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OSTRÝ V., ŠKARKOVÁ J., PROCHÁZKOVÁ I., KUBÁTOVÁ A., MALÍŘ F., RUPRICH J. (2007): Mycobiota of Czech wine grapes and occurrence of ochratoxin A and Alternaria mycotoxins in fresh grape juice, must and wine. [mycobiota, grapes, grape juice, wine, ochratoxin A, Alternaria mycotoxins, HPTLC] Czech Mycology 59(2): 241-254 (published: 28th December, 2007)
abstract
The aim of this study was to monitor the mycobiota of wine grapes, occurrence of ochratoxigenic microfungi in wine grapes and occurrence of ochratoxin A and Alternaria mycotoxins in fresh grape juice, must and wine from domestic crops in the year 2004. Thirteen samples of wine grapes (white /nine samples/ and red /four samples/) were collected during harvesting in the Znojmo wine region, SE Moravia. One sample of a wine grape variety was represented by three subsamples of wine grapes, which were sampled in left, middle and right part of the vineyard. Five wine grape berries per bunch were randomly selected, plated onto Dichloran Rose Bengal Chloramphenicol (DRBC) agar, and incubated for 5–7 days at 25 °C. Alternaria alternata, Cladosporium herbarum, C. cladosporioides, Penicillium expansum, P. aurantiogriseum, P. spinulosum and Rhizopus nigricans were isolated from the samples. Ochratoxigenic microfungi, e. g. Aspergillus carbonarius, and other species of section Nigri, A. ochraceus, Penicillium verrucosum and P. nordicum, were not found in the samples.The HPTLC method for quantification of ochratoxin A (OTA) and Alternaria mycotoxins (alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), and tenuazonic acid (TeA)) in fresh grape juice (13 samples), must (13 samples) and wine (13 samples) was used. The limit of quantification (LoQ) was 8 ng/l for OTA, 1.5 μg/l for AOH, 1.5 μg/l for AME, 1.5 μg/l for ALT and 7.5 μg/l for TeA.Occurrence of OTA and Alternaria mycotoxins in fresh grape juice, must and wine was not proved.
OSTRÝ V., RUPRICH J., KOŽÍŠEK J. (1998): Determination of toxigenic Fusarium spp. in the domestic wheat - using the ICFM methodological recommendation. [Fusarium spp., wheat, isolation, identification, mycotoxins, food mycology] Czech Mycology 50(4): 313-323 (published: 12th July, 1998)
abstract
Fifty one food wheat samples from three production regions in the Czech Republic have been mycologically examined in this study. Fusarium species were isolated by them ethod of grain rinse with sterile 0.1 % pepton in water and by the method of direct plating of grains after their surface sterilization. Czapek Dox Iprodione Dichlorane Agar (CZID) was used for cultivation. The methodological procedures used issued from there commendation of the International Commission of Food Mycology (ICFM). The identification of the isolated strains has been done accordingto Nelson et al. (1983) and by comparingwith collection strains of the genus Fusarium (Czech Collection of Microorganisms in Brno - CCM). Standardization of the above-mentioned mycological methods in food mycology is necessary for making collaborative studies and also for possibilities of comparison of results obtained in different time. Fusarium spp. isolated from food wheat samples of showed the greatest frequency in the following order: Fusarium graminearum, F. avenaceum, F. sporotrichioides, F. reticulatum and F. solani. The methodological procedure is recommended for determination of toxigenic Fusariumspp. in wheat and other cereals after a harvest and for comparison of results obtained both between individual production regions and in individual years.
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