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20 articles found in Index.
SUKOVÁ M. (2004): Fungi on Juncus trifidus in the Czech Republic. I. [Ascomycetes, anamorphic fungi, Hysteronaevia minutissima, Hysteropezizelladiminuens, Mycosphaerella perexigua var. minima, Naeviella paradoxa, Septoria, taxonomy, ecology] Czech Mycology 56(1-2): 63-84 (published: 12th August, 2004)
abstract
Fungi on Juncus trifidus were collected and studied during they ears 1998-2003, most intensively in 2002. Almost all known localities of this relict plant in the Czech Republic were visited. In this first contribution, 14 species of ascomycetes and anamorphic fungi are mentioned. Populations of Juncus trifidus in the Sudetes and Hercynian mountains are small in comparison with populations in the Alps and Carpathians. However, three species of arcto-alpine fungi (Hysteronaevia minutissima, Hysteropezizella diminuens, Naeviella paradoxa) and Lachnum roseum have been found there as new records for the Czech Republic. The richest localities of fungi on Juncus trifidus are Mt. Sněžka (Krkonoše Mts., Sudetes) and Jezerní stěna rock wall in the cirque of Černé jezero lake (Šumava Mts., Hercynicum).
KOUKOL O., MAGDALINOU E., PÁNKOVÁ H., BOROVIČKA J., MÜNZBERGOVÁ Z. (2022): Do microclimatic conditions in two forest types on serpentine bedrock affect culturable microfungi in pine litter needles? [fungal diversity, ITS rDNA, temperature and humidity, Pinus sylvestris.] Czech Mycology 74(2): 181-194 (published: 24th November, 2022)
abstract
Microfungi colonising coniferous needles in litter were intensively studied in previous decades, but forest stands on serpentine soils have been overlooked. Also, the effects of microclimatic conditions on fungal communities in coniferous litter are unknown. In our study, we aimed to characterise communities of culturable microfungi colonising pine litter needles collected from two types of Scots pine forest growing on serpentine bedrock, i.e. dense forest with relatively stable microclimatic conditions and open-canopy forest on exposed rock with highly variable conditions. The composition of their fungal communities was analysed in respect to microclimatic conditions at the collection sites. Using a combination of phenotypic and molecular data (sequences of ITS rDNA), 35 taxa were distinguished in 1078 fungal colonies recorded, out of which 25 were identified to the species level. Fungal communities were most affected by needle type (litter vs. fermentation layer) followed by maximum temperature during the previous five months. Interestingly, a higher number and abundance of species were recorded at the warmer site, in the open-canopy forest. Dominant fungi recorded in this study (Desmazierella acicola, Phacidium lacerum and Scleroconidioma sphagnicola) were mostly identical to those recorded in previous studies and the occurrence of less abundant taxa previously not recorded in pine litter suggests that the uppermost litter layer represents an important reservoir of fungal diversity.
NOVOTNÝ D. (2022): Contribution to the endophytic mycobiota of aerial parts of oaks. [endophytes, Czech Republic, Quercus robur, Quercus petraea, acorns, seeds, twigs.] Czech Mycology 74(2): 111-121 (published: 1st July, 2022)
abstract
The endophytic mycobiota inhabiting branches of Quercus petraea from two localities in the Křivoklát area, and branches, twigs and acorns of Quercus robur from one locality near the town of Semily were studied (all sites in the Czech Republic). Seventeen fungal taxa were isolated from branches of Q. petraea. Dominant fungi were found to be Colpoma quercinum and Phoma sp. as well as sterile dark mycelium and a black yeast-like fungus close to Sarcinomyces crustaceus. Eighteen fungal taxa were found in samples of aerial parts in Quercus robur. The most frequently isolated taxa were Pezicula cinnamomea, Colpoma quercinum, Alternaria alternata agg. and Acremonium sp.
