 IU/ml). Submerged fermentation and ethyl acetate extraction were carried out to obtain secondary metabolites for the evaluation of total phenolic content (TPC) and antioxidative potentials. The TPC of fungal extracts ranged from 12.37 to 89.29 mg GAE/g dry extract and the IC value of scavenging activity from 26.64 to 547.23 μg/ml. The P. chrysogenum strain 50 (MH392736) was found to have the potentially highest total phenolic content and a high antioxidant capacity. This is the first report on the characterisation of fungal endophytes from R. densiflora and their antioxidative and L-asparaginase potentials.
MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt. [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS] Czech Mycology 70(1): 1-32 (published: 26th January, 2018)
abstract
The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).
ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri. [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism] Czech Mycology 67(1): 1-22 (published: 10th April, 2015)
abstract
Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.
TALIGOOLA H.K., ISMAIL M.A., CHEBON S.K. (2011): Mycobiota and aflatoxins associated with imported rice grains stored in Uganda. [rice grain, xerophilic fungi, nephrotoxigenic penicillia, Fusarium, aflatoxins] Czech Mycology 63(1): 93-107 (published: 10th August, 2011)
abstract
Milled rice grains imported into Uganda from Pakistan were investigated for natural contamination by fungi and aflatoxins. The direct plating method using five isolation media was used to enumerate and isolate the fungi during a 270-day storage period. Fungi were isolated and identified to species level and the percentage contamination levels were calculated. A total of 35 species belonging to 16 genera were recorded. The broadest species spectrum were found in the genera Aspergillus, Penicillium, Eurotium and Fusarium, which were represented by 11, 7, 4, and 3 species, respectively. Throughout the storage period, xerophilic fungi including Aspergillus candidus, Eurotium amstelodami and E. chevalieri were predominantly isolated. Species of the genus Penicillium (particularly P. pinophilum) and its teleomorph Talaromyces ranked second in predominance, while Aspergillus flavus, Fusarium spp. and other field fungi occurred only sporadically. Aflatoxins were recorded in rice samples during most storage periods with one sample recording 20–50 ppb. The moisture content increased in rice grains attaining values of over 14 % from the 180th day of storage onwards. A positive correlation was observed between moisture content and incidence of xerophiles, including A. candidus and E. amstelodami.
ABDULLAH S.K., MONFORT E., ASENSIO L., SALINAS J., LOPEZ LLORCA L.V., JANSSON H.B. (2010): Soil mycobiota of date palm plantations in Elche, SE Spain. [soil saprotrophic fungi, phytopathogenic fungi, Phoenix dactylifera, isolation methods] Czech Mycology 61(2): 149-162 (published: 10th August, 2010)
abstract
The mycobiota of soil from date palm (Phoenix dactylifera) plantations in Elche, SE Spain was examined using 23 soil samples and five isolation methods. One hundred and nineteen species assigned to 67 genera were isolated. The most frequent species were in decreasing order: Aspergillus fumigatus, A. niger, Neosartorya spinosa, Thielaviopsis punctulata, Chaetomium bostrychodes, Gilmaniella macrospora, Aspergillus candidus, Fusarium oxysporum, Rhizopus microsporus, Sordaria fimicola, Aspergillus terreus, Chaetomium murorum, Fusarium solani, Mucor racemosus, Penicillium citrinum and Thielaviopsis paradoxa. The thermotolerant and thermophilic species of Malbranchea cinnamomea, Myriococcum thermophilum, Rhizomucor miehei, Scytalidium thermophilum, Talaromyces emersonii, Thermoascus aurantiacus and Thermomyces lanuginosus were detected in various frequencies of occurrence. Our findings are compared with those from a similar survey of soil from date palm plantations in Iraq. Our study indicates that there is no characteristic mycobiota for soil in date palm plantations except for the more frequent isolation of some species well known as pathogens on date palm.
