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Search for "BAUDIŠOVÁ D.": 7 articles found.
KLÁN J., ZIMA T., BAUDIŠOVÁ D. (1994): Potentiated effect of ethanol on Amanita phalloides poisoning. [Amanita phalloides, ethanol, poisoning] Czech Mycology 47(2): 145-150 (published: 15th March, 1994)
abstract
Interaction of the effects of death cap and ethanol in rats was studied. Ethanol was found to have no protective effect during poisoning by Amanita phalloides. In contrast, it burdened hepatocytes with its own detoxification and made the absorption of the fungal toxins easier due to a changed membrane fluidity. Besides, ethanol was responsible for an increased deimage to the cellular membranes by free radicals that originated in its metabolism. The potentiated effects of the two noxae is thus defined. Our results suggest that the intoxication by A. phalloides parallelled by digestion of a small dose of an alcoholic drink will have a more serious course and worse prognosis.
KLÁN J., BAUDIŠOVÁ D. (1994): Enzyme N-acetyl-β-D-glucosaminidase (NAG) as an early marker of intoxications by the Cortinarius species (nephrotoxic syndrom). [Cortinarius species, nephrotoxic syndrom, enzyme N-acetyl-β-glucosaminidase] Czech Mycology 47(1): 65-72 (published: 6th January, 1994)
abstract
The enzyme N-acetyl-β-D-glucosaminidase (EC 3.2.1.30; NAG) was evaluated as a marker of intoxications by the Cortinarius mushroom (nephrotoxic syndrom).Enzyme activity wasmeasured in the urine after i.p. applications of Cortinarius orellanus (Fr.) Fr. and C. rubellus Cooke species, respectively, by fluorimetric and/or colorimetric methods. Considerably higher level of the enzyme (up to 50 times) was observed already on the first day after intoxication, when other s markers of renal damage (e.g. hematuria) were without changes. The high level of enzyme activity was detected up to 4th day after intoxication. The picture of intoxication was completed by determination of the urea level in serum and by histological examinations.
KLÁN J., BAUDIŠOVÁ D., SKÁLA Z. (1992): Enzymy activity of mycelial cultures of saprotrophic macromycetes (Basidiomycotina). III. A taxonomic application. Česká Mykologie 46(1-2): 75-85 (published: 1st April, 1993)
abstract
Mycelial cultures of 92 species belonging to 40 genera of saprotrophic basidiomycetous fungi (orders Agaricales, Aphyllophorales, Gastrosporiales, Lycoperdales and Nidulariales) were tested with respect to the production of extracellular hydrolytic enzymes and oxidoreductases (laccase, peroxidase, tyrosinase, diaminooxidase, proteases, amylases, urease, p-cresol oxidases and hydrolyse of tyrosine) using simple plate and spot tests. The results obtained were evaluated by means of factor analysis methods. Distribution of enzyme activities in individual species was discussed.
KLÁN J., BAUDIŠOVÁ D. (1990): Toxicity of Amanita phalloides (Fr.) Link in a vinegar brine. Česká Mykologie 44(4): 225-227 (published: 26th November, 1990)
abstract
The toxicity of Amanita phalloides preserved in vinegar brine was investigated. Amanitins were detected in the water extract (up to 90% of toxins), but only minor levels were found in the brine, and none in fruit body tissue after removal of the brine. Lignin test and chromatographic methods were used.
KLÁN J., BAUDIŠOVÁ D. (1990): Enzyme activity of mycelial cultures of saprotrophic macromycetes (Basidiomycotina and Ascomycotina). II. Methods of oxidoreductases estimation. Česká Mykologie 44(4): 212-219 (published: 26th November, 1990)
abstract
Spot tests and incubation detection methods were used to study extracellular oxidoreductases (tyrosinase, laccase, catalase, peroxidase, glucose-2-oxidase and diamine oxidase) in mycelial cultures of saprotrophic macromycetes (Basidiomycotina and Ascomycotina). Results from 19 species support their use in chemotaxonomy and for ecological studies.
KLÁN J., BAUDIŠOVÁ D. (1990): Enzyme activity of mycelial cultures of saprotrophic macromycetes (Basidiomycotina). I. Methods of hydrolases estimation. Česká Mykologie 44(4): 203-211 (published: 26th November, 1990)
abstract
A plate diffusion method was used to detect hydrolytic enzymes (lecithinaes, lipases, amylases, proteinases, milk clotting enzymes, urease) in mycelial cultures of saprotrophic macromycetes (Basidiomycotina). The results are demonstrated on 21 selected species. These tests can be used for screening high-yield strains and in chemotaxonomic studies.
KLÁN J., BAUDIŠOVÁ D., BENEŠ K. (1989): Cytochemical demonstration of enzymes in hyphae of mycelial cultures of macromycetes (Ascomycotina and Basidiomycotina). I. Esterases and glycosidases. Česká Mykologie 43(1): 30-35 (published: 6th February, 1989)
abstract
Esterases (carboxyl esterase, alkaline and acid phosphatases, aryl sulphatase) and glycosidases (α- and β-glucosidases, α- and β-galactosidases, β-xylosidase and β-glucuronidase) were found in hyphae of 19 mycelial cultures of 16 species of macromycetes by cytochemical methods in situ. The results were evaluated qualitatively at cellular level. α-glucosidase and β-galactosidase can be used for chemotaxonomical purposes.
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