NOVOTNÝ D., LUKÁŠ J., BROŽOVÁ J., RŮŽIČKOVÁ P. (2019): Comparison of the occurrence of fungi causing postharvest diseases of apples grown in organic and integrated production systems in orchards in the Czech Republic. [Malus domestica, organic production, integrated production, Neofabraea, Penicillium.] Czech Mycology 71(1): 99-121 (published: 28th June, 2019)
abstract
During the years 2013–2015, the occurrence of fungi causing postharvest diseases of apples was evaluated in three apple orchards with integrated and organic production systems in the Czech Republic. The postharvest disease fungi were more frequently recorded in fruits from organically cultivated apple trees (average 29.97%) than those from integrated production (17.67%). This difference is statistically significant. Neofabraea, Penicillium, Alternaria and a sterile grey mycelium were the most frequently recorded taxa in apples grown in organic and integrated production systems. There were also significant differences in the frequency of occurrence of the most frequent fungal taxa between years. In 2013 and 2014, Neofabraea was the most frequently isolated genus in both cultivation systems, whereas in 2015, Penicillium was the most frequently recorded genus also in both cultivation systems, which was likely caused by the low precipitation in the 2015 growing season.
MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): Diversity of yeasts and filamentous fungi in mud from hypersaline and freshwater bodies in Egypt. [fungal diversity, alkalinity, Wadi El-Natrun, lakes, Ibrahimia Canal, River Nile, molecular characterisation, ITS] Czech Mycology 70(1): 1-32 (published: 26th January, 2018)
abstract
The diversity of yeasts and filamentous fungi in muds from hypersaline alkaline lakes of Wadi ElNatrun and fresh water of the Nile River and Ibrahimia Canal was evaluated. The mean pH of saline water mud was 9.21, but fresh water mud registered 8.07. A total of 193 species (two varieties were distinguished in two of them) belonging to 67 genera were recovered from both muds investigated on DRBC (55 genera, 164 species), DG18 (36 genera, 117 species) and MY50G (23 genera, 76 species) media. From these, 17 species assigned to 12 genera were yeasts and 176 species and 2 varieties assigned to 55 genera were filamentous fungi. The highest numbers of fungal propagules were recovered on DRBC from freshwater mud, while the lowest on MY50G from saline water mud. Yeasts constituted a small proportion of all propagules from the two mud types on all three media, whereas filamentous fungi were the major component. However, freshwater mud samples yielded higher numbers of yeast genera and species using all three media. Candida was common in freshwater mud and rare in saline water mud, while Meyerozyma and Rhodotorula were infrequent in both muds. The remaining yeast species were recovered from freshwater mud only. Aspergillus (46 species) was the most common genus of filamentous fungi encountered in all samples, ranging in frequency from 39.82% to 96.62%; A. terreus, A. flavipes and A. niger dominated in both types of mud. Cladosporium (9 species), Fusarium (8 species), Penicillium (18 species) and Scopulariopsis (7 species) were encountered in both types of mud. Notably, 47 filamentous species were isolated only on the media with lower water activity (DG18, MY50G).
PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review. [biological control, postharvest diseases, microbial pesticides, rosaceous fruits] Czech Mycology 68(1): 41-66 (published: 1st February, 2016)
abstract
This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.
ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri. [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism] Czech Mycology 67(1): 1-22 (published: 10th April, 2015)
abstract
Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.
KOUKOL O. (2011): Early spring mycobiota of pine litter needles. [fungal diversity, fungal communities, microfungi, Pinus sylvestris] Czech Mycology 63(2): 153-161 (published: 10th August, 2011)
abstract
Rise in air temperature accompanied by snow-melting in early spring may affect fungi colonising litter needles. This study dealt with the diversity of communities of culturable microfungi colonising Scots pine litter at localities still covered by snow and after snowmelt. Surface-sterilised needles were cultivated on malt agar under two temperature regimes (8/9 and 15/16 °C) and outgrowing fungi were isolated into pure cultures and identified based on morphology and analysis of ITS and partial 28S rDNA regions. Thirty-one fungal species were isolated in total. Dominant colonisers with a colonisation frequency of over 10 % included Verticicladium trifidum, Ceuthospora pinastri, Sympodiella acicola and an unidentified member of Helotiales. Two basidiomycetes, Marasmius androsaceus and Mycena galopus, were also isolated relatively frequently from needles with snow cover, but under different temperature regimes. This preference for different temperatures reflects different niches that these species occupy in coniferous litter. Species richness was significantly higher on needles cultivated at lower temperatures. Widespread use of room temperature for cultivation of samples even from cold seasons may underestimate fungal diversity.
