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MOSTAFA M.E., ZOHRI A.A., KOTBY R.S. (2001): Lipid, sterol and ergosterol accumulation in isolates of dematiaceous Hyphomycetes. [Lipid, sterol, ergosterol, dematiaceous hyphomycetes] Czech Mycology 53(2): 149-159 (published: 20th February, 2002)
abstract
Mycelial dry weight, lipid and sterol contents of fungi tested varied with fungalgenus, species and even with isolate of one species. Their dry mass fluctuated between lll.6±l0.7-457.0±41.5 mg/50 ml medium. Lipids, sterols and ergosterol accumulated by the isolates tested ranged from 4.52±0.5-29.04±2.76%, 1.23±0.16-10.63±l.24% and 0.43±0.057-7.13±0.695% of their dry mass, respectively. Cochliobolus spicifer isolate No. 35 was the highest lipid-producer while Ulocladium atrum No. 90 proved to be superior in the production of sterols and ergosterol. TLC technique and chemical analysis of lipid classes produced by U. atrium No. 90 revealed that the lipid fractions are composed of free sterols, free fatty acids, sterol esters, glycolipids, phospholipids and squalene.
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