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EMERICELLA|heterothallica":
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MOUBASHER A.H., ABDEL-SATER M.A., SOLIMAN Z.S.M. (2018): First records of Aspergillus porphyreostipitatus and Aspergillus carlsbadensis since their original descriptions. [Aspergillus, section Usti, orange plantations, Assiut, Egypt, phenotypic and genotypic characterisation] Czech Mycology 70(1): 67-82 (published: 29th May, 2018)
abstract
During a survey of phyllosphere and non-rhizosphere soil fungi of orange plantations in the Assiut area, Egypt, several isolates of species of Aspergillus belonging to the section Usti were isolated at 25 °C. These were identified using phenotypic and genotypic characters as Aspergillus porphyreostipitatus and Aspergillus carlsbadensis. To the best of our knowledge, these are the first global records since their original descriptions and indicate their probable wide distribution. The strains of both species could grow at 37 °C (a character contrasting to that of the original description of A. carlsbadensis), but both were not able to grow on CYA at 5 °C or 45 °C or to produce acid on creatine. It is interesting to report that both strains produced the urease enzyme (however weakly in A. porphyreostipitatus) and failed to grow on G25N at 25 °C, characters not examined in the original descriptions.
VACCARO G., BENNETT J.W. (1999): Norsolorinic acid mutants and aflatoxin research. [norsolorinic acid, aflatoxins, polyketides, Aspergillusflavus, Aspergillus parasiticus] Czech Mycology 51(2-3): 89-97 (published: 25th May, 1999)
abstract
Norsolorinic acid is a red polyhydroxyanthraquinone. Mutants of Aspergillus parasiticus and Aspergillus flavus that accumulate norsolorinic acid, as well as making low levels of aflatoxin, have been used to study aflatoxin biosynthesis and aflatoxin genetics. In physiological studies the red color of norsolorinic acid serves as a visual screen for the putative presence of aflatoxin. In biosynthetic studies using ¹⁴C-radioisotope labeling, norsolorinic acid is the first stable intermediate detected in the pathway. By complementation of suitable marked strains, and selection for red pigment, the gene for the enzyme associated with norsolorinic acid became the first gene cloned from the aflatoxin pathway. Gene disruption confirmed the role of norsolorinic acid as an aflatoxin precursor. Several laboratories have partially purified an enzyme that catalyzes the transformation of norsolorinic acid to other pigments. It is proposed that double mutants of A. nidulans, lacking both norsolorinic acid production and another secondary metabolite, can be used to screen for regulatory genes of the aflatoxin/sterigmatocystin pathway.
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