HORTOVÁ B., NOVOTNÝ D. (2011): Endophytic fungi in branches of sour cherry trees: a preliminary study. [Prunus cerasus, Czech Republic, mycobiota, endophytic fungi] Czech Mycology 63(1): 77-82 (published: 10th August, 2011)
abstract
Sour cherry trees (Prunus cerasus L.) harbour various fungal groups, including endophytes. Branches of two different cultivars of sour cherry trees (Újfehértoi Fürtös, Érdi Bötermö) were sampled in May and July 2010 at two study sites in the Czech Republic. Alternaria alternata and coelomycete sp. 1 were the dominant species in branches collected at one site. Aureobasidium pullulans was the most frequent fungal species in branches from the other site. Both species were dominant in May and July.
JANKOWIAK R., ROSSA R., MIŚTA K. (2007): Survey of fungal species vectored by Ips cembrae to European larch trees in Raciborskie forests (Poland). [ophiostomatoid fungi, Ips cembrae, Larix decidua, fungi associated with bark beetles] Czech Mycology 59(2): 227-239 (published: 28th December, 2007)
abstract
The species composition of fungi associated with Ips cembrae was studied in the Raciborskie forests, Poland. The fungi were isolated from overwintered adults, larvae, new adults and from galleries at various stages of development. The results showed that there was a great diversity of fungi associated with this insect. We isolated 2877 cultures, including 61 species. The most important group of fungi were ophiostomatoid species. From these, Ceratocystis laricicola, Ophiostoma brunneo-ciliatum and Graphium laricis were commonly detected, whereas eight other species occurred less frequently or sporadically. Ophiostoma ips is documented here as a new associate of I. cembrae. Ceratocystis laricicola was shown to be the primary invader occurring most frequently at early stages of brood development, particularly in the sapwood. Ophiostoma brunneo-ciliatum and G. laricis were secondary invaders following C. laricicola. In the later stages of brood development other ophiostomatoid fungi appeared.
REMEŠOVÁ J., KOLAŘÍK M., PRÁŠIL K. (2007): Microfungi on the kernels of transgenic and non-transgenic maize damaged by the European corn borer. [Bt-maize, microfungi, plant protection, European corn borer, Zea mays] Czech Mycology 59(2): 205-213 (published: 28th December, 2007)
abstract
From 2002–2004 isolations were carried out to determine the kinds and abundance of microfungi from non-transgenic maize kernels damaged by the European corn borer (ECB) and from transgenic Bt-maize (enriched with delta-endotoxin from the soil bacterium Bacillus thuringiensis). Bt-maize and non-transgenic maize (Zea mays) were grown at Praha-Ruzyně and Ivanovice na Hané, Czech Republic. Thirty-one taxa of filamentous microfungi were isolated, including eight zygomycetes and twenty-three ascomycetes (anamorphic stage). Presence of ECB, corn treatment, year, locality and isolation method significantly accounted for differences in fungus communities. Bt-maize was significantly different from the treatments with non-transgenic hybrids and was often associated with the potentially toxinogenic fungi Alternaria alternata and Epicoccum nigrum. Conversely, Bt-maize had lower incidences of Fusarium spp. and Acremonium strictum.
Colloquium „Fungi as Model Organisms in Research and Biotechnology - III“ Olomouc, Czech Republic, 2 September 2005. Czech Mycology 57(3-4): 307-323 (published: 10th February, 2006)
abstract
The colloquium was a continuation of the previous scientific meetings that took place in Olomouc in 1999 and 2002 (Czech Mycology 52: 139-178, 2000 and 55: 103-149, 2003). It was organised by the Commission for Experimental Mycology of the Czechoslovak Microbiological Society and the Czech Scientific Society for Mycology together with the Institute of Biology, Faculty of Medicine of Palacký University, Olomouc. The aim of the colloquium was to provide a platform for a broad discussion on experimental mycology in all branches of basic and applied research. Besides two plenary lectures, 8 short communications and 8 posters were presented. In total 32 researchers from the Czech and Slovak Republics took part in the colloquium and discussed various topics important for the further development of experimental mycology. Abstracts of the contributions are given here.