ISMAIL M.A., TALIGOOLA H.K., NAKAMYA R. (2008): Mycobiota associated with baby food products imported into Uganda with special reference to aflatoxigenic aspergilli and aflatoxins. [mycobiota, aflatoxigenic aspergilli, aflatoxins, imported baby foods] Czech Mycology 60(1): 75-89 (published: 4th July, 2008)
abstract
Five baby food products imported into Uganda were collected from different shops and investigated for contamination by fungi and aflatoxins. Forty-two species belonging to 21 genera in addition to some unidentified fungi were recovered on dichloran rose bengal chloramphenicol agar. Cornflakes followed by Cerelac were the most heavily contaminated products while Heinz mixed cereal was the least. Cladosporium sphaerospermum, Fusarium tricinctum and Penicillium oxalicum were the most predominant fungi. On the other hand, using Aspergillus flavus/parasiticus agar, 34 % of the samples were positive for aflatoxigenic aspergilli. Aflatoxigenic aspergilli constituted 78.8 % of all aspergilli and 4.9 % of all contaminating fungi. Samples from all products were contaminated with aflatoxigenic aspergilli, but samples of Cerelac were the most, while those of Porridge oats were the least. Aflatoxin analysis of food samples revealed that 9 out of 13 analysed samples were contaminated with aflatoxins in the range of 1–10 ppb (6 samples) and 11–20 ppb (3 samples). Only samples of Cerelac were aflatoxin–free, although aflatoxigenic aspergilli were detected in some samples. It was noted that samples of Cerelac had the least moisture content compared to the other products. Contaminated foods constitute a health hazard to human consumption. These foods, especially those for babies, must therefore be examined at regular intervals in order to assess their hygienic quality.
LYSKOVÁ P. (2007): Saprotrophic microscopic fungi and dermatophytes accompanying infections of the skin and nails of patients in the Moravian-Silesian Region (Czech Republic). [saprotrophic microscopic fungi, dermatophytes, superficial mycose, Scopulariopsis brevicaulis] Czech Mycology 59(1): 125-137 (published: 28th June, 2007)
abstract
Over a 19-month period, the spectrum of saprotrophic microscopic fungi isolated from 245 patients in the Moravian-Silesian Region (Czech Republic) was analysed. Saprotrophic microscopic fungi were isolated from nails (90 %) and skin (10 %). None was isolated from hair. The material was the most frequently positive for the presence of Scopulariopsis brevicaulis (32.6 %) followed by Cladosporium sphaerospermum (5.3 %), Aspergillus versicolor (4.0 %), Geomyces pannorum (4.0 %) and others.Dermatophytes and saprotrophic microscopic fungi were both studied within one year and represented 1110 isolates. Dermatophytes were isolated in most of the cases and represented 943 isolations (85 %). The saprotrophic microscopic fungus Scopulariopsis brevicaulis is a known causative agent of onychomycosis. In the evaluation including dermatophytes it ended in the 3rd position with 5.2 % of isolations behind Trichophyton rubrum (80 %) and T. mentagrophytes (8 %).
Colloquium „Fungi as Model Organisms in Research and Biotechnology - III“ Olomouc, Czech Republic, 2 September 2005. Czech Mycology 57(3-4): 307-323 (published: 10th February, 2006)
abstract
The colloquium was a continuation of the previous scientific meetings that took place in Olomouc in 1999 and 2002 (Czech Mycology 52: 139-178, 2000 and 55: 103-149, 2003). It was organised by the Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology together with the Institute of Biology, Faculty of Medicine of Palacký University, Olomouc. The aim of the colloquium was to provide a platform for a broad discussion on experimental mycology in all branches of basic and applied research. Besides two plenary lectures, 8 short communications and 8 posters were presented. In total 32 researchers from the Czech and Slovak Republics took part in the colloquium and discussed various topics important for the further development of experimental mycology. Abstracts of the contributions are given here.
NOVÁKOVÁ A., PIŽL V. (2003): Mycoflora in the intestine of Eisenia andrei (Oligochaeta, Lumbricidae) and in vermiculture substrates. [cattle manure, saprotrophic and cellulolytic microfungi, earthworms] Czech Mycology 55(1-2): 83-102 (published: 23rd July, 2003)
abstract
Mycoflora of three commercial vermiculture systems based on cattle manure derived sub strates and Eisenia andrei earthworms was studied using several isolation methods. A total of 172 taxa of saprotrophic micromycetes were isolated (19 taxa of Zygomycetes, 9 taxa of Ascomycetes and 144 taxa of mitosporic fungi). Aspergillus fumigatus was the most frequent microfungal species in the intestine of Eisenia andrei. In vermicuture substrates, Aspergillus flavus and Aspergillus fumigatus were among species isolated very frequently by the soil dilution method, while Rhizopus stolonifer was estimated as frequent species using the soil washing isolation technique.
