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EMERICELLA|nidulans var. lata":
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ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri. [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism] Czech Mycology 67(1): 1-22 (published: 10th April, 2015)
abstract
Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.
GHERBAWY Y.A.M.H., ABDELZAHER H.M.A. (1999): Isolation of fungi from tomato rhizosphere and evaluation of the effect of some fungicides and biological agents on the production of cellulase enzymes by Nectria haematococca and Pythium ultimum var. ultimum. [Biologicalcontrol, root-rot, fungicides Nectria haematococca, Pythium ultimum var. ultimum] Czech Mycology 51(2-3): 157-170 (published: 25th May, 1999)
abstract
Forty-five species and two species varieties belonging to twenty-six genera of fungi were isolated from 30 soil samples from the rhizosphere of tomato plants. The fungi most frequently isolated were Aspergillus flavus, A.fumigatus, A. niger, A. terreus, Gibberella fujikuroi, Nectria haematococca and Rhizopus stolonifer. Ridomil and Vitavax-captan (10, 50 and 100ppm) had no significant effects on the activity of Cl and Cₓ enzymes of Nectria haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but slightly decreased at 100ppm. Vitavax-captan (10, 50 and 100ppm) significantly decreased Cl enzyme activities of N. haematococca. Cₓ enzyme activity was slightly increased at 10 and 50ppm, but at 100 ppm it showed a slightly inhibitory effect. Ridomil caused a slight increase in the activity of Cₓ and Cl enzymes by Pythium ultimum var. ultimum at low and moderate doses but the highest dose of Ridomil caused a slight reduction. Vitavax-captan slightly increased the activity of Cₓ and Cl enzymes in P. ultimum var. ultimum. Normal and sterilised filtrates of Myrothecium verrucaria, Penicillium oxalicum and Trichoderma harzianum induced a small decrease in Cl enzyme activity of Nectria haematococca. The sterilised filtrates of the three fungi tested caused greater inhibition compared to the normal filtrate. The production of Cₓ enzyme was slightly increased with normal and sterilised filtrates of Penicillium oxalicum and Trichoderma harzianum, but was significantly increased by both types of filtrates of Myrothecium verrucaria. The two types of filtrate of all fungi tested did not significantly affect the activity of Cl and Cₓ enzymes by lithium ultimum var. ultimum. Production of extracellular protein by Nectria haematococca was not significantly affected by any dose of the tested fungi cides. It was slightly increased by the two types of filtrate of the three tested fungi but significantly increased by the normal filtrate of Myrothecium verrucaria. The normal filtrate of all the fungi tested enhanced extracellular protein production to a greater extent than the sterilised filtrate. Extracellular proteins of Pythium ultimum var. ultimum were slightly increased by all doses of Vitavax-captan and low doses only of Ridomil, also two types of filtrate of all tested fungi caused a slightly increasing effect.
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