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Search for "PIECKOVÁ E.": 7 articles found.
PIECKOVÁ E., JESENSKÁ Z. (2002): Fusarium moniliforme, F. subglutinans and Aspergillus flavus in maize products in Slovakia. [Fusarium moniliforme, F. subglutinans, Aspergillusflavus, mycotoxins, maize] Czech Mycology 53(3): 229-235 (published: 10th January, 2002)
abstract
Ubiquitous microfungi Fusarium moniliforme, F. subglutinans, F. proliferatum represent frequent contaminants of maize products and can produce some mycotoxins: beauvericin, fusaproliferin and, the most important, fumonisins A₁, A₂, B₁-B₄, C₁ etc. Fumonisins are known to cause serious veterinary, and potentially human, mycotoxicosis. The aim of our study was to characterize the incidence of F. moniliforme and F. subglutinans in the presence of Aspergillus flavus in maize products produced in Slovakia during a four-years period. One hundred and forty samples of maize grain, groat, semolina, flour, and 28 samples of maize straw, corn husk, corn silk and soil from the maize fields were mycologically evaluated for the named strains using potato-dextrose agar with 0.02 % chloramphenicol and 0.3 % of 0.2 % Botran and incubation in dark at 25⁰C for 7-10 days. No Fusarium sp. and A. flavus strains were present in 40 % of the maize samples. The highest number of F. monili for me, F. subglutinans and A. flavus isolates were encountered in flour samples in 1996 (4 264 cfu/g on average), in groat in 1998 (17 743.7 cfu/g on average), and in groat in 1996 (353 cfu/g on average). Twenty two per cent A. flavus isolates and 10 F. moniliforme strains were tested for their ability to produce aflatoxins, or fumonisin B₁, in vitro. No aflatoxin-producing A. flavus isolate was found, but all tested F. moniliforme strains produced fumonisin B₁ in amounts detectable by TLC. According to the results presented in this paper it is evident that strains of F. moniliforme, F. subglutinans and A. flavus were not very important contaminants of maize products from crops harvested in 1995-98 in Slovakia.
PIECKOVÁ E., JESENSKÁ Z. (1997): The effect of chloroform extracts of micromycete biomass on the movement of tracheal cilia in one-day old chickens in vitro. [Micromycete, biomass, chloroform extract, tracheal cilia] Czech Mycology 50(1): 57-62 (published: 29th September, 1997)
abstract
The ciliostatic effect of metabolites from mycelia and spore biomass of 185 micromycete strains extractable with chlor of orm on tracheal epi the l cilia was investigated in 1-d old chickens in vitro. The strains were isolated from cotton or flax. Extracts of 54 strains (29 %) displayed ciliostatic activity: 16 (9 %), 6 (3 %), and 32 (17 %) strains stopped the movement of cilia after 24, 48, and 72 hours, respectively. There may be relationships between these results and respiratory tract illnesses in people living in mouldy dwellings, working with mouldy materials, or with sick building syndrome.
PIECKOVÁ E., JESENSKÁ Z. (1997): Dichotomomyces cejpii - some characteristics of strains isolated from soil in the Slovak Republic. [Dichotomomyces cejpii, microfungi, hyphal analysis, soil, heat-resistance, mycotoxin] Czech Mycology 49(3-4): 229-237 (published: 23rd May, 1997)
abstract
The growth rate of vegetative hyphae of colonies of 22 Dichotomomyces cejpii (Miľko) Scott strains was determined on Sabouraud agar, on Sabouraud agar with 2 and 4 % NaCl, on Sabouraud agar with pH 4-11 and on a medium with 1 % peptone and 4-30 % saccharose. Dichotomomyces cejpii strains are a new member of heat resistant fungi. In our experiments hyphal growth was limited only on Sabouraud agar with 4% NaCl. Chlor of orm-extractable metabolites which stopped the mobility of tracheal cilia of 1 day old chick in vitro were produced by eighteen (58.5%) out of 31 Dichotomomyces cejpii strains in the mycelium and by 26 (83%) in the medium. One strain (No. 2268) had ciliostatic activity comparable with the activity of important known mycotoxins.
JESENSKÁ Z., PIECKOVÁ E. (1995): Heat-resistant fungi. [Heat resistance, fungi] Czech Mycology 48(1): 73-76 (published: 16th May, 1995)
abstract
The survival of fungi from soil samples has been investigated after temperature of 60, 70, 80 and 90"C in Sabouraud agar. The number of isolated propagules and species had significantly different quantities. The heat-resistant fungi are an economically and scientifically important group of fungi and represent a matter for further investigation.
JESENSKÁ Z., VOLNÁ F., PIECKOVÁ E. (1994): The effect of disinfection substances on the propagules of heat-resistant fungi in vitro. [Heat-resistant fungi, disinfection substances] Czech Mycology 47(4): 303-309 (published: 16th February, 1995)
abstract
Inoculum from the strains of the rmoresistant strains of the fungi Botryotrichum (Bo.) piluliferum, Byssochlamys (B.) fulva, B. nivea, Neosartorya (N.) fischeri, Talaromyces (T.) avellaneus, T. bacillisporus, T. flavus and T. trachyspermus consisting from the mixture of mycelium, spores, asci, ascospores, kleistothecia or aleuriospores was exposed in vitro to the action of 7 various types of disinfection solutions, the exposure time being 15 and 60 minutes. Under the experimental conditions, the most effective solutions proved to be the 0.2% Persteril and 1% Septonex solutions, the least effective was 1% Chloramine B solution. Among the tested strains, strain Bo. piluliferum was the most sensitive; B. nivea, B. fulva and N. fischeri were the most resistant strains.
PIECKOVÁ E., JESENSKÁ Z. (1994): The effect of the heat-stable and chloroform-extractable secondary metabolites of filamentous fungi on the respiratory tract cilia movement of one-day-old chickens in vitro. [Fungi, cilia, trachea, chickens, metabolites, bronchitis] Czech Mycology 47(3): 215-221 (published: 17th June, 1994)
abstract
The ciliostatic activity of the heat-stable (100°C/10 min.) and chloroform-extractable metabolites of 63 strains of filamentous fungi - growing on the liquid medium - on tracheal cilia of one-day-old chickens in vitro was evaluated. Twenty two (34.9%) from the investigated strains produced ciliostatic metabolites, 4.7%, 7.9%, 3.1%, resp. 19.0% of the strains stopped the movement of cilia after 24, 48, 72, resp. 144 hours. The results are discussed in connection with chronic bronchitis of people working with moulded materials or living in moulded dwellings.
JESENSKÁ Z., PIECKOVÁ E., BERNÁT D. (1993): Relationships of heat resistant micromycetes from soil to sucrose, natrium chloride and pH. Česká Mykologie 46(3-4): 263-271 (published: 25th August, 1993)
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