The enzyme N-acetyl-β-D-glucosaminidase (EC 3.2.1.30; NAG) was evaluated as a marker of intoxications by the Cortinarius mushroom (nephrotoxic syndrom).Enzyme activity wasmeasured in the urine after i.p. applications of Cortinarius orellanus (Fr.) Fr. and C. rubellus Cooke species, respectively, by fluorimetric and/or colorimetric methods. Considerably higher level of the enzyme (up to 50 times) was observed already on the first day after intoxication, when other s markers of renal damage (e.g. hematuria) were without changes. The high level of enzyme activity was detected up to 4th day after intoxication. The picture of intoxication was completed by determination of the urea level in serum and by histological examinations.
Klán J., Baudišová D. (1994): Enzyme N-acetyl-β-D-glucosaminidase (NAG) as an early marker of intoxications by the Cortinarius species (nephrotoxic syndrom). – Czech Mycology 47(1): 65–72.