MOSTAFA M.E., ZOHRI A.A., KOTBY R.S. (2001): Lipid, sterol and ergosterol accumulation in isolates of dematiaceous Hyphomycetes. [Lipid, sterol, ergosterol, dematiaceous hyphomycetes] Czech Mycology 53(2): 149-159 (published: 20th February, 2002)
abstract
Mycelial dry weight, lipid and sterol contents of fungi tested varied with fungalgenus, species and even with isolate of one species. Their dry mass fluctuated between lll.6±l0.7-457.0±41.5 mg/50 ml medium. Lipids, sterols and ergosterol accumulated by the isolates tested ranged from 4.52±0.5-29.04±2.76%, 1.23±0.16-10.63±l.24% and 0.43±0.057-7.13±0.695% of their dry mass, respectively. Cochliobolus spicifer isolate No. 35 was the highest lipid-producer while Ulocladium atrum No. 90 proved to be superior in the production of sterols and ergosterol. TLC technique and chemical analysis of lipid classes produced by U. atrium No. 90 revealed that the lipid fractions are composed of free sterols, free fatty acids, sterol esters, glycolipids, phospholipids and squalene.
GHERBAWY Y.A.M.H., ABDELZAHER H.M.A. (1999): Isolation of fungi from tomato rhizosphere and evaluation of the effect of some fungicides and biological agents on the production of cellulase enzymes by Nectria haematococca and Pythium ultimum var. ultimum. [Biologicalcontrol, root-rot, fungicides Nectria haematococca, Pythium ultimum var. ultimum] Czech Mycology 51(2-3): 157-170 (published: 25th May, 1999)
abstract
Forty-five species and two species varieties belonging to twenty-six genera of fungi were isolated from 30 soil samples from the rhizosphere of tomato plants. The fungi most frequently isolated were Aspergillus flavus, A.fumigatus, A. niger, A. terreus, Gibberella fujikuroi, Nectria haematococca and Rhizopus stolonifer. Ridomil and Vitavax-captan (10, 50 and 100ppm) had no significant effects on the activity of Cl and Cₓ enzymes of Nectria haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but slightly decreased at 100ppm. Vitavax-captan (10, 50 and 100ppm) significantly decreased Cl enzyme activities of N. haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but at 100 ppm it showed a slightly inhibitory effect. Ridomil caused a slight increase in the activity of Cₓ and Cl enzymes by Pythium ultimum var. ultimum at low and moderate doses but the highest dose of Ridomil caused a slight reduction. Vitavax-captan slightly increased the activity of Cₓ and Cl enzymes in P. ultimum var. ultimum. Normal and sterilised filtrates of Myrothecium verrucaria, Penicillium oxalicum and Trichoderma harzianum induced a small decrease in Cl enzyme activity of Nectria haematococca. The sterilised filtrates of the three fungi tested caused greater inhibition compared to the normal filtrate. The production of Cₓ enzyme was slightly increased with normal and sterilised filtrates of Penicillium oxalicum and Trichoderma harzianum, but was significantly increased by both types of filtrates of Myrothecium verrucaria. The two types of filtrate of all fungi tested did not significantly affect the activity of Cl and Cₓ enzymes by lithium ultimum var. ultimum. Production of extracellular protein by Nectria haematococca was not significantly affected by any dose of the tested fungi cides. It was slightly increased by the two types of filtrate of the three tested fungi but significantly increased by the normal filtrate of Myrothecium verrucaria. The normal filtrate of all the fungi tested enhanced extracellular protein production to a greater extent than the sterilised filtrate. Extracellular proteins of Pythium ultimum var. ultimum were slightly increased by all doses of Vitavax-captan and low doses only of Ridomil, also two types of filtrate of all tested fungi caused a slightly increasing effect.