HASHEM M. (2004): Biological control of two phytopathogenic fungal species isolated from the rhizoplane of soybean (Glycine max). [biological control, soybean, Macrophominaphaseolina, Rhizoctonia solani] Czech Mycology 56(3-4): 223-238 (published: 22nd December, 2004)
abstract
Two hundred isolates representing 31 fungal species (20 genera) were recovered from soybean roots. Samples were collected from 12 localities at 3 different growth stages of the crop. The most dominant species were Aspergillus flavus, Fusarium oxysporum, Fusarium solani (Nectria haematococca), Macrophomina phaseolina and Rhizoctonia solani. Pathogenicity tests have proved the ability of Macrophomina phaseolina and Rhizoctonia solani to infect soybean roots and produce the symptoms of damping-off and root-rot diseases.The efficacy of three antagonists (Trichodermaharzianum, Epicoccum nigrum and Paecilomyceslilacinus) as well as two organic compounds (Strom and F-760) was evaluated as to their control of pathogenic fungi . Biocontrol fungi significantly suppressed Macrophominaphaseolina and Rhizoctonia solani in vitro and in vivo. Epicoccum nigrum and Paecilomyces lilacinus suppressed the growth of the pathogens by producing an inhibition zone while Trichoderma harzianum suppressed them by overgrowing. Strom and F-760 showed lower reduction effect of diseases in comparison with the antagonists.
SUKOVÁ M., CHLEBICKI A. (2004): Fungi on Juncus trifidus in the Czech Republic (II) with taxonomical notes to some species. [Ascochyta, dark septate endophyte (DSE), Pseudoseptoria, Pycnothyrium, Septoria, Stagonospora, Unguicularia] Czech Mycology 56(3-4): 203-221 (published: 22nd December, 2004)
abstract
In this second contribution, other eight species of ascomycetes and anamorphic fungi on Juncus trifidus collected in the Czech Republic are described (Ascochyta junci, Lachnum diminutum, Phaeosphaeria vagans, Phialocephalasp., Pseudoseptoria sp., Pycnothyrium junci, Stagonosporajunciseda, Unguicularia sp.). Additional localities of Arthrinium cuspidatum and Niptera eriophori described in the first contribution are given. A fungus previously published as Septoria sp. was identified as Septoria chanousiana. Additional material of some fungi (Ascochyta junci, Septoria chanousiana, S. minuta, Unguicularia millepunctata) from other substrata and countries was studied with the aim to compare it with material from Juncus trifidus from the Czech Republic. Numbers of fungi on Juncus trifidus at studied localities are discussed.
PAŽOUTOVÁ S., KOLÍNSKÁ R. (2003): Relationship of Cerebella to Epicoccum and their closest relatives among Dothideales. [Cerebella andropogonis, Epicoccum, phylogeny, rDNA sequence] Czech Mycology 54(3-4): 155-160 (published: 23rd May, 2003)
abstract
The Czech isolate of Cerebella sp. was confirmed as C. and ropogonis, as its RAPD patterns were identical to those of Australian and African isolate of this species. Also, rDNA (ITS1-5.8S-ITS2) sequences of African C. and ropogonis and the Czech isolate (AJ306620 and AJ400905) were identical except for a single transition A-G at position 47 of ITS1. Comparison of these quence with databases yielded 24 closely related sequences with 96.5-98.9 % identity to Cerebella. The highest similarity was found between Cerebella and Epicoccum nigrum/ Phoma epicoccina isolates, two other related groups were : Phoma herbarum, P. medicaginis, Phomopsis sp., and P. glomerata/Ampelomyces sp.
