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Search for "ASPERGILLUS|carbonarius": 8 articles found in Index.

MARWA ABDEL-KAREEM M., ZOHRI A.A. (2017): Inhibition of three toxigenic fungal strains and their toxins production using selenium nanoparticles.   [Se-NPs, mycotoxigenic moulds, mycotoxins, aflatoxins, ochratoxin A, sterigmatocystin]  Czech Mycology 69(2): 193-204   (published: 24th November, 2017)
  abstract   Spoilage and poisoning of foods by microfungi are a major problem, especially in developing countries. While selenium nanoparticles (Se-NPs) have been used for a wide range of applications including antibacterial, antioxidant and anticancer applications, the effects of Se-NPs on fungal strains remain for the most part unknown to date. Our research is a pioneering attempt to evaluate the antifungal activity and antimycotoxin properties of Se-NPs (32 nm). Se-NPs at different concentrations were evaluated against the growth and mycotoxins production of three toxigenic fungal strains. The growth of Aspergillus parasiticus, A. ochraceus and A. nidulans was completely inhibited using 7000, 9000 and 3000 μg/ml of Se-NPs, respectively, while the complete inhibition in aflatoxins, ochratoxin A and sterigmatocystin production was reported by addition of 2000, 2000, and 800 μg/ml of Se-NPs, respectively. Results of this study show that Se-NPs were effective against the fungal strains tested and their toxin production. These results suggest that Se-NPs could be used as an effective microfungicide in agricultural and food safety applications against toxigenic microfungi.

ABDEL-SATER M.A., MOUBASHER A.H., SOLIMAN Z.S.M. (2016): Diversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in the Assiut region, Egypt.   [Mycobiota, biodiversity, phenotypic and biochemical characterisation, ITS sequence, Coniochaeta canina, Aspergillus stella-maris]  Czech Mycology 68(2): 183-214   (published: 20th December, 2016)
  abstract   An extensive survey of soil mycobiota on citrus and grapevine plantations in Sahel-Saleem City, Assiut Governorate, Egypt was carried out using the dilution-plate method and 2 isolation media at 25 °C. Sixty-four genera and 195 species of filamentous fungi and 10 genera and 13 species of yeasts were recovered. A higher diversity (number of genera and species) and gross total counts were recovered from citrus than from grapevine soil. The peak of filamentous fungi recovered from both soils was found to be in February. Aspergillus (45 species) was the most dominant genus; A. ochraceus predominated in citrus plantations, while A. niger and A. aculeatus in grapevine. The Penicillium count came second after Aspergillus in citrus (23 species) and after Aspergillus and Fusarium in grapevine (11 species).Penicillium citrinum, P. ochrochloron and P. olsonii were more common in citrus plantations, but they were replaced by P. oxalicum in grapevine soil. Fusarium (19 species) was represented in 88.9–100% of both soils on both media; F. solani predominated in both soils, while F. incarnatum came next in citrus, and F. babinda and F. oxysporum in grapevine. Humicola (3 species) with the dominant H. fuscoatra was recorded in 61.1–83.3% of soil of both plantations, while Talaromyces (with T. purpureogenus followed by T. pinophilus being the most common) was recorded in 83.3–100% on DRBC and 38.9–50% on DYM from the soil of plantations of both crops. Volutella (5 species) was common in citrus but missing from grapevine soil. The present study reveals that hyaline fungi predominated over dark-coloured ones. Yeasts comprised only minor proportions in both soils (maximum 0.5%). They showed their peak in the soil of citrus plantations in April and in grapevine in February. All species were recovered in one or two samples only. Diutina catenulata, Debaryomyces hansenii, Galactomyces (3 species), Hanseniaspora occidentalis, Kluyveromyces marxianus, Meyerozyma caribbica, and Schwanniomyces pseudopolymorphus were encountered in citrus only, while Cryptococcus laurentii, Pichia kudriavzevii, Meyerozyma guilliermondii and Rhodotorula sp. in grapevine only. Physiological and growth characteristics were obtained for most of the recovered yeasts.

PARVEEN S., WANI A.H., BHAT M.Y., KOKA J.A. (2016): Biological control of postharvest fungal rots of rosaceous fruits using microbial antagonists and plant extracts - a review.   [biological control, postharvest diseases, microbial pesticides, rosaceous fruits]  Czech Mycology 68(1): 41-66   (published: 1st February, 2016)
  abstract   This article aims to give a comprehensive review on the use of microbial antagonists (fungi and bacteria), botanicals and compost extracts as biocontrol agents against different pathogenic fungi causing postharvest fungal rots in rosaceous fruits which shows that they can play an important role in the biomanagement of fungi causing rot diseases. Plant extracts reported in the literature against pathogenic fungi indicate that they can act as a good biological resource for producing safe biofungicides. However most of the work has been done under experimental conditions rather than field conditions. There is still a need for research to develop suitable formulations of biofungicides from these microbial biocontrol agents and plant extracts. The review reveals that extensive ecological research is also required in order to achieve optimum utilisation of biological resources to manage various postharvest diseases of fruits.

ABDEL-HAFEZ S.I.I., ABO-ELYOUSR K.A.M., ABDEL-RAHIM I.R. (2015): Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri.   [phyllosphere, phylloplane, endophytes, purple blotch disease, antagonism]  Czech Mycology 67(1): 1-22   (published: 10th April, 2015)
  abstract   Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 103 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 103 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.