JESENSKÁ Z., PIECKOVÁ E. (1995): Heat-resistant fungi. [Heat resistance, fungi] Czech Mycology 48(1): 73-76 (published: 16th May, 1995)
abstract
The survival of fungi from soil samples has been investigated after temperature of 60, 70, 80 and 90"C in Sabouraud agar. The number of isolated propagules and species had significantly different quantities. The heat-resistant fungi are an economically and scientifically important group of fungi and represent a matter for further investigation.
FRAGNER P. (1991): Identification of Aspergilli isolated from human and animal diseases I. Česká Mykologie 45(3): 113-122 (published: 31st October, 1991)
abstract
A suggestion of simple keys to determination of aspergilli found in human and animal diseases exploits the author’s experience and data from literature. Part I comprises: Introduction, Teleomorphs, Diagnostic possibilities of microscopical findings in clinical specimens, Cultivation, Remark on variability, List of species, Great key, Small key, and Description of species.
ŘEPOVÁ A. (1989): Soil micromycetes from Czechoslovakia - a list of isolated species with bibliography. II. Česká Mykologie 43(4): 235-243 (published: 17th November, 1989)
abstract
A list of micromycetes (saprophytic, keratinophilic, rhizosphere, nematophagous, ovicidal, dermatophytes, and cellular slime moulds) isolated from various Czechoslovak soils is presented. The paper includes distribution data and literature citations for each species. It is the second installment of the series and documents a wide variety of fungi, including Chaetomium species and other frequently encountered genera.
HUBÁLEK Z. (1981): A systematic survey of dimorphic and polymorphic fungi. Česká Mykologie 35(4): 209-226 (published: 1981)
abstract
Various definitions of the term dimorphism in fungi are briefly discussed and a general concept is given with a suggested morphological classification of the dimorphism. Dimorphism (and polymorphism) is delimited as an environmentally controlled reversible phenotypic duality (or plurality in the case of polymorphism) in the morphogenesis of the vegetative fungal cells, which is not restricted to pathogenic species and to the mycelial-yeast transitions only. A systematic list and an alphabetical index of the fungi exhibiting the phenomena of cellular dimorphism or polymorphism are presented.
BANDRE T.R., DAGINAWALA H.F. (1981): Mycoflora associated with various food commodities. Česká Mykologie 35(1): 47-49 (published: 1981)
abstract
Various fungal species were isolated and identified from 11 specimens of food commodities. Since many of the species belong among the potentially toxinogenic ones, the isolates will serve as the source material for the estimation of the real toxinogenity. These estimations are important in the field of hygienic toxicology of foods.
VINDUŠKA L. (1979): Fungi in Heterodera schachtii cysts. Česká Mykologie 33(3): 170-175 (published: 1979)
abstract
In 1976 and 1977 the occurrence of fungi in Heterodera schachtii Schmidt was determined. From 23.6% of cysts individual fungal species were isolated and determined. In 31.8% of cysts fungi did not occur, from 32.2% cysts bacterial colonies grew and in 12.4% of cysts mixtures of fungi and bacteria were found. From the parasitation point of view, the finding of Verticillium falcatum Petch, Cylindrocarpon destructans (Zinssmeister) Schölten, Humicola grisea Traaen, Fusarium oxysporum Schlecht, emend. Snyder et Hansen and Fusarium solani (Mart.) Appel et Wollenw. is important.
CEJP K., MILKO A.A. (1964): Genera of the Eurotiaceae with 32 ascospores-I. Westerdykella. Česká Mykologie 18(2): 82-84 (published: 16th April, 1964)
abstract
A key is given to the known species of the genus Westerdykella Stolk: W. dispersa (Clum) Cejp et Milko comb. nov. (Pycnidiophora dispersa Clum), W. semeonovii Milko, W. multispora (Saito et Minoura) Cejp et Milko comb. nov. (Anixiopsis multispora Saito et Minoura), and W. ornata Stolk, followed by their descriptions and distribution. The genus is characterized by 32 ascospores per ascus and associated imperfect stages like Darluca, parasitic on rusts.
CEJP K. (1963): A peculiar Myxomycete on moudly herbarium material. Česká Mykologie 17(1): 47-48 (published: 14th January, 1963)
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