NOVOTNÝ D. (2002): Contribution to the knowledge of the mycoflora in roots of oaks with and without tracheomycotic symptoms. [Quercus robur, Q. petraea, Q. rubra, oak decline, ophiostomatoid fungi, Penicillium, Czech Republic, Moravia] Czech Mycology 53(3): 211-222 (published: 10th January, 2002)
abstract
The mycoflora of roots of three species of oak (Quercus robur, Q. petraea, Q. rubra) from two localities near Moravské Budějovice (southwest Moravia, Czech Republic) with and without tracheomycotic symptoms was studied. Fifty-seven species of fungi were identified from the samples. The most frequently isolated were Fusarium solani, Penicillium gl and icola, P. glabrum, P. simplicissimum and Acremonium curvulum. In the roots of trees with tracheomycotic symptoms two species of ophiostomatoid fungi were recorded. Abiotic condiontions (insufieciency of precipitation and higher average temperature) are considered to be primary reasons of oak decline.
MOSTAFA M.E., ZOHRI A.A., KOTBY R.S. (2001): Lipid, sterol and ergosterol accumulation in isolates of dematiaceous Hyphomycetes. [Lipid, sterol, ergosterol, dematiaceous hyphomycetes] Czech Mycology 53(2): 149-159 (published: 20th February, 2002)
abstract
Mycelial dry weight, lipid and sterol contents of fungi tested varied with fungalgenus, species and even with isolate of one species. Their dry mass fluctuated between lll.6±l0.7-457.0±41.5 mg/50 ml medium. Lipids, sterols and ergosterol accumulated by the isolates tested ranged from 4.52±0.5-29.04±2.76%, 1.23±0.16-10.63±l.24% and 0.43±0.057-7.13±0.695% of their dry mass, respectively. Cochliobolus spicifer isolate No. 35 was the highest lipid-producer while Ulocladium atrum No. 90 proved to be superior in the production of sterols and ergosterol. TLC technique and chemical analysis of lipid classes produced by U. atrium No. 90 revealed that the lipid fractions are composed of free sterols, free fatty acids, sterol esters, glycolipids, phospholipids and squalene.
MARVANOVÁ L., KALOUSKOVÁ V., HANULÁKOVÁ D., SCHÁNĚL L. (1993): Misroscopic fungi in the Zbrašov agaronite caves. Česká Mykologie 46(3-4): 243-250 (published: 25th August, 1993)
abstract
The aragonite coating in some pans of the caves in Zbrašov (northern Moravia, ČSFR) is strewn with fragments of textile fibres, originating probably from the clothes of cave visitors. These filaments as well as the aragonite in their close vicinity are colonised by microscopic fungi. About fifty taxa have been identified. Spores of fungi in the aeroplankton in the caves mostly belong to other species than do occur on the aragonite. The species composition of the fungal community on aragonite is probably influenced by the temperature inside the caves.
ŘEPOVÁ A. (1989): Soil micromycetes from Czechoslovakia - a list of isolated species with bibliography. II. Česká Mykologie 43(4): 235-243 (published: 17th November, 1989)
abstract
A list of micromycetes (saprophytic, keratinophilic, rhizosphere, nematophagous, ovicidal, dermatophytes, and cellular slime moulds) isolated from various Czechoslovak soils is presented. The paper includes distribution data and literature citations for each species. It is the second installment of the series and documents a wide variety of fungi, including Chaetomium species and other frequently encountered genera.
FASSATIOVÁ O., MOŠNOVÁ M. (1979): Epicoccum purpurascens Ehrenb. ex Schlecht. and its sterile mycelium. Česká Mykologie 33(3): 176-180 (published: 1979)
abstract
Epicoccum purpurascens Ehrenb. ex Schlecht. (Dematiaceae) — typical both for its conidia formation in sporodochia and the form of sterile mycelium on agar media — was isolated from various types of forest and steppe soils, withered leaves of common reed (Phragmites communis Trin.), barley grains, from seeds, seedlings and leaves as well as from withered plants of lucerne (Medicago sativa L.), from seeds, seedlings, leaves, roots and dry stems of red clover (Trifolium pratense L.). From seedlings of lucerne and red clover showing symptoms of dwarfing and yellowing, the fungus was isolated as a single microorganism and its potential parasitism should therefore be taken into consideration. Different character of mycelium growth was observed in five artificial media. Sporulation in culture was reached on water and potato-carrot agars after UV irradiation.
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