ABDULLAH S.K., MONFORT E., ASENSIO L., SALINAS J., LOPEZ LLORCA L.V., JANSSON H.B. (2010): Soil mycobiota of date palm plantations in Elche, SE Spain.   [soil saprotrophic fungi, phytopathogenic fungi, Phoenix dactylifera, isolation methods]  Czech Mycology 61(2): 149-162   (published: 10th August, 2010)
  abstract   The mycobiota of soil from date palm (Phoenix dactylifera) plantations in Elche, SE Spain was examined using 23 soil samples and five isolation methods. One hundred and nineteen species assigned to 67 genera were isolated. The most frequent species were in decreasing order: Aspergillus fumigatus, A. niger, Neosartorya spinosa, Thielaviopsis punctulata, Chaetomium bostrychodes, Gilmaniella macrospora, Aspergillus candidus, Fusarium oxysporum, Rhizopus microsporus, Sordaria fimicola, Aspergillus terreus, Chaetomium murorum, Fusarium solani, Mucor racemosus, Penicillium citrinum and Thielaviopsis paradoxa. The thermotolerant and thermophilic species of Malbranchea cinnamomea, Myriococcum thermophilum, Rhizomucor miehei, Scytalidium thermophilum, Talaromyces emersonii, Thermoascus aurantiacus and Thermomyces lanuginosus were detected in various frequencies of occurrence. Our findings are compared with those from a similar survey of soil from date palm plantations in Iraq. Our study indicates that there is no characteristic mycobiota for soil in date palm plantations except for the more frequent isolation of some species well known as pathogens on date palm.

OSTRÝ V., ŠKARKOVÁ J., PROCHÁZKOVÁ I., KUBÁTOVÁ A., MALÍŘ F., RUPRICH J. (2007): Mycobiota of Czech wine grapes and occurrence of ochratoxin A and Alternaria mycotoxins in fresh grape juice, must and wine.   [mycobiota, grapes, grape juice, wine, ochratoxin A, Alternaria mycotoxins, HPTLC]  Czech Mycology 59(2): 241-254   (published: 28th December, 2007)
  abstract   The aim of this study was to monitor the mycobiota of wine grapes, occurrence of ochratoxigenic microfungi in wine grapes and occurrence of ochratoxin A and Alternaria mycotoxins in fresh grape juice, must and wine from domestic crops in the year 2004. Thirteen samples of wine grapes (white /nine samples/ and red /four samples/) were collected during harvesting in the Znojmo wine region, SE Moravia. One sample of a wine grape variety was represented by three subsamples of wine grapes, which were sampled in left, middle and right part of the vineyard. Five wine grape berries per bunch were randomly selected, plated onto Dichloran Rose Bengal Chloramphenicol (DRBC) agar, and incubated for 5–7 days at 25 °C. Alternaria alternata, Cladosporium herbarum, C. cladosporioides, Penicillium expansum, P. aurantiogriseum, P. spinulosum and Rhizopus nigricans were isolated from the samples. Ochratoxigenic microfungi, e. g. Aspergillus carbonarius, and other species of section Nigri, A. ochraceus, Penicillium verrucosum and P. nordicum, were not found in the samples.The HPTLC method for quantification of ochratoxin A (OTA) and Alternaria mycotoxins (alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), and tenuazonic acid (TeA)) in fresh grape juice (13 samples), must (13 samples) and wine (13 samples) was used. The limit of quantification (LoQ) was 8 ng/l for OTA, 1.5 μg/l for AOH, 1.5 μg/l for AME, 1.5 μg/l for ALT and 7.5 μg/l for TeA.Occurrence of OTA and Alternaria mycotoxins in fresh grape juice, must and wine was not proved.

LYSKOVÁ P. (2007): Saprotrophic microscopic fungi and dermatophytes accompanying infections of the skin and nails of patients in the Moravian-Silesian Region (Czech Republic).   [saprotrophic microscopic fungi, dermatophytes, superficial mycose, Scopulariopsis brevicaulis]  Czech Mycology 59(1): 125-137   (published: 28th June, 2007)
  abstract   Over a 19-month period, the spectrum of saprotrophic microscopic fungi isolated from 245 patients in the Moravian-Silesian Region (Czech Republic) was analysed. Saprotrophic microscopic fungi were isolated from nails (90 %) and skin (10 %). None was isolated from hair. The material was the most frequently positive for the presence of Scopulariopsis brevicaulis (32.6 %) followed by Cladosporium sphaerospermum (5.3 %), Aspergillus versicolor (4.0 %), Geomyces pannorum (4.0 %) and others.Dermatophytes and saprotrophic microscopic fungi were both studied within one year and represented 1110 isolates. Dermatophytes were isolated in most of the cases and represented 943 isolations (85 %). The saprotrophic microscopic fungus Scopulariopsis brevicaulis is a known causative agent of onychomycosis. In the evaluation including dermatophytes it ended in the 3rd position with 5.2 % of isolations behind Trichophyton rubrum (80 %) and T. mentagrophytes (8 %).

ŘEPOVÁ A. (1989): Soil micromycetes from Czechoslovakia-a list of isolated species with bibliography.  Česká Mykologie 43(3): 169-175   (published: 21st August, 1989)

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